A5007733997- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Gastroesophageal reflux and treatments
- Eosinophilic Esophagitis
- Estrogen and related hormone effects
- Helicobacter pylori-related gastroenterology studies
- Ion Channels and Receptors
- Inflammatory Bowel Disease
- Gut microbiota and health
- Neuropeptides and Animal Physiology
- Liver Disease Diagnosis and Treatment
- Eicosanoids and Hypertension Pharmacology
- Beetle Biology and Toxicology Studies
- Infant Nutrition and Health
- Histone Deacetylase Inhibitors Research
- Respiratory and Cough-Related Research
- Olfactory and Sensory Function Studies
- Pharmaceutical studies and practices
- Sarcoidosis and Beryllium Toxicity Research
- Infant Health and Development
- Barrier Structure and Function Studies
- Pancreatic and Hepatic Oncology Research
- IL-33, ST2, and ILC Pathways
- Antioxidants, Aging, Portulaca oleracea
- Receptor Mechanisms and Signaling
University of Antwerp
2015-2023
Georgetown University Medical Center
2020-2023
Georgetown University
2020-2023
Johns Hopkins Medicine
2022
Johns Hopkins University
2022
Objectives Experiments using P2X3 knock-out mice or more general P2X receptor antagonists suggest that receptors contribute to visceral hypersensitivity. We aimed investigate the effect of selective antagonist A-317491 on sensitivity under physiological conditions, during acute colitis and in post-inflammatory phase colitis. Methods Trinitrobenzene sulphonic-acid was monitored by colonoscopy: day 3 confirm presence then every 4 days, starting from 10, monitor convalescence determine exact...
Background and Purpose Serine proteases have been re suggested as important mediators of visceral pain. We investigated their effect by using newly developed serine protease inhibitors with a well‐characterized inhibitory profile in rat model post‐inflammatory irritable bowel syndrome (IBS). Experimental Approach Colitis was induced rats receiving intrarectal trinitrobenzenesulphonic acid; controls received 0.9% NaCl. Colonoscopies were performed on day 3, to confirm colitis, later until...
We aimed to identify mucin-microbiome signatures shaping the tumor microenvironment in gastric adenocarcinomas and clinical outcomes.We performed high-throughput profiling of mucin phenotypes present 108 20 functional dyspepsia cases using validated mucin-based RT-qPCRs with subsequent immunohistochemistry validation correlated data outcome parameters. The microbiota was assessed by 16S rRNA gene sequencing, taxonomy, community composition determined, microbial networks analyzed, metagenome...
Abstract Background Resistance to endocrine therapy in estrogen receptor–positive (ER+) breast cancer remains a significant clinical problem. Riluzole is FDA-approved for the treatment of amyotrophic lateral sclerosis. A benzothiazole-based glutamate release inhibitor with several context-dependent mechanism(s) action, riluzole has shown antitumor activity multiple malignancies, including melanoma, glioblastoma, and cancer. We previously reported that acquisition tamoxifen resistance...
Abstract Background Visceral hypersensitivity, an important cause of abdominal pain in disorders such as IBD and IBS, presents with a poorly understood pathophysiology limited treatment options. Several members the Mas‐related G protein‐coupled receptor family (Mrgprs) have become promising targets research. The potential link between murine Mrgpr C11 (Mrgprc11) gut nociception is currently uninvestigated. Therefore, we explored expression functional role Mrgprc11 nociceptive innervation....
Background Irritable bowel syndrome (IBS) is a chronic gastrointestinal disorder for which no diagnostic tools are currently available. Patients diagnosed using the Rome IV criteria and subtyped into diarrhea, constipation, or mixed phenotype based on their dominant stool pattern. A recent development in biomarker area analysis of volatile organic compounds (VOCs). The aim this study was to evaluate potential VOCs as phenotypic biomarkers IBS breath fecal samples. Materials methods Breath...
Dysregulation of the protease–antiprotease balance in gastrointestinal tract has been suggested as a mechanism underlying visceral hypersensitivity conditions such inflammatory bowel disease (IBD) and irritable syndrome (IBS). We aimed to study potential therapeutic role an intracolonically administered serine protease inhibitor for treatment abdominal pain post-inflammatory rat model IBS. An enema containing 2,4,6-trinitrobenzene sulfonic acid (TNBS) was used induce colitis male...
The protease activity in inflammatory bowel disease (IBD) and irritable syndrome has been studied extensively using synthetic fluorogenic substrates targeting specific sets of proteases. We explored activities colonic tissue from a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model by investigating the cleavage bioactive peptides. Pure trypsin- elastase-like proteases on one hand rats with TNBS-induced acute or post-inflammatory phase other, were incubated relevant peptides...
Afferent nerves not only convey information concerning normal physiology, but also signal disturbed homeostasis and pathophysiological processes of the different organ systems from periphery towards central nervous system. As such, increased activity or 'sensitization' mesenteric afferent has been allocated an important role in pathophysiology visceral hypersensitivity abdominal pain syndromes. Mesenteric nerve can be measured vitro isolated intestinal segment that is mounted a purpose-built...
Background: A protease/antiprotease disbalance is observed in inflammatory bowel diseases (IBD). We therefore studied the effect of novel serine protease inhibitor UAMC-00050 on intestinal inflammation and permeability a chronic colitis T cell transfer mouse model to get further insight into regulation cell-mediated immunopathology. Methods: Colitis was induced severe combined immunodeficient (SCID) mice, by adoptive CD4 + CD25 − CD62L cells. Animals were treated intraperitoneally (i.p.)...
Background: Serine proteases are believed to play a key role in the origin of abdominal pain IBD and IBS. We previously demonstrated reduction visceral post-inflammatory IBS rat model after single intraperitoneal or intracolonic administration serine protease inhibitor. The aim this study was investigate efficacy inhibition on two different animal models involving colonic insult based either acute inflammation neonatal irritation. Moreover, profiling explored colitis model. Methods: An...
Animals involved in common laboratory procedures experience minor levels of stress. The direct effect limited amounts stress on gastrointestinal function has not been reported yet. Therefore, this study aimed to assess the single-day and multi-day orogastric gavages gut physiology mice. To end, 12-wk-old female C57Bl6/J mice were randomized receive treatment with sterile water (200 µL) delivered by twice daily for a total 1 or 10 day(s). Control animals did any treatment. Subsequently, was...
Afferent nerves not only convey information concerning normal physiology, but also signal disturbed homeostasis and pathophysiological processes of the different organ systems from periphery towards central nervous system. As such, increased activity or 'sensitization' mesenteric afferent has been allocated an important role in pathophysiology visceral hypersensitivity abdominal pain syndromes. Mesenteric nerve can be measured vitro isolated intestinal segment that is mounted a purpose-built...
Abstract Background Resistance to endocrine therapy in estrogen receptor-positive (ER+) breast cancer remains a significant clinical problem. Riluzole is FDA-approved for the treatment of amyotrophic lateral sclerosis. A benzothiazole-based glutamate release inhibitor with several context-dependent mechanism(s) action, has shown anti-tumor activity multiple malignancies, including melanoma, glioblastoma, and cancer. We previously reported that acquisition Tamoxifen resistance cellular model...