A5086385403- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Diet, Metabolism, and Disease
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Digestive system and related health
- Lipid metabolism and disorders
- Liver physiology and pathology
- Adenosine and Purinergic Signaling
- Cancer Cells and Metastasis
- Child Nutrition and Feeding Issues
- Liver Disease and Transplantation
- Lipid Membrane Structure and Behavior
- Lysosomal Storage Disorders Research
- Protease and Inhibitor Mechanisms
- Alcohol Consumption and Health Effects
- Calcium signaling and nucleotide metabolism
- Peroxisome Proliferator-Activated Receptors
- Glycogen Storage Diseases and Myoclonus
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- ATP Synthase and ATPases Research
Washington University in St. Louis
2018-2024
A nanoreporter noninvasively detects endolysosomal lipids, revealing that short-term changes in diet have enduring effects on hepatic macrophages.
Background and Aims Human transmembrane 6 superfamily 2 ( TM6SF2 ) variant rs58542926 is associated with NAFLD HCC. However, conflicting reports in germline Tm6sf2 knockout mice suggest no change or decreased very low density lipoprotein (VLDL) secretion either unchanged increased hepatic steatosis, fibrosis. We generated liver‐specific Tm6Sf2 (Tm6 LKO) to study VLDL the impact on development progression of NAFLD. Approach Results Two independent lines Tm6 LKO exhibited spontaneous...
The RNA-binding protein Apobec1 complementation factor (A1CF) regulates posttranscriptional ApoB mRNA editing, but the range of RNA targets and long-term effect altered A1CF expression on liver function are unknown. Here we studied hepatocyte-specific A1cf-transgenic (A1cf+/Tg), A1cf+/Tg Apobec1–/–, A1cf–/– mice fed chow or high-fat/high-fructose diets using RNA-Seq, CLIP-Seq, tissue microarrays from human hepatocellular cancer (HCC). exhibited increased hepatic proliferation steatosis, with...
Rationale. RNA binding protein 47 (RBM47) is required for embryonic endoderm development but a role in adult intestine unknown.
Regulating dietary fat absorption may impact progression of nonalcoholic fatty liver disease (NAFLD). Here, we asked if inducible inhibition chylomicron assembly, as observed in intestine-specific microsomal triglyceride (TG) transfer protein knockout mice (Mttp-IKO), could retard NAFLD and/or reverse established fibrosis two models. Mttp-IKO fed a methionine/choline-deficient (MCD) diet exhibited reduced hepatic TGs, inflammation, and fibrosis, associated with oxidative stress downstream...
RNA-binding proteins (RBPs) regulate diverse functions by interacting with target transcripts. Here we present a protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine mRNAs in association ribosomal populations. We describe steps identify specific RBPs RNA targets reflecting variety of developmental, physiological, pathological states. This enables RNP complex isolation from tissue sources (liver small intestine) or populations primary cells (hepatocytes), but not at single-cell...
Cover Figure:Rosenblatt et al investigated the role of racial disparities in access to liver transplantation United States.The above figure depicts listing death ratios (LDR) by state.The higher LDR, more likely patients state are be listed for transplant than die from disease.Black face a disparity with lower LDR.