A5046174479- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Nerve Injury and Rehabilitation
- Axon Guidance and Neuronal Signaling
- Suicide and Self-Harm Studies
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- COVID-19 and Mental Health
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Anesthesia and Neurotoxicity Research
- Bone Tissue Engineering Materials
- Spine and Intervertebral Disc Pathology
- Neurological Disorders and Treatments
- Signaling Pathways in Disease
- Tendon Structure and Treatment
- Hypothalamic control of reproductive hormones
- Herpesvirus Infections and Treatments
- Graphene and Nanomaterials Applications
- Muscle Physiology and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Surgical Sutures and Adhesives
- RNA Interference and Gene Delivery
- Traumatic Brain Injury and Neurovascular Disturbances
University of Naples Federico II
2022-2025
Federico II University Hospital
2021-2024
Neuroscience Institute
2009-2022
Midwestern University
2011-2022
Casa di Cura San Michele
2020
University of Turin
2003-2015
University of Catania
2015
National Institutes of Health
2011-2012
National Institute of Neurological Disorders and Stroke
2011-2012
Ospedale San Luigi Gonzaga
2001-2011
Cachexia is a wasting syndrome associated with cancer, AIDS, multiple sclerosis, and several other disease states. It characterized by weight loss, fatigue, loss of appetite, skeletal muscle atrophy poor patient prognosis, making it an important treatment target. Ghrelin peptide hormone that stimulates growth (GH) release positive energy balance through binding to the receptor GHSR-1a. Only acylated ghrelin (AG), but not unacylated form (UnAG), can bind GHSR-1a; however, UnAG AG share...
Abstract Whether post‐traumatic regeneration can eventually result in rat peripheral nerve fibers regaining their pretrauma size is still an open question. While it has been shown that, after a sufficient duration time, the number of regenerated return to numbers and animal regain normal prelesion function, no information regarding long‐term changes parameters available. To fill this gap, we have investigated myelinated axon fiber diameter, myelin thickness, g ‐ratio (the ratio inner axonal...
Stromal derived growth factor (SDF-1) and gamma-aminobutyric acid (GABA) are two extracellular cues that regulate the rate of neuronal migration during development may act synergistically. The molecular mechanisms this interaction still unclear. Gonadotropin releasing hormone-1 (GnRH) neurons essential for vertebrate reproduction. During development, these emerge from nasal placode migrate through cribriform plate into brain. Both SDF-1 GABA have been shown to GnRH by accelerating slowing...
Abstract Over the last 10 years, we have investigated a particular type of bioengineered nerve guide, muscle‐vein‐combined tube, which is made by filling vein with skeletal muscle. In our previous studies always used fresh muscle to fill conduits. present study compared use and predegenerated (freeze‐thawed) for guides. this study, 10‐mm‐long rat median defect was repaired using either guide. The samples were analyzed 5 30 days after surgery light electron microscopy. addition, reverse...
Abstract Peripheral nerves possess the capacity of self-regeneration after traumatic injury but extent regeneration is often poor and may benefit from exogenous factors that enhance growth. The use cellular systems a rational approach for delivering neurotrophic at nerve lesion site, in present study we investigated effects enwrapping site end-to-end rat sciatic repair with an equine type III collagen membrane enriched or not N1E-115 pre-differentiated neural cells. After neurotmesis, was...
The evidence of neurons generated ex novo in sensory ganglia adult animals is still debated. In the present study, we investigated, using high resolution light microscopy and stereological analysis, changes number dorsal root after 30 days from a crush lesion rat brachial plexus terminal branches. Results showed, as expected, relevant hypertrophy ganglion neurons. addition, reported, for first time literature, that neuronal was accompanied by massive hyperplasia leading to 42% increase...
Abstract Schwann cells guide axonal regrowth during peripheral nerve repair. In a case of lesion with substance loss, graft conduit is necessary to enable axons reach the distal stump. If non‐nervous autograft used, question arises as presence and origin along grafted tube. We addressed this issue using tubulization technique based on use an autologous vein filled fresh skeletal muscle for repair sciatic defects in rat. showed that both ends were early progressively colonized by number glial...
Langerhans cells (LC) represent a well characterized subset of dendritic located in the epidermis skin and mucosae. In vivo, they originate from resident blood-borne precursors presence keratinocyte-derived TGFβ. Ιn vitro, LC can be generated monocytes GM-CSF, IL-4 However, signals that induce during an inflammatory reaction are not fully investigated. Here we report Activin A, TGFβ family member induced by pro-inflammatory cytokines involved morphogenesis wound healing, induces...
Gonadotropin-releasing hormone-1 (GnRH-1) neurons migrate from the nasal placode to forebrain where they control gonadal function via hypothalamic–pituitary–gonadal axis. The birth of GnRH-1-expressing is one first neurogenic events in developing placode. By gene expression screening on single GnRH-1 neurons, amyloid precursor binding protein-1 (FE65) was identified migratory neurons. FE65 has been shown modulate β1-integrin dynamics, actin cytoskeleton, cell motility, and FE65/amyloid...
Among the various grafting procedures that have been studied as alternatives to traditional fresh nerve autografts for repair of severed peripheral nerves, muscle-vein-combined graft conduits recently devised and successfully employed. In present study, early presence, origin, proliferation activity Schwann cells (SCs) along this particular type biological conduit investigated, using antibodies directed against glial fibrillar acid protein (GFAP), a is specifically expressed in cells,...
This protocol describes an ex vivo model of mouse-derived dorsal root ganglia (DRG) explant and in vitro DRG-derived co-culture dissociated sensory neurons glial satellite cells.These are useful versatile models to investigate a variety biological responses associated with physiological pathological conditions the peripheral nervous system (PNS) ranging from neuron-glial interaction, neuroplasticity, neuroinflammation, viral infection.The usage DRG is scientifically advantageous compared...