A5069420044- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Carbon and Quantum Dots Applications
- Advanced Fluorescence Microscopy Techniques
- Advanced Nanomaterials in Catalysis
- Luminescence and Fluorescent Materials
- Nanocluster Synthesis and Applications
- Biosensors and Analytical Detection
- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Supramolecular Self-Assembly in Materials
- Quantum Dots Synthesis And Properties
- Alzheimer's disease research and treatments
- Advanced Biosensing Techniques and Applications
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Plasmonic and Surface Plasmon Research
- Near-Field Optical Microscopy
- Cholinesterase and Neurodegenerative Diseases
- Supramolecular Chemistry and Complexes
- Antimicrobial Peptides and Activities
- Nanoparticle-Based Drug Delivery
- Biochemical and Structural Characterization
- Lipid metabolism and biosynthesis
- Photoreceptor and optogenetics research
Central South University
2019-2024
Nankai University
2016-2023
State Key Laboratory of Medicinal Chemical Biology
2017-2022
Hunan Normal University
2013-2022
Chongqing University
2019
Third Xiangya Hospital
2019
Hunan University
2009-2012
State Key Laboratory of Chemobiosensing and Chemometrics
2009-2012
Hong Kong Baptist University
2009-2012
Iowa State University
2012
Recently, the development of new fluorescent probes for ratiometric detection target objects inside living cells has received great attention. Normally, preparation, modification as well conjugation procedures these are complicated. On this basis, efforts have been paid to establish convenient method preparation dual emissive nanosensor. In work, a functional carbon dots (dCDs) was prepared by one-pot hydrothermal carbonization method. The dCDs exhibits two distinctive fluorescence emission...
Hypochlorite (ClO–) and ascorbic acid (AA) are reported to have a high correlation with oxidative stress related diseases, so it is necessary critical develop sensitive fast response sensors investigate the dynamical variation of these redox substances, especially those which can detect ClO– AA in real time two manners. However, still an unmet challenge for now. Herein, novel carbon dots (RD-CDs) respond rapidly, reversibly, dynamically by fluorescence colorimetry were synthesized. In...
Alzheimer's disease (AD) is a progressive and irreversible brain disorder. Recent studies revealed the pivotal role of β-amyloid (Aβ) in AD. However, there no conclusive indication that existing therapeutic strategies exerted any effect on mitigation Aβ-induced neurotoxicity elimination Aβ aggregates simultaneously vivo. Herein, we developed novel nanocomposite can eliminate toxic mitigate AD mice. This was designed to be small-sized particle (14 ± 4 nm) with Aβ-binding peptides (KLVFF)...
Carbon dots (CDs) are emerging as powerful tools for biosensing and bioimaging because of their intrinsic properties such abundant precursors, facile synthesis, high biocompatibility, low cost, particularly robust tunability stability. In this work, a new type CDs was prepared from m-phenylenediamine folic acid by hydrothermal method. Interestingly, the as-prepared show blue emission in non-hydrogen-bonding solution, whereas green hydrogen-bonding solution. Based on phenomenon, novel...
The complete three-dimensional orientations of single gold nanorods (AuNR) were successfully resolved by using a standard optical darkfield microscope through deciphering the field distribution pattern in slightly defocused images. resulting images depend on aspect ratio AuNR, numerical aperture objective, defocusing distance, and polarization direction incident radiation. Interpretation observed is facilitated comparing them with series simulated different parameters. experimental data...
Photodynamic therapy (PDT) has attracted great attention as an alternative tumor treatment method. Unfortunately, it suffers from some limitations like poor targeting capability and insufficient therapeutic efficiency caused by hypoxia. In this work, we introduce a novel O2-evolving PDT nanoparticle for homologous cancer cell well dual-mode imaging [i.e., magnetic resonance (MRI) fluorescence imaging]. Specifically, the nanostructure consists of MnO2 nanosheet-coated metal–organic framework...
The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although internalization process is still far from being well-understood.
Prostate-specific antigen (PSA) is an intercellular glycoprotein produced primarily by the prostate gland, which commonly chosen as initial target for early diagnosis of cancer. In this work, we demonstrate a simple yet sensitive sandwich-type single-particle enumeration (SPE) immunoassay quantitative detection PSA in flow chamber. The design based on luminescence resonance energy transfer (LRET) between upconversion nanoparticles (UCNPs) and gold (GNPs). carboxyl group-functionalized UCNPs...
Multitarget assay with single detection modality from individual stimulation mode has aroused great attention. Here, we realize multitarget the color change as signal source white light-emitting metal-organic frameworks (MOFs) under wavelength excitation for first time. The MOFs are prepared by carefully tuning ratio of Eu3+, Tb3+, and Dy3+ ions to obtain trimetal MOFs; 5-boronoisophthalic acid (5-bop) provides additional selectivity ligand. Based on antenna effect, 5-bop is excited produce...
