A5087475016- Epigenetics and DNA Methylation
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Mitochondrial Function and Pathology
- Physiological and biochemical adaptations
- Hippo pathway signaling and YAP/TAZ
- Coenzyme Q10 studies and effects
- Protein Hydrolysis and Bioactive Peptides
- Thyroid Disorders and Treatments
- Cardiac Fibrosis and Remodeling
- Cancer-related gene regulation
- Developmental Biology and Gene Regulation
- Hypothalamic control of reproductive hormones
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Genetic Neurodegenerative Diseases
- Animal Genetics and Reproduction
- Congenital Heart Disease Studies
- Receptor Mechanisms and Signaling
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Birth, Development, and Health
- Neuropeptides and Animal Physiology
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
University of California, San Francisco
2017-2021
Broad Center
2019-2021
Hiroshima University
2011-2014
Graduate School USA
2013
Tissue regenerative potential displays striking divergence across phylogeny and ontogeny, but the underlying mechanisms remain enigmatic. Loss of mammalian cardiac correlates with cardiomyocyte cell-cycle arrest polyploidization as well development postnatal endothermy. We reveal that diploid abundance 41 species conforms to Kleiber's law-the ¾-power law scaling metabolism bodyweight-and inversely standard metabolic rate, body temperature, serum thyroxine level. Inactivation thyroid hormone...
The mechanistic target of rapamycin complex1 (mTORC1) signaling pathway has been implicated in functions multicellular processes, including cell growth and metabolism. Although recent reports showed that many pathways, Activin, Bmp, Fgf, sonic hedgehog, Insulin-like factor (IGF), Notch, retinoic acid, Wnt, are non-mammalian vertebrate regeneration, also known as epimorphic mTORC1 function remains unknown. To investigate the role zebrafish caudal fin, we examined activation using an antibody...
Although dedifferentiation, transformation of differentiated cells into progenitor cells, is a critical step in the regeneration amphibians and fish, molecular mechanisms underlying this process, including epigenetic changes, remain unclear. Dot blot assays immunohistochemical analyses revealed that, during zebrafish fin, levels 5-methylcytosine (5mC) 5-hydroxymethylcytosine (5hmC) are transiently reduced blastema adjacent to amputation plane at 30 h post-amputation (hpa), level 5mC, but not...
The mechanisms responsible for active DNA demethylation remain elusive in Metazoa. A previous study that utilized zebrafish embryos provided a potent mechanism which three proteins, AID, MBD4, and GADD45 are involved. We recently found age-dependent hypomethylation zebrafish, it prompted us to examine if AID MBD4 could be involved the phenomenon. Unexpectedly, however, we most of findings were not reproducible. First, injection methylated fragment into eggs did affect either methylation...
Abstract Postnatal mammalian cardiomyocytes undergo a major transition from hyperplasia (increases in cell numbers) to hypertrophy (expansion size). This process is accompanied by rapid mitochondrial biogenesis and metabolic switches meet the demand of increased cardiac output. Although most components express ubiquitously, recent transcriptomic proteomic analyses have discovered numerous tissue-specific proteins whose physiological functions are largely unknown. Here we report that highly...
ABSTRACT Adult mammalian hearts typically have little capacity to regenerate after injuries such as myocardial infarction. In contrast, neonatal mice during the first week of life possess an incredible ability their hearts, though this is lost shortly birth. The physiological triggers mediating transition remains poorly understood. study, we demonstrate that sympathetic nerve activity promotes cardiomyocyte cell-cycle arrest and binucleation. lacking inputs, observe increased mononucleated...