We aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using the endophytic fungal extract of Aspergillus niger. The prepared ZnO NPs were characterized, and their in vitro vivo antibacterial activity was investigated. Isolated fungus identification carried out 18S rRNA. A. niger employed for green synthesis NPs. elucidated against Staphylococcus aureus broth microdilution method quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, a systemic infection model mice....

Abstract Carbapenem-resistant Klebsiella pneumoniae poses a severe risk to global public health, necessitating the immediate development of novel therapeutic strategies. The current study aimed investigate effectiveness green algae Arthrospira maxima (commercially known as Spirulina ) both in vitro and vivo against carbapenem-resistant K. . In this study, thirty isolates were collected, identified, then screened for their susceptibility several antibiotics carbapenemase production genes...

can cause a wide range of severe infections owing to its multiple virulence factors in addition resistance antimicrobials; therefore, novel antimicrobials are needed. Herein, we used

Methotrexate (MTX), an antineoplastic and immunosuppressive drug, widely used in the treatment of different types cancers management chronic inflammatory diseases. However, its use is associated with hepatotoxicity. Vitamin C (VC) curcumin (CUR) exhibit anti-inflammatory antioxidant effects. Thus, we aimed to investigate potential hepatoprotective effects VC CUR pretreatment alone combination against MTX-induced Albino mice were randomly divided into 7 groups: control group, which received...

The acute inflammation process is explained by numerous hypotheses, including oxidative stress, enzyme stimulation, and the generation of pro-inflammatory cytokines. anti-inflammatory activity Yucca gigantea methanol extract (YGME) against carrageenan-induced possible underlying mechanisms was investigated. phytochemical profile, cytotoxic, antimicrobial activities were also explored. LC-MS/MS utilized to investigate chemical composition YGME, 29 compounds tentatively identified. In...

Purpose: Gentiopicroside (GPS), an adequate bioactive candidate, has a promising approach for enhancing wound healing due to its antioxidant and antimicrobial properties. Its poor aqueous solubility negatively affects oral absorption accompanied by low bioavailability intestinal/hepatic first-pass metabolism. Our aim in this study is fabricate GPS into appropriate nanocarriers (PLGA nanospheres, NSs) enhance hence would be improved. Methods: Normal ODS silica gel together with Sephadex LH20...

The increasing emergence of bacterial resistance is a challenge for the research community, thus novel antibacterial agents should be developed. Metal nanoparticles are promising and could solve problem antibiotic resistance. Herein, we used Gardenia thailandica methanol extract (GTME) to biogenically synthesise zinc oxide (ZnO-NPs). characterisation ZnO-NPs was performed by UV spectroscopy, FTIR, scanning transmission electron microscopes, dynamic light scattering, X-ray diffraction....

Nephrotoxicity (NT) is a renal-specific situation caused by different toxins and drugs like non-steroidal anti-inflammatory (NSAIDs). NSAIDs diclofenac (DCF) lead to glomerular dysfunction. Pentoxifylline (PTX) berberine (BER) have antioxidant properties. Thus, the objective of present study was investigate ameliorative effect PTX, BER their combination against DCF-mediated acute NT. Induction NT done via DCF injection (150 mg/kg I.P, for 6 days) in rats. PTX 200 mg/kg, were administrated...

Enterococcus species possess many virulence factors that have an essential role in exacerbating the infections caused by them. The current study aimed to evaluate effect of secondary metabolites ginkgetin (GINK) and sotetsuflavone (SOTE), isolated from Cycas media R. Br dichloromethane fraction, on faecalis (E. faecalis) isolates for first time. antibacterial antivirulence activities compounds were investigated using docking studies vitro determination minimum inhibitory concentrations...

Owing to the disseminating resistance among pathogenic bacteria, especially Klebsiella pneumoniae, there is a high need for alternate compounds with antibacterial activity. Herein, lycopene was isolated from Lycopersicon esculentum L. Molecular docking approach employed explore binding affinity selected vital proteins of K. pneumoniae mechanisms being investigated. This proposed promising activity lycopene. However, pharmacological use hampered by its poor solubility and limited oral...

Abstract Pseudomonas aeruginosa is an opportunistic bacterium causing several health problems and having many virulence factors like biofilm formation on different surfaces. There a significant need to develop new antimicrobials due the spreading resistance commonly used antibiotics, partly attributed formation. Consequently, this study aimed investigate anti-biofilm anti-quorum sensing activities of Dioon spinulosum, Dyer Ex Eichler extract (DSE), against clinical isolates. DSE exhibited...

Numerous efforts to manage acute kidney injury (AKI) were unsuccessful because its pathophysiology is still poorly understood. Thus, our research hotspot was explore the possible renoprotective effects of rosuvastatin (Ros) and diosmetin (D) on macrophage polarization role HSP70/TLR4/MyD88/NF-κB p65/NLRP3/STAT3 signaling in cis-induced AKI study activity D against uropathogenic bacteria. Fifty-four albino male rats randomized into 9 groups equally: Control, Ros, D20, D40, untreated Cis, Cis...

A wound is a complicated bioprocess resulting in significant tissue damage, which worsened by secondary bacterial infection, commonly

Doxorubicin (DOX) is an anticancer agent for treating solid and soft tissue malignancies. However, the clinical use of DOX restricted by cumulative, dose-dependent cardiotoxicity. Therefore, present study aimed to assess cardioprotective effects

Candida albicans is a major human opportunistic pathogen causing infections, which range from cutaneous to invasive systemic infections. Herein, the antifungal and anti-biofilm potential of silver nanoparticles (AgNPs) green synthesized in presence Encephalartos laurentianus leaf extract (ELLE) were investigated. The bioactive chemicals ELLE, including phenolics, flavonoids, glycosides identified quantified for first time. AgNPs showed minimum inhibitory concentration (MIC) values against C....

Monterey cypress (Cupressus macrocarpa) is a decorative plant; however, it possesses various pharmacological activities. Therefore, we explored the phytochemical profile of C. macrocarpa root methanol extract (CRME) for first time. Moreover, investigated its antidiarrheal (in vivo), antibacterial, and antibiofilm vitro) activities against Salmonella enterica clinical isolates. The LC-ESI-MS/MS analysis CRME detected presence 39 compounds, besides isolation 2,3,2″,3″-tetrahydro-4'-O-methyl...

Epigallocatechin-3-gallate (EGCG) was isolated from Cycas thouarsii leaves for the first time and encapsulated in aqueous core poly(lactide-co-glycolide) (PLGA) nanocapsules (NCs). This work investigates antimicrobial activity vivo reno-protective effects of EGCG-PLGA NCs cisplatin-induced nephrotoxicity. A double emulsion solvent evaporation process adopted to prepare PLGA loaded with EGCG. Particle size, polydispersity index (PDI), zeta potential, percent entrapment efficiency (%EE),...

Non-alcoholic fatty liver disease (NAFLD) is a common chronic metabolic affecting millions globally. This study aimed to assess the safety and efficacy of high oral loading dose cholecalciferol supplement on NAFLD patients investigate its potential role serum inflammatory biomarkers.One hundred with type 2 diabetes mellitus were involved in study. Eligible randomized among two equal groups. Group 1 received standard conventional therapy addition placebo. plus for 4 months. The improvement...