A5067007394- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Neurogenesis and neuroplasticity mechanisms
- Congenital heart defects research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Biomedical Text Mining and Ontologies
- Sphingolipid Metabolism and Signaling
- Transgenic Plants and Applications
- Hedgehog Signaling Pathway Studies
- RNA and protein synthesis mechanisms
- Childhood Cancer Survivors' Quality of Life
- RNA modifications and cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hypothalamic control of reproductive hormones
- Nuclear Receptors and Signaling
- Cellular transport and secretion
- Genetic Syndromes and Imprinting
- Semantic Web and Ontologies
- Genetics, Aging, and Longevity in Model Organisms
- Nerve injury and regeneration
University of Georgia
2012-2024
Augusta University Health
2010
Paul Hastings
2010
Augusta University
1999-2005
Center for Biologics Evaluation and Research
2004
National Institute on Aging
2004
Geron (United States)
2004
Rambam Health Care Campus
2004
Menlo School
2004
University of Illinois Chicago
2001
The use of human embryonic stem cells (hESCs) as a source dopaminergic neurons for Parkinson's disease cell therapy will require the development simple and reliable differentiation protocols. cocultures, added extracellular signaling factors, or transgenic approaches to drive hESC could lead additional regulatory well production delays these therapies. Because neuronal lineage seems limited no its formation, we tested ability hESCs differentiate form dopamine‐producing in serum‐free...
Gene targeting in embryo-derived stem (ES) cells was used to generate mice with a disruption the homeobox-containing gene Hoxd-3 (Hox-4.1). Mice homozygous for this mutation show radically remodeled craniocervical joint. The anterior arch of atlas is transformed an extension basioccipital bone skull. lateral masses also assume morphology more closely resembling exoccipitals and, variable extent, fuse exoccipitals. Formation second cervical vertebra, axis, affected. dens and superior facets...
Heterozygous reeler mice (HRM) haploinsufficient for reelin express ≈50% of the brain content wild-type mice, but are phenotypically different from both and homozygous mice. They exhibit, ( i ) a down-regulation glutamic acid decarboxylase 67 (GAD )-positive neurons in some not every cortical layer frontoparietal cortex (FPC), ii an increase neuronal packing density decrease thickness because neuropil hypoplasia, iii dendritic spine expression on basal apical branches motor FPC III pyramidal...
Transformation of epithelial cells is associated with loss cell polarity, which includes alterations in morphology as well changes the complement plasma membrane proteins. Rab proteins regulate polarized trafficking to and therefore represent potential regulators this neoplastic transition. Here we have demonstrated a tumor suppressor function for Rab25 intestinal neoplasia both mice humans. Human colorectal adenocarcinomas exhibited reductions expression independent stage, lower levels...
The formation of stem cell–derived tumors (teratomas) is observed when engrafting undifferentiated embryonic (ES) cells, embryoid body–derived cells (EBCs), or mammalian embryos and a significant obstacle to cell therapy. We show that in formed after engraftment EBCs into mouse brain, expression the pluripotency marker Oct-4 colocalized with prostate apoptosis response-4 (PAR-4), protein mediating ceramide-induced during neural differentiation ES cells. tested ability novel ceramide analogue...
We have developed a culture system for the efficient and directed differentiation of human embryonic stem cells (HESCs) to neural precursors neurons.HESC were maintained by manual passaging differentiated morphologically distinct OCT-4+/SSEA-4- monolayer cell type prior derivation embryoid bodies. Embryoid bodies grown in suspension serum free conditions, presence 50% conditioned medium from hepatocarcinoma line HepG2 (MedII).A precursor population was observed within HESC derived cultured...
Human ES (hES) cell lines have only recently been generated, and differences between human mouse cells identified. In this manuscript we describe the properties of two lines, BG01 BG02. By immunocytochemistry reverse transcription polymerase chain reaction, undifferentiated expressed markers that are characteristic cells, including SSEA‐3, SSEA‐4, TRA‐1‐60, TRA‐1‐81, OCT‐3/4. Both were readily maintained in an state could differentiate into all three germ layers, as determined by expression...
