A5057465293- Acute Myocardial Infarction Research
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Chemokine receptors and signaling
- Alzheimer's disease research and treatments
- Multiple Myeloma Research and Treatments
- Cardiac Structural Anomalies and Repair
- Cardiac Imaging and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Asthma and respiratory diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Liver physiology and pathology
- Computational Drug Discovery Methods
- CRISPR and Genetic Engineering
- Coronary Interventions and Diagnostics
- Reproductive biology and impacts on aquatic species
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Hematopoietic Stem Cell Transplantation
- T-cell and Retrovirus Studies
- Research on Leishmaniasis Studies
- Aortic Thrombus and Embolism
- S100 Proteins and Annexins
- Immune cells in cancer
- Neurotransmitter Receptor Influence on Behavior
- Immune Cell Function and Interaction
Hokkaido University
2010-2024
Nihon University
2021-2023
Nihon University Itabashi Hospital
2021
Gunma Chuo Hospital
2020
Sapporo Medical University
2013-2017
Daido University
2017
Frequency Management International (United States)
2016
Kyoto Prefectural University of Medicine
2013
University of Tsukuba
2013
Adult T-cell leukemia/lymphoma (ATLL) is one of the aggressive peripheral neoplasms with a poor prognosis. Accumulating evidence demonstrates that escape from adaptive immunity hallmark ATLL pathogenesis. However, mechanisms by which cells evade natural killer (NK)-cell-mediated have been poorly understood. Here we show CD48 expression in determines sensitivity for NK-cell-mediated cytotoxicity against cells. We performed unbiased genome-wide clustered regularly interspaced short palindromic...
Abstract Endoplasmic reticulum disulphide oxidase 1α (ERO1α) is an localized in the endoplasmic that plays a role formation of bonds secreted and cell-surface proteins. We previously showed ERO1α overexpressed various types cancer we further identified expression as novel factor related to poor prognosis cancer. However, biological functions remain unclear. Here, investigated cell roles human colon-cancer line HCT116. knockout (KO) by using CRISPR/Cas9 resulted decreased tumourigenicity vivo...
Involvement of reactive oxygen species derived from nicotinamide adenine dinucleotide phosphate, reduced form (NADPH) oxidase has been documented in the development hypoxia-induced model pulmonary arterial hypertension (PAH). Because PAH-like phenotype was demonstrated mice deficient Nox1 gene (Nox1(-/Y)) raised under normoxia, aim this study to clarify how lack NOX1/NADPH could lead pathology.Spontaneous enlargement and hypertrophy right ventricle, accompanied by vessels, were Nox1(-/Y) 9...
Alzheimer's disease (AD) differs from other forms of dementia in its relation to amyloid beta peptide (Aβ42). Using a cell culture model we previously identified annexin A5, Ca(2+), and phospholipid binding protein, as an AD biomarker. Plasma level A5 was significantly higher patients compared that control group. On the hand, has been share number clinical pathological features with Dementia Lewy bodies (DLB). The present study done examine whether or not plasma is specific marker for AD,...
X11-family proteins, including X11, X11-like (X11L) and 2 (X11L2), bind to the cytoplasmic domain of amyloid β-protein precursor (APP) regulate APP metabolism. Both X11 X11L are expressed specifically in brain, while X11L2 is ubiquitously. predominantly excitatory neurons, contrast which strongly inhibitory neurons. In vivo gene-knockout studies targeting X11L, or both, transgenic mice have reported that proteins suppress amyloidogenic processing endogenous mouse ectopic human with one...
There are a few Japanese data regarding the incidence and outcomes of acute myocardial infarction (AMI) after coronavirus disease 2019 (COVID-19) outbreak. We retrospectively reviewed AMI patients admitted to Nihon University Itabashi Hospital COVID-19 outbreak in 2020 (COVID-19 period) same period from 2017 (control period). The patients' characteristics, time course admission, diagnosis, treatment AMI, 30-day mortality were compared between two period-groups for both ST-segment elevation...
Abstract Background The clinical efficacy of the Impella for high-risk percutaneous coronary intervention (PCI) and cardiogenic shock remains under debate. We thus sought to investigate protective effects on heart with Impella’s early use pre-PCI using cardiac magnetic resonance imaging (CMRI). Methods retrospectively evaluated difference in subacute phase CMR results (19 ± 9 days after admission) between patients undergoing an (n = 7) or not (non-Impella group: n 18 [12 intra-aortic balloon...
Abstract Nicotinic acetylcholine receptors (nAChRs) in the medial habenula (MHb)–interpeduncular nucleus (IPN) pathway play critical roles nicotine-related behaviors. This is particularly enriched nAChR α3 and β4 subunits, both of which are genetically linked to nicotine dependence. However, cellular subcellular expression endogenous α3β4-containing nAChRs remains largely unknown because specific antibodies appropriate detection methods were unavailable. Here, we successfully uncovered...
Hypoxia is an important factor that contributes to tumour aggressiveness and correlates with poor prognosis resistance conventional therapy. Therefore, identifying hypoxic environments within tumours extremely useful for understanding cancer biology developing novel therapeutic strategies. Several studies have suggested carbonic anhydrase 9 (CA9) a reliable biomarker of hypoxia potential target, while pimonidazole has been identified as exogenous marker. However, other CA9 expression not...
[Figure: see text] Multiple myeloma (MM) remains an intractable hematological malignancy, despite recent advances in anti-MM drugs. Here, we show that role of PDZ binding kinase (PBK) MM tumor growth. We identified interleukin-6 (IL-6) readily increases PBK expression. Kaplan-Meier analysis showed the patients with higher expression have a significant shorter survival time compared those moderate/lower PBK. Knockout dramatically suppressed vivo growth cells, while genome editing changing...
Abstract We developed a novel and convenient method for rapidly identifying CRISPR/Cas9-based genome-edited biallelic knockout (KO) cells/individuals carrying insertions or deletions of few nucleotides (indels) by performing PCR on genomic DNA samples under stringent conditions low MgCl 2 concentrations. The KO can be judged as ‘negative’ these conditions. sense primer corresponds to the sequence recognised guide RNA subsequently cleaved Cas9 immediately upstream target gene’s proto-spacer...
Both cardiogenic shock (CS) and critical culprit lesion locations (CCLLs), defined as the left main trunk proximal anterior descending coronary artery, are associated with worse outcomes in ST-elevation myocardial infarctions (STEMIs). We aimed to examine how combination of CS and/or CCLLs affected prognosis Japanese STEMI patients primary percutaneous intervention era (PPCI-era). The subjects included 624 admitted our hospital between January 2013 April 2020. They were divided into four...
Creating genetically modified (GM) animals using CRISPR/Cas mediated through the electroporation of two-cell stage embryos, rather than fertilized eggs, holds considerable potential. The full potential genome editing embryos is only beginning to be explored. We developed an improved method prevent blastomere fusion in two-cell-stage enabling efficient editing. Using this method, we demonstrated that indel mutation rates and ssODN knock-in (KI) efficiencies are comparable those with a...
Background and aimsThe optimal duration of dual antiplatelet therapy for acute myocardial infarction is controversial because the bleeding risk outweighs thromboembolic risk. We hypothesized that an in-stent thrombus (IS-thrombus) detected by coronary angioscopy (CAS) after stent implantation would be associated with high risk.MethodsThis study included 208 patients who underwent CAS at 2 weeks infarction. The was approved ethics committee Nihon University Itabashi Hospital (reference number...