A5101585216- RNA Interference and Gene Delivery
- Corneal Surgery and Treatments
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- Connective Tissue Growth Factor Research
- Protease and Inhibitor Mechanisms
- T-cell and B-cell Immunology
- Advanced Biosensing Techniques and Applications
- Retinoids in leukemia and cellular processes
- Natural product bioactivities and synthesis
- Cerebrovascular and genetic disorders
- Immune Response and Inflammation
- Hippo pathway signaling and YAP/TAZ
- Genomics, phytochemicals, and oxidative stress
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Proteoglycans and glycosaminoglycans research
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Curcumin's Biomedical Applications
- Oral microbiology and periodontitis research
- Photodynamic Therapy Research Studies
- Herpesvirus Infections and Treatments
- Nuclear Receptors and Signaling
Sun Yat-sen University
2000-2022
Sun Yat-sen Memorial Hospital
2001-2022
It has been reported that epithelial-mesenchymal transition (EMT) mediates multiple physiological and pathological processes. However, the occurrence pathogenic role of high glucose-induced EMT in retinal pigment epithelial cells (RPE cells) is unknown. The aim this study was to examine effects glucose on RPE cells. Cultured were exposed 25 mM D-glucose. A vector encoding Snail gene siRNA targeting (Snail siRNA) transfected into induce overexpression or silencing Snail, respectively. AKT...
Müller cells are retinal glial and exhibit a fibroblast-like phenotype ability to migrate in diabetic retinopathy (DR). However, expression of mesenchymal markers, which promote fibrosis various organs, has not been characterized the retina. We examined changes these markers exposed high glucose animal models retinopathy. High conditions increased maker migration cells. Snail, N-cadherin, Vimentin, β-catenin, α-smooth muscle actin (α-SMA) levels were all dramatically retinas from humans with...
Abstract: A hyperbranched cationic polysaccharide derivative-mediated small interfering (si)RNA interference strategy was proposed to inhibit nuclear transcription factor-kappa B (NF-κB) activation in human retinal pigment epithelial (hRPE) cells for the gene therapy of diabetic retinopathy. Two derivatives containing same amount residues, but with different branching structures and molecular weights, including 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) amylopectin...
AIM: To investigate the effects of curcumin (Cur) nanoparticles loaded with chitosan derivatives grafted by deoxycholic acid (Chit-DC) on human retinal pigment epithelial (hRPE) cell proliferation and vascular endothelial growth factor (VEGF) mRNA expression. METHODS: Cur were synthesized Chit-DC as carrier supported drug. Cell counting kit-8 (CCK-8) method was used to detect different concentrations Cur/Chit-DC, Chit-DC, hRPE cells for times. The changes Cur/Chit-DC cycle determined flow...
AIM: Circular RNAs (circRNAs) function as miRNA sponges by adsorbing microRNAs (miRNAs), thereby regulating messenger RNA (mRNA) expression. The circRNA-miRNA-mRNA regulatory network associated with type 2 diabetes mellitus (T2DM) has rarely been explored. A T2DM was established to help deepen our understanding of the molecular mechanism and therapeutic targets for T2DM.METHODS: Differentially expressed circRNAs (DEcircRNAs), miRNAs (DEmiRNAs), mRNAs (DEmRNAs) were derived from Gene...
To seek for a small interfering RNA (siRNA) sequence targeting cynomolgus monkey inhibitor of nuclear factor kappa B α (IκBα) that can specifically and effectively suppress IκBα gene expression ciliary muscle (CM) cells trabecular meshwork (TM) in vitro screen optimal siRNA transfection concentration. Three IκBα-specific double-stranded siRNAs were designed synthesized. They transfected into primarily cultured CM TM cells. The mRNA protein levels examined by using real-time quantitative...