A5079854160- Agricultural and Food Sciences
- Business and Management Studies
- Seed and Plant Biochemistry
- Science and Education Research
- Enzyme Production and Characterization
- Chemistry Education and Research
- Microbial bioremediation and biosurfactants
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Science and Science Education
- Phytase and its Applications
- Bacterial biofilms and quorum sensing
- Biotechnology and Related Fields
- Education Pedagogy and Practices
- Academic Research in Diverse Fields
- Antimicrobial agents and applications
- Environmental Sustainability and Education
- Health and Lifestyle Studies
- Food Chemistry and Fat Analysis
- Protein Hydrolysis and Bioactive Peptides
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Geography and Environmental Studies
- Food Safety and Hygiene
- Gut microbiota and health
- Botanical Research and Applications
Federal University of São João del-Rei
2015-2024
Instituto Nacional de Metrologia, Qualidade e Tecnologia
2023
Universidade Federal de Minas Gerais
2023
Universidade Federal Fluminense
2023
Nucleus (Norway)
2022
National Council for Scientific and Technological Development
2015
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
2015
Universidade Estadual de Campinas (UNICAMP)
1999-2004
Antimicrobial peptides have been developed based on plant-derived molecular scaffolds for the treatment of infectious diseases. Chenopodin is an abundant seed storage protein in quinoa, Andean plant with high nutritional and therapeutic properties. Here, we used computer- physicochemical-based strategies designed four derived from primary structure Chenopodin. Two reproduce natural fragments 14 amino acids Chenopodin, named Chen1 Chen2, two engineered same length were sequence. The...
Uma das atividades empreendedoras que as universidades vêm realizando é o estímulo à criação de novas empresas, denominadas empresas-filhas universidades. Na Universidade Federal São João del-Rei (UFSJ) observado a escassez informações sobre empreendedorismo e do processo suas empresas-filhas. Assim, obter essas fundamental para aprimoramento ambiente empreendedor formulação estratégias apoio empresas. O objetivo deste estudo foi realizar levantamento perfil da (UFSJ). A metodologia adotada...
A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, crude extract quinoa submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that agglutinates human erythrocytes. Its is inhibited by glucose mannose, remained stable under a wide range pH levels temperatures. found be heterodimeric approximately 60 kDa, consisting subunits 25 kDa 35 kDa. This had its antimicrobial tested against several bacteria strains...
Several sesquiterpene lactone were synthesized and their inhibitive activities on phospholipase A(2) (PLA(2)) from Bothrops jararacussu venom evaluated. Compounds Lac01 Lac02 efficient against PLA(2) edema-inducing, enzymatic myotoxic it reduces around 85% of myotoxicity 70% edema-inducing activity. Lac05-Lac08 presented lower efficiency in inhibiting the biological studied reduce only 15%. The activity was significantly reduced. values inhibition constants (K(I)) for approximately 740 μM,...
An acid phosphatase (EC 3.1.3.2) has been identified and purified from castor bean ( Ricinus communis L., IAC‐80 ) seed through sulphopropyl (SP)‐Sephadex, diethylaminoethyl (DEAE)‐Sephadex, Sephacryl S‐200, Concanavalin A‐Sepharose chromatography. The enzyme was 2 000‐fold to homogeneity, with a final specific activity of 3.8 μkat mg −1 protein. revealed single diffuse band on nondenaturing polyacrylamide gel electrophoresis, at pH 8.3. relative molecular mass, determined by...
to estimate the prevalence of TaqIA, -141C and rs6280 polymorphisms ANKK1, DRD2 DRD3 genes evaluate their association with occurrence metabolic syndrome in patients refractory schizophrenia.cross-sectional study conducted Extended Western Region Minas Gerais, schizophrenic using antipsychotic clozapine. Sociodemographic, clinical, anthropometric, biochemical genetic data were collected. Univariate analysis was performed.seventy-two participated Metabolic Syndrome observed 47.2% them. There...
A novel trypsin inhibitor of protease (CqTI) was purified from Chenopodium quinoa seeds. The optimal extracting solvent 0.1M NaCl pH 6.8 (p < 0.05). extraction time 5h and 90 °C optimum for the recovery C. purification occurred in gel-filtration reverse phase chromatography. CqTI presented active against commercial bovine chymotrypsin had a specific activity 5,033.00 (TIU/mg), which to 333.5-fold. extent determined by SDS-PAGE. an apparent molecular weight approximately 12KDa two bands...
