A5086417397- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Optical Coherence Tomography Applications
- Corneal surgery and disorders
- Photoreceptor and optogenetics research
- Retinal and Macular Surgery
- Ocular Diseases and Behçet’s Syndrome
- Retinopathy of Prematurity Studies
- Ophthalmology and Visual Impairment Studies
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Corneal Surgery and Treatments
- Neuroscience and Neural Engineering
- melanin and skin pigmentation
- Ophthalmology and Eye Disorders
- Intraocular Surgery and Lenses
- Connexins and lens biology
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Ocular Oncology and Treatments
- Ocular and Laser Science Research
- Multiple Sclerosis Research Studies
Hospital Clínico Universitario Lozano Blesa
2016-2025
Universidad de Zaragoza
2015-2025
Instituto de Investigación Sanitaria Aragón
2016-2025
Instituto de Salud Carlos III
2021
University of Wisconsin–Madison
2016
Instituto Aragonés de Ciencias de la Salud
2006-2012
Hospital Universitario Miguel Servet
2002-2011
Ophthalmology Associates (United States)
2009-2010
University of Utah
2004-2009
Abstract Differentiation methods for human induced pluripotent stem cells (hiPSCs) typically yield progeny from multiple tissue lineages, limiting their use drug testing and autologous cell transplantation. In particular, early retina forebrain derivatives often intermingle in cultures, owing to shared ancestry tightly coupled development. Here, we demonstrate that three-dimensional populations of retinal progenitor (RPCs) can be isolated both embryonic hiPSC providing a valuable tool...
Numerous protocols have been described that produce neural retina from human pluripotent stem cells (hPSCs), many of which are based on the culture 3D organoids. While nearly all such methods yield at least partial segments highly mature-appearing retinal structure, variabilities exist within and between organoids can change over a protracted time course differentiation. Adding to this complexity potential differences in composition configuration when viewed across multiple differentiations...
Retinitis pigmentosa includes a group of progressive retinal degenerative diseases that affect the structure and function photoreceptors. Secondarily to loss photoreceptors, there is reduction in vascularization, which seems influence cellular process. Retinal macroglial cells, astrocytes, Müller cells provide support for neurons are fundamental maintaining normal function. The aim this study was investigate evolution changes during degeneration P23H rats.Homozygous line-3 rats aged from P18...
Diabetic retinopathy (DR) significantly impacts vision and quality of life in diabetic patients. This study aimed to evaluate multifocal electroretinogram (mfERG) changes long-term type 1 diabetes mellitus (T1DM) patients without clinical signs DR detect early functional retinal alterations. A prospective observational was conducted involving 46 eyes from 23 T1DM age-matched healthy controls. mfERG assessed using the RETI-port/scan21 following ISCEV protocols. exhibited decreased response...
Abstract The Royal College of Surgeons (RCS) rat has a retinal pigment epithelial cell defect that causes progressive loss photoreceptors. Although it is extensively used in degeneration and repair studies, how photoreceptor affects circuitry not been fully explored. This study examined the changes synaptic connectivity between photoreceptors their target cells using immunocytochemistry correlated these with function electroretinogram (ERG). Immunostaining bassoon synaptophysin (as...
In Brief Purpose: To evaluate the ability of time-domain and Fourier-domain optical coherence tomographies (OCTs) to detect macular retinal nerve fiber layer atrophies in retinitis pigmentosa (RP). test intrasession reproducibility using three OCT instruments (Stratus, Cirrus, Spectralis). Methods: Eighty eyes 80 subjects (40 RP patients 40 healthy subjects) underwent a visual field examination, together with 3 scans optic disk evaluations by same experienced examiner instruments....
To evaluate the preventive effect of tauroursodeoxycholic acid (TUDCA) on photoreceptor degeneration, synaptic connectivity and functional activity retina in transgenic P23H rat, an animal model autosomal dominant retinitis pigmentosa (RP).P23H line-3 rats were injected with TUDCA once a week from postnatal day (P)21 to P120, parallel vehicle-administered controls. At was evaluated by electroretinographic (ERG) recording. The effects number, morphology, integrity, retinal cells characterized...
