A5083669402- Plant Molecular Biology Research
- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Pancreatic function and diabetes
- Plant Reproductive Biology
- Freezing and Crystallization Processes
- Microbial Metabolites in Food Biotechnology
- Quantum Dots Synthesis And Properties
- Liver Disease Diagnosis and Treatment
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Seismology and Earthquake Studies
- Sperm and Testicular Function
- Earthquake Detection and Analysis
- Legume Nitrogen Fixing Symbiosis
- Peroxisome Proliferator-Activated Receptors
- Anodic Oxide Films and Nanostructures
- Single-cell and spatial transcriptomics
- Lipid metabolism and disorders
- Heat shock proteins research
- Banana Cultivation and Research
- Lipid metabolism and biosynthesis
- Chalcogenide Semiconductor Thin Films
- Neurological Disease Mechanisms and Treatments
Washington University in St. Louis
2020-2025
University of Oklahoma
1990-2017
Sleep loss is associated with cognitive decline in the aging population and a risk factor for Alzheimer’s disease (AD). Considering crucial role of immunomodulating genes such as that encoding triggering receptor expressed on myeloid cells type 2 (TREM2) removing pathogenic amyloid-β (Aβ) plaques regulating neurodegeneration brain, our aim was to investigate whether how sleep influences microglial function mice. We chronically sleep-deprived wild-type mice 5xFAD mouse model cerebral...
Background and Aims Human transmembrane 6 superfamily 2 ( TM6SF2 ) variant rs58542926 is associated with NAFLD HCC. However, conflicting reports in germline Tm6sf2 knockout mice suggest no change or decreased very low density lipoprotein (VLDL) secretion either unchanged increased hepatic steatosis, fibrosis. We generated liver‐specific Tm6Sf2 (Tm6 LKO) to study VLDL the impact on development progression of NAFLD. Approach Results Two independent lines Tm6 LKO exhibited spontaneous...
Demyelination is a hallmark of multiple sclerosis, leukoencephalopathies, cerebral vasculopathies, and several neurodegenerative diseases. The cuprizone mouse model widely used to simulate demyelination remyelination occurring in these Here, we present high-resolution single-nucleus RNA sequencing (snRNA-seq) analysis gene expression changes across all brain cells this model. We define demyelination-associated oligodendrocytes (DOLs) remyelination-associated MAFBhi microglia, as well...
We investigated the role of Triggering Receptor Expressed on Myeloid cells 2 (TREM2) in myelin regeneration brain. TREM2 is a receptor that activates microglia, which are crucial for clearing debris and promoting remyelination. Previous studies mouse model demyelination induced by copper-chelating agent Cuprizone (CPZ) have shown stimulation with monoclonal antibody reduces demyelination, while deleting Trem2 gene mice impairs Here, we blocked function acutely an during both remyelination...
The CaF2 nano-structures grown by thermal vapor deposition are presented. Significant responsivity improvement (>200%) of mid-infrared PbSe detectors incorporating a 200 nm nano-structured coating was observed. detector provides detectivity 4.2 × 1010 cm · Hz1/2/W at 3.8 μm, which outperforms all the reported un-cooled detectors. Structural investigations show that is constructed tapered-shape nanostructures, creates gradient refractive-index profile. Analogy to moth-eye antireflective...
The soybean gene GmFWL1 (FW2-2-like1) belongs to a plant-specific family that includes the tomato FW2-2 and maize CNR1 genes, two regulators of plant development. In soybean, is specifically expressed in root hair cells response rhizobia nodules. Silencing expression significantly reduced nodule numbers supporting its role during nodulation. While biological has been described, molecular function and, more generally, FW2-2-like proteins unknown. this study, we characterized as membrane...
Bacteria express multiple diverse capsular polysaccharides (CPSs) for protection against environmental and host factors, including the immune system. Using a mouse TCR transgenic CD4+ T cell, BθOM, that is specific B. thetaiotaomicron complete set of single CPS-expressing strains, we ask whether CPSs can modify responses to bacterial Ags. Acapsular thetaiotaomicron, which lacks all CPSs, stimulated BθOM cells more strongly than wild-type Despite similar levels Ag expression, many strains...
The organization of isolated embryo sacs and eggs Plumbago zeylanica was described before after fertilization using microscopic cytochemistry scanning electron microscopy. Major developmental events fertilization, including preferential early embryogenesis, are in sacs. two sperms, one unassociated with vegetative nucleus (S ua ) the other physically associated vn ), fuse nuclei egg central cell, respectively. zygote divides asymmetrically to form a two‐celled embryo, consisting massive...