Numerous causes of infection in arthroplasties are related to biofilm formation on implant surfaces. In order circumvent this problem, new alternatives prevent bacterial adhesion biosurfactants-based emerging due low toxicity, biodegradability and antimicrobial activity several biosurfactants. We revised all patents relating biosurfactants applicability orthopedic implants.This work aims evaluate the capability a lipopeptide produced by Bacillus subtilis ATCC 19659 isolates acting as...
A partir da Lei n.º 10.973/2004 veio a criação dos Núcleos de Inovação Tecnológica (NITs), com o propósito equiparar forma tratamento das políticas institucionais inovação, dando aos respectivos Núcleos, maior autonomia atuação. Em se tratando inovação à luz ICTs na mesorregião Campo Vertentes no Estado Minas Gerais confirmou-se que não há mapeamento nesta mesorregião. O objetivo deste estudo foi realizar gerada nas Instituições Ensino Superior públicas e privadas sediadas cidades Gerais. É...
Chenopodium quinoa seeds have high protein content. The nutritional value of is superior compared with traditional cereals. Its essential amino acid composition considered next to the ideal, and its quality matches that milk proteins. In this study, seed storage proteins from were extracted, fractionated, partially purified, characterized. structural characterization was performed by Tricine-SDS-PAGE two-dimensional electrophoresis, it confirmed presence molecular weight 30 7kDa, probably...
Humans need amino acids, especially the essential ones, which are obtained from proteins ingested in diet. With rampant growth of world population, it is necessary to increase protein supplies, those plant origin, now play a major role as food source. Leaves ora-pro-nobis (Pereskia aculeata Miller) high quality source and have been used traditional cuisine Minas Gerais for decades. However, important analyze anti-nutritional and/or toxic factors consider use leaves an alternative nutrients....
Chenopodin is an 11S-type globulin purified from Chenopodium quinoa seeds, which can bind carbohydrates and hemagglutinating human erythrocytes. The present study aimed to evaluate the N-terminal structure of heterodimeric its effects in models inflammation. presented two subunits on has homology with other 92%. decreased paw edema neutrophil recruitment induced by carrageenan mice. Concluding, we demonstrated that exhibits vivo anti-inflammatory activity.
Biosurfactants are natural bioactive compounds produced from fungi, bacteria and plants. These molecules have several properties that enable them to be involved in various industrial applications. The surface-active of biosurfactants allow their use sectors, such as agriculture, health, cosmetics, bioremediation/ petroleum, mining, bioprocess.The objective this study was analyze the patents deposited intellectual property databases for applications areas bioremediation/petroleum,...
The Brazilian Cerrado presents a wide variety of natural products, including the Pequi (Caryocar brasiliense). important factor that determines dietary and nutritional ideality protein is degestitibility. This work aims to evaluate digestibility seeds presence anti-nutritional factors. almonds were extracted, toasted untoasted. Evaluation was structurally characterized by SDS-PAGE 15%, which can be used analyze simulated gastric fluid (SFG) digestion in intestinal (SFI) pepsin...
In this study, the aim was to determine complete sequence of Copaifera langsdorffii trypsin inhibitor (CTI)-1 using 2-dimensional (2D)-PAGE, matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF), and quadrupole time-of-flight (QTOF) spectrometry. Spots A (CTI-1) F (CTI-2) were submitted enzymatic digestions with trypsin, SV8, clostripain. The accurate mass peptide obtained from each digest determined by spectrometry (MS) MALDI-TOF. most abundant peptides purified sequenced...
<p>Um obstáculo para o Brasil ser reconhecido mundialmente por suas pesquisas em biotecnologia está na burocracia de processos a aprovação solicitações, incluindo autorizações comitês ética pesquisa envolvendo seres humanos e animais, acesso ao patrimônio genético nacional, estaduais os pedidos patentes. O objetivo deste trabalho foi apresentar fluxogramas dos trâmites às solicitações nas áreas biológicas afins as documentações exigidas cada caso, modo servir como fonte consulta...