Saffron, an extract from Crocus sativus, has been largely used in traditional medicine for its antiapoptotic and anticarcinogenic properties. In this work, we investigate the effects of safranal, a component saffron stigmas, attenuating retinal degeneration P23H rat model autosomal dominant retinitis pigmentosa. We demonstrate that administration safranal to homozygous line-3 rats preserves both photoreceptor morphology number. Electroretinographic recordings showed higher a- b-wave...
Vision impairments and blindness caused by retinitis pigmentosa result from severe neurodegeneration that leads to a loss of photoreceptors, the specialized light-sensitive neurons enable vision. Although mammalian nervous system is unable replace lost due degeneration, therapeutic approaches reprogram resident glial cells retinal have been proposed. Here, we demonstrate Müller glia can be reprogrammed in vivo into precursors then differentiate photoreceptors. We transplanted hematopoietic...
To test intrasession, intersession, intervisit and interoperator reproducibility of retinal nerve fibre (RNFL) measurements thickness in healthy subjects using Cirrus Fourier-domain optical coherence tomography (OCT) (Carl Zeiss Meditec, Inc).Seventy-two eyes 72 were included the study. All underwent three 512 × 128 volume cube centred on fovea 360° circular scans optic disc by one observer. This sequence was redone another observer a second visit within 2-week period. Descriptive...
To evaluate the ability of Fourier-domain (FD) optical coherence tomography (OCT) to detect retinal nerve fiber layer (RNFL) atrophy in multiple sclerosis (MS) patients. test intrasession reproducibility RNFL thickness measurements MS and healthy subjects using Cirrus (Carl Zeiss Meditec, Dublin, CA) Spectralis (Heidelberg Engineering, Heidelberg, Germany) OCT.Two hundred twenty-two eyes 111 (50 patients 61 subjects) underwent three 360° circular scans centered on optic disc by same...
Microphthalmia-associated transcription factor (MITF) is a master regulator of pigmented cell survival and differentiation with direct transcriptional links to cycle, apoptosis pigmentation.In mouse, Mitf expressed early uniformly in optic vesicle (OV) cells as they evaginate from the developing neural tube, null mutations result microphthalmia pigmentation defects.However, homozygous MITF have not been identified humans; therefore, little known about its role human retinogenesis.We used...
To investigate the spatiotemporal relationship between rod and cone degeneration in P23H-1 rat.Control Sprague-Dawley (SD) rats of ages ranging from P30 to P365 were used. Retinas processed for whole mounts or cross sections rods cones immunodetected. We used newly developed image analysis techniques quantify total population L/M (the most abundant rat) analyzed rings rod-cone degeneration.In rats, occurs rapidly: first outer segment shortens, at there is extensive loss, by P180 loss almost...
To study the course of photoreceptor cell death and macro microglial reactivity in two rat models retinal degeneration with different etiologies. Retinas from P23H-1 (rhodopsin mutation) Royal College Surgeon (RCS, pigment epithelium malfunction) rats age-matched control animals (Sprague-Dawley Pievald Viro Glaxo, respectively) were cross-sectioned at postnatal ages (from P10 to P60) rhodopsin, L/M- S-opsin, ionized calcium-binding adapter molecule 1 (Iba1), glial fibrillary acid protein...
purpose. Established techniques for culturing primary human retinal pigment epithelial (RPE) cells have facilitated the laboratory investigation of this multipurpose cell layer. However, most culture methods involve use animal serum to establish and maintain RPE monolayers, which can complicate efforts define study factors involved in maturation function these cells. Therefore, was conducted develop a simple, serum-free system propagate sustain vitro. methods. dissected from prenatal donor...
Successful drug therapies for treating ocular diseases require effective concentrations of neuroprotective compounds maintained over time at the site action. The purpose this work was to assess efficacy intravitreal controlled delivery tauroursodeoxycholic acid (TUDCA) encapsulated in poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres treatment retina a rat model retinitis pigmentosa. PLGA (MSs) containing TUDCA were produced by O/W emulsion-solvent evaporation technique. Particle size...