Insulin secretory granules (SGs) mediate the regulated secretion of insulin, which is essential for glucose homeostasis. The basic machinery responsible this exocytosis consists specific proteins present both at plasma membrane and on insulin SGs. protein composition SGs thus dictates their release properties, yet mechanisms controlling SG formation, determine molecular composition, remain poorly understood. VPS41, a component endolysosomal tethering homotypic fusion vacuole sorting (HOPS)...
The generative cell is initiated as a small, lenticular, unpolarized with wall traceable to two origins: the external segment originates intine, while an inner callose positive forms de novo. As lenticular begins its migration into pollen cytoplasm, becomes polarized both externally and internally, displaying characteristic shape patterns of organelle distribution oriented respect vegetative nucleus independent aperture location. Separation from at end opposite results in elongating...
Some unicellular organisms present challenges to chemical fixations that lead common, yet obvious, artifacts. These can be avoided in entirety by adapting spray-freezing technology ultrarapidly freeze specimens for substitution. To specimens, concentrated suspensions of cells ranging diameter from 0.5–30 μm were sprayed with an airbrush at 140–200 kPa (1.05–1.5 torr; 20.3–29.0 psi) into a nylon mesh transfer basket submerged liquid propane. After freezing, the containing frozen sample was...
Hepatic VLDL assembly is proposed as a two-step process in which small primordial lipoprotein particle formed the smooth ER by cotranslational lipidation of apolipoprotein B (APOB) lipid transfer chaperone microsomal triglyceride protein (MTTP) (1Ginsberg H.N. ApoB SURFs Ride from to Golgi.Cell Metab. 2021; 33: 231-233Abstract Full Text PDF PubMed Scopus (2) Google Scholar). The nascent particles are progressively lipidated (step 2) through fusion with droplets that requires MTTP and other...
The generative cell is initiated as a small, lenticular, unpolarized with wall traceable to two origins: the external segment originates intine, while an inner callose positive forms de novo. As lenticular begins its migration into pollen cytoplasm, becomes polarized both externally and internally, displaying characteristic shape patterns of organelle distribution oriented respect vegetative nucleus independent aperture location. Separation from at end opposite results in elongating...
The organization of isolated embryo sacs and eggs Plumbago zeylanica was described before after fertilization using microscopic cytochemistry scanning electron microscopy. Major developmental events fertilization, including preferential early embryogenesis, are in sacs. two sperms, one unassociated with vegetative nucleus (Sua) the other physically associated (Svn), fuse nuclei egg central cell, respectively. zygote divides asymmetrically to form a two-celled embryo, consisting massive...
Spray freezing unicellular organisms directly from the culture medium is an attractive alternative to other methods of rapid because possibility pre-freezing artifacts minimized. However, utility spray-freezing has been limited due difficulties in obtaining satisfactory large specimens and collecting processing after freezing. Consequently, design many devices somewhat complicated, application often difficult use. A simple, inexpensive apparatus our own was developed with purpose algal...
Plant cell division is a fundamental process of plant development that has been studied by myriad different techniques providing principally two-dimensional information. By using fixatives extract major components the cytoplasmic matrix, essential elements mitotic can be readily visualized in scanning electron microscope, and stereopairs collected to visually reconstruct three-dimensional image. Cells onion ( Allium cepa L.) were selected for this study as being essentially representative...
Some unicellular organisms present challenges to chemical fixations that lead common, yet obvious, artifacts. These can be avoided in entirety by adapting spray-freezing technology ultrarapidly freeze specimens for substitution. To specimens, concentrated suspensions of cells ranging diameter from 0.5–30 μm were sprayed with an airbrush at 140–200 kPa (1.05–1.5 torr; 20.3–29.0 psi) into a nylon mesh transfer basket submerged liquid propane. After freezing, the containing frozen sample was...
Journal Article Using Combined TEM, Raman, XRD, and VNIR techniques to Investigate Secondary Phase Formation Textural Relationships in Brine + Jarosite Experiments Get access K Miller, Miller School of Geology Geophysics, University Oklahoma, 100 E. Boyd St., Rm 710 Norman, OK, USA Search for other works by this author on: Oxford Academic Google Scholar C Phillips-Lander, Phillips-Lander G Strout, Strout SRN Microscopy Laboratory, 770 Van Vleet Oval, J Bishop, Bishop SETI Institute, 189...
Abstract Insulin secretory granules (SGs) mediate the regulated secretion of insulin, which is essential for glucose homeostasis. The basic machinery responsible this exocytosis consists specific proteins present both at plasma membrane and on insulin SGs. protein composition SGs thus dictates their release properties, yet mechanisms controlling SG formation, determines molecular composition, remain poorly understood. VPS41, a component endo-lysosomal tethering HOPS complex, was recently...