A5067972591- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Gene expression and cancer classification
- Viral Infectious Diseases and Gene Expression in Insects
- Glioma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Herpesvirus Infections and Treatments
- Neuropeptides and Animal Physiology
- Pluripotent Stem Cells Research
- Neuroblastoma Research and Treatments
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Glycosylation and Glycoproteins Research
- Cancer, Stress, Anesthesia, and Immune Response
- Chemokine receptors and signaling
- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
Center for Biologics Evaluation and Research
2014-2023
United States Food and Drug Administration
2012-2023
Theravance Biopharma (United States)
2023
Yokohama City University
1998-2023
Duke University
2007-2016
Food and Drug Administration
1993-2016
University of North Carolina at Charlotte
2016
OncoTab (United States)
2016
Bhabha Atomic Research Centre
2013-2015
Data Harbor (United States)
2015
The interleukin-2 (IL-2) receptor γ chain (IL-2Rγ) is an essential component of high- and intermediate-affinity IL-2 receptors. IL-2Rγ was demonstrated to be a the IL-4 on basis chemical cross-linking data, ability augment binding affinity, requirement for in IL-4-mediated phosphorylation insulin substrate-1. observation that functional receptor, together with finding associates IL-7 begins elucidate why deficiency this common (gamma; c ) has profound effect lymphoid function development, as...
Convection-enhanced delivery (CED) of cintredekin besudotox (CB) was compared with Gliadel wafers (GW) in adult patients glioblastoma multiforme (GBM) at first recurrence. Patients were randomized 2:1 to receive CB or GW. (0.5 µg/mL; total flow rate 0.75 mL/h) administered over 96 hours via 2–4 intraparenchymal catheters placed after tumor resection. GW (3.85%/7.7 mg carmustine per wafer; maximum 8 wafers) immediately The primary endpoint overall survival from the time randomization....
Reproducibility is a fundamental requirement in scientific experiments. Some recent publications have claimed that microarrays are unreliable because lists of differentially expressed genes (DEGs) not reproducible similar Meanwhile, new statistical methods for identifying DEGs continue to appear the literature. The resultant variety existing and emerging exacerbates confusion continuing debate microarray community on appropriate choice reliable DEG lists. Using data sets generated by...
Glioblastoma multiforme (GBM) is a devastating brain tumor with median survival of 6 months after recurrence. Cintredekin besudotox (CB) recombinant protein consisting interleukin-13 (IL-13) and truncated form Pseudomonas exotoxin (PE38QQR). Convection-enhanced delivery (CED) locoregional-administration method leading to high-tissue concentrations large volume distributions. We assessed the use intracerebral CED deliver CB in patients recurrent malignant glioma (MG).Three phase I clinical...
We have cloned cDNAs corresponding to the human interleukin 13 receptor α chain (IL-13Rα). The protein has 76% homology murine IL-13Rα, with 95% amino acid identity in cytoplasmic domain. Only weak IL-13 binding activity was found cells transfected only IL-13Rα; however, combination of both IL-13Rα and IL-4Rα resulted substantial activity, a Kd approximately 400 pM, indicating that chains are essential components receptor. Whereas serves as an alternative accessory common cytokine γ (γc) for...
Interleukin 13 (IL-13) shares many biological properties with IL-4, and although the receptor for IL-4 (IL-4R) has been characterized, expression structure of IL-13 are unknown. We report here that human renal cell carcinoma (RCC) cells express large numbers functional IL-13R. Human B lymphocytes monocytes expressed a very small number IL-13R, while resting or activated T little no did not compete binding, competed even though IL-4R IL-13R structurally distinct on RCC cells. cross-linked one...
Convection-enhanced delivery (CED) is a novel intracerebral drug technique with considerable promise for delivering therapeutic agents throughout the CNS. Despite this promise, Phase III clinical trials employing CED have failed to meet end points. Although may be due inactive or failure rigorously validate targets, authors previously demonstrated that catheter positioning plays major role in distribution using technique. The purpose of present work was retrospectively analyze expected based...
Chimeric proteins provide a unique opportunity to target therapeutic bacterial toxins subset of specific cells. We have generated new recombinant chimeric toxin composed human interleukin 13 (hIL13) and Pseudomonas exotoxin A (PE) mutant, PE38QQR. The hIL13-PE38QQR chimera is highly cytotoxic cell lines derived from several epithelial carcinomas such as adenocarcinoma stomach, colon, skin. action hIL13-PE38QQR, which can only occur upon internalization ligand-receptor complex, blocked by an...
Abstract Background The acceptance of microarray technology in regulatory decision-making is being challenged by the existence various platforms and data analysis methods. A recent report (E. Marshall, Science , 306, 630–631, 2004), extensively citing study Tan et al . ( Nucleic Acids Res ., 31, 5676–5684, 2003), portrays a disturbingly negative picture cross-platform comparability, and, hence, reliability technology. Results We reanalyzed Tan's dataset found that intra-platform consistency...
Two 8 S forms of progesterone receptor from the chicken oviduct were purified to near homogeneity and analyzed for peptide composition by gel electrophoresis.Form I contains two major peptides with molecular weights 90,000 75,000.Form I1 also 110,000.In glycerol gradients containing molybdate, 90,000, 110,000, 75,000 weight co-sediment [3H]progesterone peak at S. In high salt lacking co-sediments 110,000 4 S, while sediments 6-7 On photoactivation, synthetic progestin R5020 binds covalently...
Previously, Puri et al. (Puri, R. K., M. Ogata, P. Leland, G. Feldman, D. Fitzgerald, and I. Pastan. 1991. Cancer Res. 51:3011-3017) have demonstrated that murine sarcoma colon adenocarcinoma cells express high affinity interleukin-4 receptors (IL-4R) which are internalized after binding to a chimeric ligand consisting of IL-4 Pseudomonas exotoxin. In the present study, we tested primary cultures human renal cell carcinoma (RCC) cells, generated from tumor specimens obtained nephrectomy, for...
Previously, we have demonstrated that a variety of human cancers including the ovarian cancer express IL-13Rα2, high affinity receptor for IL-13. Herein, examined if IL-13 regulates invasion and metastasis through IL-13Rα2 in vitro vivo animal models cancer. We tested cell protease activity IL-13Rα2-overexpressing IL-13Rα2-negative tumor lines. treatment significantly augmented both enzyme activities only IL-13Rα2-positive cells but not vitro. Mechanistically, enhanced ERK1/2, AP-1 MMP...