A5019669640- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inhalation and Respiratory Drug Delivery
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Asthma and respiratory diseases
- HIV-related health complications and treatments
- HIV Research and Treatment
- Mass Spectrometry Techniques and Applications
- Respiratory Support and Mechanisms
- Chronic Disease Management Strategies
- Neuroscience and Neuropharmacology Research
- Analytical Chemistry and Chromatography
- Health Systems, Economic Evaluations, Quality of Life
- Cystic Fibrosis Research Advances
- Advanced Proteomics Techniques and Applications
- Railway Systems and Energy Efficiency
- Emergency and Acute Care Studies
- Amyotrophic Lateral Sclerosis Research
- Receptor Mechanisms and Signaling
- Cardiac Ischemia and Reperfusion
- Parkinson's Disease Mechanisms and Treatments
- Simulation Techniques and Applications
- Glycosylation and Glycoproteins Research
- HIV, Drug Use, Sexual Risk
- Ubiquitin and proteasome pathways
ViiV Healthcare (United Kingdom)
2021-2024
Kliniken Essen-Mitte
2024
ViiV Healthcare (Spain)
2024
United States Department of Health and Human Services
2024
Dalhousie University
2023
University of Phoenix
2022
University of Arizona
2022
GlaxoSmithKline (United Kingdom)
2019-2021
GlaxoSmithKline (Netherlands)
2020
Middlesex University
2018
Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an electron to multiply protonated peptides in radio frequency quadrupole linear ion trap (QLT) induce fragmentation the peptide backbone along pathways that are analogous those observed capture dissociation. Modifications QLT enable this presented, automated acquisition high-quality, single-scan dissociation MS/MS...
Recent advances in phosphopeptide enrichment prior to mass spectrometric analysis show genuine promise for characterization of phosphoproteomes. Tandem spectrometry ions, using collision-activated dissociation (CAD), often produces product ions dominated by the neutral loss phosphoric acid. Here we describe a novel method, termed Pseudo MSn, ion quadrupole trap spectrometers. The method induces collisional activation those resulting from loss(es) acid, following precursor ion. Thus,...
MAPKAP kinase 2 (MK2) is required for tumor necrosis factor synthesis. Tristetraprolin (TTP) binds to the 3'-untranslated region of mRNA and regulates its fate. We identified in vitro vivo phosphorylation sites TTP using nanoflow high pressure liquid chromatography microelectrospray ionization tandem mass spectrometry novel methods direct digestion bound affinity matrices (GSH-beads or anti-Myc linked magnetic beads). MK2Delta3B, activated Escherichia coli by p38alpha, phosphorylates at...
Background— Excessive formation of reactive oxygen species contributes to tissue injury and functional deterioration after myocardial ischemia/reperfusion. Especially, mitochondrial are capable opening the permeability transition pore, a harmful event in cardiac Thioredoxins key players defense against oxidative stress. Mutations thioredoxin reductase (thioredoxin reductase-2, Txnrd2) gene have been recently identified cause dilated cardiomyopathy patients. Here, we investigated whether is...
Methods for the simultaneous identification of interacting proteins and post-translational modifications focal adhesion adapter protein, paxillin, are presented. The strategy includes (1) lower-level, transient transfection FLAG-GFP-Paxillin into HEK293 cells, (2) incubation cells with phosphatase inhibitors prior to lysis, (3) purification paxillin by anti-FLAG immunoprecipitation, (4) analysis peptides generated from on-beads digestion using LTQ−FT or LTQ−ETD mass spectrometry, (5)...
Male germ cell-associated kinase (MAK) and intestinal cell (ICK) are nuclear Cdc2-related kinases with nearly identical N-terminal catalytic domains more divergent C-terminal noncatalytic domains. The domain is also related to mitogen-activated protein (MAPKs) contains a corresponding TDY motif. Nuclear localization of ICK requires subdomain XI interactions the conserved Arg-272, but not activity or, surprisingly, any domain. Further, required for its activation. activated by dual...
Background:We assessed the cost-effectiveness of single-inhaler fluticasone furoate (FF)/ umeclidinium (UMEC)/vilanterol (VI) versus FF/VI or UMEC/VI from a Canadian public healthcare perspective, incorporating data IMPACT trial in chronic obstructive pulmonary disease (COPD) (NCT02164513).Methods: Baseline inputs and treatment effects were populated into validated GALAXY-COPD progression model.Canadian unit costs drug (Canadian dollars [C$], 2017) applied to resource utilization...
