Effective drug delivery is important in the treatment of various biomedical conditions, ranging from autoimmune disorders to cancer and bacterial infections. Nanostructured systems can help overcome challenges efficient such as poor distribution, inefficient penetration across biological barriers off-target effects. The bioinspired nanotopography carrier surfaces provides a physical cue modulate their interaction with systems. In this Review, we discuss how naturally occurring...

Supramolecular self-assembly offers promising new ways to control nanostructure morphology and respond external stimuli. A pH-sensitive self-assembled system was developed both shape the acidic microenvironment of tumors using self-assembling peptide amphiphiles (PAs). By incorporating an oligo-histidine H6 sequence, we two PAs that into distinct morphologies on nanoscale, either as nanofibers or spherical micelles, based incorporation aliphatic tail N-terminus near C-terminus, respectively....

Glioblastoma is a particularly challenging cancer, as there are currently limited options for treatment. New delivery routes being explored, including direct intratumoral injection via convection-enhanced (CED). While promising, of traditional chemotherapeutics such doxorubicin (DOX) has seen success. Several studies have demonstrated that attaching drug to polymeric nanoscale materials can improve efficacy CED. We therefore set out evaluate panel morphologically distinct protein...

Azide-alkyne cycloaddition is a powerful reaction for the formation of bioconjugates. When catalyzed by Cu(I) or strain promotion, this considered to be "click" with many applications in chemical biology and materials science. We report new type azide-alkyne click chemistry synthesis protein conjugates using cucurbit[6]uril (CB6) supramolecular chemistry. CB6-promoted has been previously used rotaxanes but not applied development complex By developing substrates CB6 that do contain any...

Bacterial biofilm infections, particularly those of Pseudomonas aeruginosa (PA), have high rates antimicrobial tolerance and are commonly found in chronic wound cystic fibrosis lung infections. Combination therapeutics that act synergistically can overcome tolerance; however, the delivery multiple at relevant dosages remains a challenge. We therefore developed nanoscale drug carrier for codelivery by combining approaches from polyelectrolyte nanocomplex (NC) formation layer-by-layer...

Current approaches to nanoscale therapeutic delivery rely on the attachment of a drug interest nanomaterial scaffold that is capable releasing selectively in tumor environment. One class nanocarriers receiving significant attention protein nanomaterials, which are biodegradable and homogeneous morphology can be equipped with multiple functional handles for attachment. Although most protein-based spherical morphology, recent research has revealed nonspherical nanomaterials may have favorable...

¹²⁹Xe–cucurbit[6]uril NMR signals were blocked <italic>via</italic> rotaxanes until chemically activated. This platform is customizable for a broad range of applications.

Neutrophil extracellular traps (NETs) are networks of decondensed chromatin, histones, and antimicrobial proteins released by neutrophils in response to an infection. NET overproduction can cause exacerbated hyperinflammatory a variety diseases lead host tissue damage without clearance Nanoparticle drug delivery is promising avenue for creating materials that both target NETs deliver sustained amounts NET-degrading drugs alleviate hyperinflammation. Here, we study how particle...

We report on the design and implementation of a cucurbit[6]uril rotaxane probe for detection matrix metalloproteases by Xe hyperCEST NMR.

Introduction: Currently, there are no non-surgical FDA-approved biological approaches to accelerate fracture repair. Injectable therapies designed stimulate bone healing represent an exciting alternative surgically implanted biologics, however, the translation of effective osteoinductive remains challenging due need for safe and drug delivery. Hydrogel-based microparticle platforms may be a clinically relevant solution create controlled localized delivery treat fractures. Here, we describe...

We have synthesized targeted, selective, and highly sensitive 129Xe NMR nanoscale biosensors using a spherical MS2 viral capsid, Cryptophane A molecules, DNA aptamers. The showed strong binding specificity toward targeted lymphoma cells (Ramos line). Hyperpolarized signal contrast hyper-CEST MRI image indicated its promise as hyperpolarized biosensor for future applications in cancer detection vivo.

The development of sensitive and chemically selective MRI contrast agents is imperative for the early detection diagnosis many diseases. Conventional responsive used in

We report a (129)Xe NMR relaxation-based sensing approach that exploits changes in the bulk xenon relaxation rate induced by slowed tumbling of cryptophane-based sensor upon target binding. The amplification afforded detection dissolved allows sensitive targets. comprises xenon-binding cryptophane cage, interaction element, and metal chelating agent. Xenon associated with target-bound cage is rapidly relaxed then detected after exchange bulk. Here we show large macromolecular targets...

ABSTRACT Tight junctions regulate epithelial barrier function and have been shown to be influenced by multiple classes of proteins. Apical integrins identified as potential regulators function; however, only indirect approaches used measure integrin regulation the barrier. Here, we polymeric nanowires conjugated with anti-integrin β1 antibodies specifically target apically localized in either their closed or open conformation. Barrier apical was found conformation specific. Nanowires...

We have previously reported that low concentrations of interferon (IFN)-activated monocytes exert near-eradicative cytocidal activity against several human tumor cells in vitro. In the present study, we examined 7 cell lines and 3 diploid presence or absence 10 ng/mL IFNα2a monocytes. The results confirmed strong 4 but none lines. To model larger vivo tumors, increased target concentration determined monocytes, required for death. found increasing from 10- to 100-fold (105 cells/well) an...

Abstract Injectable colloids that self‐assemble into 3D networks are promising materials for applications in regenerative engineering, as they create open systems cellular infiltration, interaction, and activation. However, most injectable have spherical morphologies, which lack the high material‐biology contact areas afforded by higher aspect ratio materials. To address this need, particles (HARPs) developed form to enhance scaffold assembly dynamics interactions. HARPs functionalized...

Abstract The development of sensitive and chemically selective MRI contrast agents is imperative for the early detection diagnosis many diseases. Conventional responsive used in 1 H are impaired by high abundance protons body. 129 Xe hyperCEST NMR/MRI comprises a highly complement to traditional because its ability report specific chemical environments. To date, scope NMR lacks breadth small molecules, which often important markers disease. Herein, we synthesis characterization...

Polymeric particles with intricate morphologies and properties have been developed based on bioinspired designs for applications in regenerative medicine, tissue engineering, drug delivery. However, the fabrication of asymmetric functionalities remains a challenge. Janus polymeric are an emerging class material functionalities; however, they predominantly spherical morphology, made from non-biocompatible materials, using specialized techniques. We therefore set out to fabricate nonspherical...

Oral protein delivery technologies often depend on encapsulating or enclosing the cargo to protect it against pH-driven degradation in stomach enzymatic digestion small intestine. An emergent methodology is encapsulate therapeutics microscale, asymmetric, planar microparticles, referred as microdevices. Previous work has shown that, compared spherical particles, microdevices have longer residence times GI tract, but remains unclear how specific design choices (e.g., material selection,...

Abstract New approaches are needed to treat bacterial biofilm infections, particularly those of Pseudomonas aeruginosa (PA), which have high rates antimicrobial resistance and commonly found in chronic wound cystic fibrosis lung infections. Combination therapeutics that act synergistically can overcome resistance; however, the delivery multiple at relevant dosages remains a challenge. We therefore developed new nanoscale drug carrier for co-delivery by combining from polyelectrolyte...