A5062893022- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Oral and Maxillofacial Pathology
- Enzyme Catalysis and Immobilization
- Nerve injury and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Plant biochemistry and biosynthesis
- Craniofacial Disorders and Treatments
- Cleft Lip and Palate Research
- Salivary Gland Tumors Diagnosis and Treatment
- Microbial Metabolic Engineering and Bioproduction
- Cell Adhesion Molecules Research
- Parathyroid Disorders and Treatments
- Polysaccharides and Plant Cell Walls
- Amino Acid Enzymes and Metabolism
- Cerebrovascular and Carotid Artery Diseases
- RNA Interference and Gene Delivery
- Plant Surface Properties and Treatments
- Peroxisome Proliferator-Activated Receptors
- Metabolism and Genetic Disorders
- Head and Neck Surgical Oncology
- Tracheal and airway disorders
- Neuroscience and Neuropharmacology Research
- Metabolomics and Mass Spectrometry Studies
- MRI in cancer diagnosis
Tokyo University of Technology
2025
Tohoku University
1982-2025
Tenri Hospital
2019-2024
Shizuoka City Hospital
2021-2024
Itami City Hospital
2024
Mi Corporation (United States)
2024
Gifu University Hospital
2022-2024
Hokkaido University
2024
Tenri University
2023
Cambridge University Press
2022
The Keap1-Nrf2 system plays a central role in cytoprotection against electrophilic/oxidative stresses. Although Cys151, Cys273, and Cys288 of Keap1 are major sensor cysteine residues for detecting these stresses, it has not been technically feasible to evaluate the functionality Cys273 or Cys288, since mutants that harbor substitutions maintain ability repress Nrf2 accumulation do exist. To overcome this problem, we systematically introduced amino acid into Cys273/Cys288 finally identified...
Neurotrophic support to peripheral sensory neurons is provided by 2 factors of related sequence, NGF and brain-derived neurotrophic factor (BDNF). present in target tissues, while BDNF has only been reported the CNS. We now report biological characterization molecular cloning a cDNA for from human platelets. platelets activities very similar those obtained adult porcine brain neuron- enriched cultures prepared ganglia chick embryos at 8-12 d incubation. promoted survival neurite outgrowth...
Once brain ischemia was induced in the gerbil cerebral fronto-parietal cortex, serial changes occurred energy metabolites and various lipids. The amounts of inositol-containing phospholipids began to decrease immediately after failure, followed by an increase amount 1,2-diacylglycerol with a subsequent liberation arachidonic acid other free fatty acids. compositions phospholipids, 1,2-diacylglycerols produced ischemia, acids liberated during were quite similar. stearic much larger than that...
Ceramide has emerged as a novel lipid mediator in cell proliferation, differentiation, and apoptosis. In this work, we demonstrate that the levels of c-jun mRNA, c-Jun protein, DNA binding activity nuclear transcription factor AP-1 to 12-o-tetradecanoylphorbol 13-acetate responsive elements all increased following treatment with cell-permeable ceramide, N-acetylsphingosine human leukemia HL-60 cells. N-Acetylsphingosine (1-10 μM) mRNA dose-dependent manner, maximal expression was achieved 1...
Ciliary neurotrophic factor (CNTF) and basic fibroblast growth (bFGF) were tested for effects on sprouting by motor neurons innervating the adult mouse gluteus muscle. Factors delivered subcutaneous injection directly over surface of superior muscle once daily 7 d then end plates axons visualized combined silver cholinesterase staining. CNTF (500 ng daily) induced both from subset nodes Ranvier that are closest to plate. The effect was potentiated twofold coadministration bFGF at doses 2-20...
Ganglioside GM3 inhibits epidermal growth factor (EGF)-dependent cell proliferation in a variety of lines. Both vitro and vivo, this glycosphingolipid the kinase activity EGF receptor (EGFR). Furthermore, membrane preparations containing EGFR can bind to GM3-coated surfaces. These data suggest that may interact directly with EGFR. In study, interaction gangliosides extracellular domain (ECD) was investigated. The purified human recombinant ECD from insect cells bound ganglioside GM3. site...
The chemokine receptors possess seven‐transmembrane helices connected by an extracellular N‐terminal region, three loops (ECL1‐3), intracellular loops, and C‐terminal region. We have developed specific monoclonal antibodies (mAbs) against for flow cytometry using the Cell-Based Immunization Screening (CBIS) N-terminal peptide immunization methods. However, there are few reports establishing anti-chemokine receptor mAbs of ECL peptides. Here, we established anti-mouse CCR7 (mCCR7) mAb,...
Podoplanin (PDPN) is a highly glycosylated type I transmembrane protein. PDPN expression observed in various normal tissues, including lymphatic endothelial cells, kidney podocytes, and alveolar epithelial cells the lungs. Monoclonal antibodies (mAbs) targeting across different animal species have facilitated identification of PDPN-positive cells. To date, we developed anti-PDPN mAbs for over 20 species. These suit applications, flow cytometry, immunoblotting, immunohistochemistry. In this...
Ganglioside GM3 is a membrane component that has been described to modulate cell growth through inhibition of EGF receptor associated tyrosine kinase. In order determine if the by this ganglioside specifically mediated signaling, effects on key enzymes implicated in signaling were determined and compared another inhibitor Treatment A1S cells culture or kinase inhibitor, leflunomide, led MAP PI3 activities. There was no detectable effect phosphotyrosine phosphatases. free system, however, had...
N-linked oligosaccharides appear to be important for the function of epidermal growth factor (EGF) receptor. In a previous study (Rebbaa, A., Yamamoto, H., Moskal, J. R., and Bremer, E. G. (1996) Neurochem. 67, 2265-2272), we showed that binding erythroagglutinating phytohemagglutin lectin from Phaseolus vulgaris bisecting structures on EGF receptor U373 MG glioma cells blocked autophosphorylation. this examined consequences overexpression structure by gene transfection with...
Abstract Glutamine synthetase (GS) isolated from human brain formed a single band on sodium dodecyl sulfate‐poly‐acrylamide gel with molecular weight of 44,000. The enzyme had specific activity 179.2 U/mg protein when assayed by measuring the rate formation γ‐glutamylhydroxamate using hydroxylamine as substrate. In presence manganese ions, relative GS was much lower than that sheep enzyme. suppression increasing ADP concentration, however, less marked in Antibodies were raised rabbits...
Iron supplementation is a mainstay for management of renal anaemia in patients receiving haemodialysis (HD). Although it well known that single intravenous iron (IVIR) administration transiently enhances oxidative stress HD patients, the consequence repeated IVIR still unknown. This study aims to clarify time course changes serum 8-hydroxy-2'-deoxyguanosine (8-OHdG), marker DNA injury, during period patients.Twenty-seven (62+/-14 years and 23 males) on long-term participated this study. All...