Current antiretroviral therapies (ARTs) have improved outcomes for people living with HIV. However, the requirement to adhere lifelong daily oral dosing may be challenging some HIV, leading suboptimal adherence and therefore reduced treatment effectiveness. Treatment long-acting (LA) ART improve health-related quality of life. The objective this study was evaluate cost-effectiveness cabotegravir + rilpivirine (CAB+RPV) LA administered every 2 months (Q2M) compared current as single-tablet...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by progressive loss of motor neurons. However, ALS has been recognized to also involve non-motor systems. Subclinical involvement the autonomic system in described. The recently developed <sup>13</sup>C-octanoic acid breath test allows noninvasive measurement gastric emptying. With this new technique we investigated 18 patients with and 14 healthy volunteers. None had diabetes mellitus or...
Fanconi anemia (FA) is an autosomal recessive disease marked by congenital defects, bone marrow failure, and high incidence of leukemia solid tumors. Eight genes have been cloned, with the accompanying protein products participating in at least two complexes, which appear to be functionally dependent upon one another. Previous studies described chromatin localization FA core complex, except mitosis, associated phosphorylation FANCG (F. Qiao, A. Moss, G. M. Kupfer, J. Biol. Chem....
Purpose: To evaluate the cost-effectiveness of once-daily fluticasone furoate/umeclidinium/vilanterol (FF/UMEC/VI) vs twice-daily budesonide/formoterol (BUD/FOR) in patients with symptomatic chronic obstructive pulmonary disease (COPD) at risk exacerbations, from Spanish National Healthcare System perspective. Patients and Methods: The validated GALAXY-COPD model was used to simulate progression predict healthcare costs, quality-adjusted life years (QALYs), incremental ratios (ICERs) over a...
UK management costs for COPD, estimated at £1.9 billion/year, are rising. In the FULFIL (Lung Function and Quality of Life Assessment in Chronic Obstructive Pulmonary Disease with Closed Triple Therapy) study, single-inhaler triple therapy fluticasone furoate/umeclidinium/vilanterol (100/62.5/25 µg) improved clinical outcomes versus budesonide/formoterol (400/12 patients symptomatic COPD risk exacerbations. We assessed cost-effectiveness treating from a National Health Service perspective. A...
Tristetraprolin (TTP) is the prototype for a family of RNA binding proteins that bind tumor necrosis factor (TNF) messenger AU-rich element (ARE), causing deadenylation TNF poly(A) tail, decay, and silencing protein production. Using mass spectrometry sequencing we identified proteins-1 -4 (PABP1 PABP4) in high abundance good coverage from TTP immunoprecipitates. PABP1 significantly enhanced ARE by EMSA prevented TTP-initiated an vitro macrophage assay stability. Neomycin inhibited...
Injectable antiretroviral treatment (ART) represents a new effective and potentially more convenient alternative to oral ART for people living with HIV (PLWH). This study assessed preferences of PLWH long-acting injectable compared in the Netherlands. A labelled discrete choice experiment presented 12 sets ART. were asked select their preferred ART, described by six attributes: location administration, dosing frequency, risk short-term side effects, drug–drug interaction, forgivability, food...
ABSTRACT Background Despite the increasing availability of oral PrEP in Europe, gaps remain coverage HIV prevention strategies at a population level. Long-acting injectable (LA PrEP) has potential to help provide greater proportions men who have sex with (MSM) and trans people, communities still disproportionately affected by HIV. Methods An online cross-sectional survey was conducted across 20 European countries from October 2023 April 2024. The translated into 22 local migrant languages....
With increasing availability of different treatments for chronic obstructive pulmonary disease (COPD), we sought to understand patient preferences COPD treatment in the UK, USA, and Germany using a discrete choice experiment (DCE).
Selective muscarinic agonists might be useful in the treatment of Alzheimer's disease. Previous studies identified several amidine derivatives as selective and efficacious m1 agonists, using receptor subtypes expressed cell lines. In present studies, functional selectivities, side effect profiles memory-enhancing properties these ligands were examined through a series vitro vivo experiments. CDD-0078, CDD-0097, CDD-0102 behaved partial by stimulating PI turnover rat cortical slices to...
The clinical benefit of once-daily fluticasone furoate/umeclidinium/vilanterol (FF/UMEC/VI) versus twice-daily budesonide/formoterol (BUD/FOR) for patients with symptomatic chronic obstructive pulmonary disease (COPD) was demonstrated in a trial setting (FULFIL [NCT02345161]). lifetime cost-effectiveness analysis FF/UMEC/VI BUD/FOR, based on FULFIL data, is reported here. A previously developed and validated GALAXY-COPD linked-risk equation model used to assess the from UK National Health...
The IMPACT trial demonstrated superior outcomes following 52 weeks of once-daily single-inhaler treatment with fluticasone furoate (FF)/umeclidinium (UMEC)/vilanterol (VI) (100/62.5/25 μg) compared FF/VI (100/25 or UMEC/VI (62.5/25 μg). This study evaluated the cost-effectiveness FF/UMEC/VI for chronic obstructive pulmonary disease (COPD) from a UK National Health Service perspective.Patient characteristics and effects were populated into hybrid decision tree/Markov economic model. Costs...