Monitoring the ATP levels in lysosomes situ is crucial for understanding their involvement various biological processes but remains difficult due to interference of other organelles or cytoplasm. Here, we report a lysosome-specific fluorescent carbon dot (CD), which can be used detect acidic with "off-on" changes yellow fluorescence. These CDs were successfully applied real-time monitoring fluctuating concentration lysosomal induced by drug stimulation (e.g., chloroquine, etoposide, and...
Contamination of foods and feeds by aflatoxins is a universal yet serious problem all over the world. Particularly, aflatoxin B1 (AFB1) most primary form readily leads to terrible damages human health. In this work, we construct sensitive aptasensor based on single-particle detection (SPD) analyze AFB1 in peanut samples with luminescence resonance energy transfer (LRET) between aptamer-modified upconversion nanoparticles (UCNPs-aptamer) gold (GNPs). The UCNP-aptamer plays as donor, while GNP...
In a clinical assay, enzymes are essential biomarkers for human disease diagnosis. this work, spectral-resolved single-particle detection (SPD) method is introduced to quantify alkaline phosphatase (ALP) activity in serum with supraparticle (SP) based on MnO2-modified gold nanoparticle (denoted as GNP@MnO2 SP) the probe. presence of ALP, 2-phospho-l-ascorbic acid trisodium salt can be hydrolyzed into l-ascorbic acid, which serves good reduction agent trigger decomposition MnO2 shell GNP...
Dynamic changes of mitochondrial morphology play an important role in cellular metabolism. Real-time monitoring ultrastructural dynamics at nanometer-scale resolution is crucially desired for further understanding the mitochondria-based function. In this work, we introduce a fluorescent carbon dot, which can selectively target mitochondria live cells (named as MitoCD). MitoCD effectively accumulate regardless decrease or vanishing membrane potential (MMP), enabling exploration...
Artificial aquaporins are synthetic molecules that mimic the structure and function of natural (AQPs) in cell membranes. The development artificial would provide an alternative strategy for treatment AQP-related diseases. In this report, aquaporin has been constructed from amino-terminated tubular molecule, which operates a unimolecular mechanism. channel can work membranes with high water permeability selectivity rivaling those AQPs. Importantly, restore wound healing cells contain function-lost
Bimetallic nanostructures are a promising candidate for plasmon-driven photocatalysis. However, knowledge on the generation and utilization of hot carriers in bimetallic is still limited. In this work, we explored Pt position-dependent photocatalytic properties Au nanobipyramids (Au NBPs) with single-molecule fluorescence imaging. Compared all-deposited core–shell (aPt-Au NBPs), imaging simulation results show that end-deposited (ePt-Au can maintain strong electromagnetic (EM) field further...
Here we demonstrated a simple yet powerful method, planar illumination microscopy, to directly track the rotational and translational diffusion dynamics of individual anisotropic nanoparticles in solution living cells. By illuminating gold nanorods (GNRs) with two orthogonal sheets light resolving polarized scattering signal birefringent crystal, readily achieved three-dimensional angular capability for single GNRs noisy surroundings. The dispersed glycerol/water mixtures different chemical...
Plasmonic metal nanoparticles (NPs) exhibit size, shape, and composition-dependent optical properties have been studied extensively in the field of biosensing, but wide applications so far are limited by a lack both highly sensitive quantitative yet cost-effective detection schemes. We present single molecule biosensing method based on color differentiation scattered light between plasmonic NPs DNA hybridization-induced NP aggregates. With seed-mediated growth fast modification method,...
Abstract In this work, we demonstrated a convenient and green strategy for the synthesis of highly luminescent water-soluble carbon dots (Cdots) by carbonizing precursors, i.e., Bovine serum albumin (BSA) nanoparticles, in water solution. Without post surface modification, as-synthesized Cdots exhibit fluorescence quantum yield (Q.Y.) as high 34.8% display superior colloidal stability not only concentrated salt solutions (e.g. 2 M KCl) but also wide range pH solutions. According to FT-IR...
In this work, we developed a simple yet robust single particle scattering intensity measurement method for the quantification of cancer-related biomarkers. The design is based on plasmonic coupling effect between noble metal nanoparticles. First, primary and secondary antibodies were conjugated onto surface 60 nm gold nanoparticles (AuNPs, act as capture probes) 50 silver (AgNPs, signal amplification respectively. presence corresponding antigen, sandwiched immunocomplex was formed, resulting...
Rapid quantification of permanganate (MnO4–) in aqueous solution with a convenient and sensitive single-particle-detection (SPD) method is demonstrated by dark-field optical microscopy. The design based on the selective etching Ag shell glucose-protected GNPs@Ag nanoparticle MnO4–. In presence MnO4–, noticeable red-shift localized surface-plasmon resonance (LSPR, from blue to green) together tremendous decrease extinction coefficient individual nanoparticles observed. MnO4– can then be...