A5022670879- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- T-cell and B-cell Immunology
- Liver Disease Diagnosis and Treatment
- CAR-T cell therapy research
- Silicon Carbide Semiconductor Technologies
- Oral microbiology and periodontitis research
- Phagocytosis and Immune Regulation
- Infective Endocarditis Diagnosis and Management
- Hepatitis C virus research
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Streptococcal Infections and Treatments
- Advancements in Semiconductor Devices and Circuit Design
- Adipokines, Inflammation, and Metabolic Diseases
- Oral Health Pathology and Treatment
- Cytomegalovirus and herpesvirus research
- Hepatitis B Virus Studies
- HIV/AIDS oral health manifestations
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Pancreatitis Pathology and Treatment
Washington University in St. Louis
2023-2024
Extensive, large-scale single-cell profiling of healthy human blood at different ages is one the critical pending tasks required to establish a framework for systematic understanding aging. Here, using RNA/T cell receptor (TCR)/BCR-seq with protein feature barcoding, we profiled 317 samples from 166 individuals aged 25-85 years old. From this, generated dataset ∼2 million cells that described 55 subpopulations immune cells. Twelve changed age, including accumulation GZMK
Demyelination is a hallmark of multiple sclerosis, leukoencephalopathies, cerebral vasculopathies, and several neurodegenerative diseases. The cuprizone mouse model widely used to simulate demyelination remyelination occurring in these Here, we present high-resolution single-nucleus RNA sequencing (snRNA-seq) analysis gene expression changes across all brain cells this model. We define demyelination-associated oligodendrocytes (DOLs) remyelination-associated MAFBhi microglia, as well...
Abstract Porphyromonas gingivalis is an oral pathogen that promotes dysbiosis by quenching the bactericidal activity of host immune system while maintaining chronic inflammation, leading to periodontitis. This involves secretion virulence factors such as P. peptidyl arginine deiminase (PPAD), which converts C‐terminal Arg residues bacterial and host‐derived proteins peptides into citrulline. We have previously shown PPAD major fimbriae (containing FimA) are necessary for activate T oll‐ l...
Metabolic dysfunction-associated fatty liver disease (MAFLD) is a common complication of obesity with hallmark feature hepatic steatosis. Recent data from animal models MAFLD have demonstrated substantial changes in macrophage composition the liver. In humans, relationship between heterogeneity and steatosis less clear.
Objective. To analyze the results of antiviral therapy patients with chronic hepatitis D (CHD) in Republic Sakha (Yakutia). Patients and Methods. Interim treatment outcomes over 48 weeks were studied 36 receiving bulevirtide monotherapy (30 patients) combination peginterferon alfa (6 patients); 11 these followed for more than weeks, up to 54–118 (5 6 patients, respectively). Results. After treatment, there was a decrease median HDV RNA level from 7.1 log10 4.6 log10, reduction 2.2 ( p <...
Abstract Neutrophils are highly abundant in the gingival tissues where they play an essential role immune homeostasis by preventing microbial invasion. Here, we show that oral periodontal pathogen Porphyromonas gingivalis utilizes its cysteine proteases (gingipains) to disengage phagosomal antimicrobial capacity. Arginine gingipains a sub-family of trypsin-like produced P. cleave several host proteins at arginine residues. We find RgpB-mediated proteolysis is not limited extracellular or...
Macrophages are innate immune cells present in all tissues, which they participate responses and maintenance of tissue homeostasis. They develop either from embryonic precursors or circulating monocytes, their functions part dictated by origin. We previously observed robust monocyte recruitment contribution to the macrophage pool brown adipose tissue. In particular, monocytes were predicted give rise two phenotypically distinct subsets identifiable CD206 CD226 expression. study, we...
Abstract Chimeric antigen receptor (CAR) engineered T cells often fail to enact effector functions after infusion into patients. Understanding the biological pathways that lead CAR failure is of critical importance in design more effective therapies. We developed and validated an vitro model drives cell dysfunction through chronic activation interrogated how costimulatory domains contribute failure. found dysfunctional CD28-based CARs targeting CD19 bear hallmarks classical exhaustion while...