The functions of neurotransmitters in fetal development are poorly understood. Genetic observations have suggested a role for the inhibitory amino acid neurotransmitter γ-aminobutyric (GABA) normal mouse palate. Mice homozygous mutations β-3 GABA A receptor subunit develop cleft secondary GABA, ligand this receptor, is synthesized by enzyme glutamic decarboxylase. We disrupted one two Gad genes gene targeting and also find defects formation striking similarity phenotype between clearly...
Heterozygous mutations of GATA3, which encodes a dual zinc-finger transcription factor, cause hypoparathyroidism with sensorineural deafness and renal dysplasia. Here, we have investigated the role GATA3 in parathyroid function by challenging Gata3+/- mice diet low calcium vitamin D so as to expose any defects function. This led higher mortality among compared Gata3+/+ mice. Compared their wild-type littermates, had lower plasma concentrations hormone (PTH) smaller glands reduced Ki-67...
Abstract Background Thymic epithelial cells (TECs) promote thymocyte maturation and are required for the early stages of development positive selection. However, investigation mechanisms by which TECs perform these functions has been inhibited lack genetic tools. Since Foxn1 gene is expressed in all presumptive from thymus organogenesis broadly adult thymus, it an ideal locus driving expression differentiating mature TECs. Results We generated two knock-in alleles inserting IRES-Cre or...
ABSTRACT The homeobox containing transcript Xhox-36 is expressed exclusively in the posterior mesoderm and ectoderm of early Xenopus embryos. Therefore, shows region-specific rather than tissuespecific expression gastrula neurula, a time when cells are becoming committed to defined fates. Exposure embryos LiCl, which shifts more anterior fates, reduces abundance this posterior-specific transcript. In contrast, ventralized by u.v. treatment express normal levels transcript, implying that gene...
Cell death and survival of neural progenitor (NP) cells are determined by signals that largely unknown. We have analyzed pro-apoptotic signaling in individual NP been derived from mouse embryonic stem cells. formation was concomitant with elevated apoptosis increased expression ceramide prostate response 4 (PAR-4). Morpholino oligonucleotide-mediated antisense knockdown PAR-4 or inhibition biosynthesis reduced cell apoptosis, whereas overexpression treatment analogs apoptosis. Apoptotic also...
The transcription factor FOXN1 is essential for fetal thymic epithelial cell (TEC) differentiation and proliferation. Postnatally, Foxn1 levels vary widely between TEC subsets, from low/undetectable in putative progenitors to highest differentiated subsets. Correct expression required maintain the postnatal microenvironment; premature downregulation of causes a rapid involution-like phenotype, transgenic overexpression can cause hyperplasia and/or delayed involution. We investigated K5.Foxn1...
Stromal derived growth factor (SDF-1) and gamma-aminobutyric acid (GABA) are two extracellular cues that regulate the rate of neuronal migration during development may act synergistically. The molecular mechanisms this interaction still unclear. Gonadotropin releasing hormone-1 (GnRH) neurons essential for vertebrate reproduction. During development, these emerge from nasal placode migrate through cribriform plate into brain. Both SDF-1 GABA have been shown to GnRH by accelerating slowing...
Neuronal activity elicits a rapid increase in the expression of several immediate early genes (IEGs). To clarify role for IEG response activity-dependent development, we examined contribution egr1/zif268 gene during visual cortical processing and plasticity mice. We first analyzed egr1 mRNA wild-type (WT) mice using Northern blot hybridization. In cortex, increased dramatically after eye opening, systemic injection kainate, or 30 min photostimulation brief (5 d) period dark adaptation. Thus,...
The GABA-synthesizing enzyme glutamic acid decarboxylase (GAD) is expressed in pancreatic beta-cells and GABA has been suggested to play a role islet cell development function. Mouse predominantly express the larger isoform of enzyme, GAD67, very low levels second isoform, GAD65. Yet GAD65 shown be target early autoimmune T-cell responses associated with beta-cell destruction non-obese diabetic (NOD) mouse model Type 1 diabetes. Mice deficient or both were used assess whether important for...