A5100648288- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Cassava research and cyanide
- Nematode management and characterization studies
- Hemoglobin structure and function
- Agriculture Sustainability and Environmental Impact
- Soil Carbon and Nitrogen Dynamics
- Tuberculosis Research and Epidemiology
- Agronomic Practices and Intercropping Systems
- Agriculture and Farm Safety
- Mycobacterium research and diagnosis
- Soil Mechanics and Vehicle Dynamics
- Meat and Animal Product Quality
- Vehicle Dynamics and Control Systems
- Agricultural Science and Fertilization
- Marine Sponges and Natural Products
- Fungal Biology and Applications
- Rabbits: Nutrition, Reproduction, Health
- Food Quality and Safety Studies
- Phase Change Materials Research
- Greenhouse Technology and Climate Control
- Plant Molecular Biology Research
- Genetic Syndromes and Imprinting
- Biochemical effects in animals
- Antibiotic Resistance in Bacteria
Huazhong Agricultural University
2016-2024
Gansu Agricultural University
2011-2024
China Agricultural University
2022-2024
Shanghai Jiao Tong University
2023
Northwest A&F University
2023
University of Science and Technology Liaoning
2019
Shanghai Ocean University
2019
The targeted genome editing technique, CRISPR/Cas9 system, has been widely used to modify genes of interest in a predictable and precise manner. In this study, we describe the CRISPR/Cas9-mediated efficient representative SNF (symbiotic nitrogen fixation) related model legume Lotus japonicus via Agrobacterium-mediated stable or hairy root transformation. We first predicted nine endogenous U6 then demonstrated efficacy LjU6-1 gene promoter driving expression single guide RNAs (sgRNAs) by...
Cowpea (Vigna unguiculata) is widely cultivated across the world. Due to its symbiotic nitrogen fixation capability and many agronomically important traits, such as tolerance low rainfall fertilization requirements, well high nutrition health benefits, cowpea an legume crop, especially in semi-arid countries. However, research Vigna unguiculata dramatically hampered by lack of mutant resources efficient tools for gene inactivation vivo. In this study, we used clustered regularly interspaced...
Legume nodules contain high concentrations of leghemoglobins (Lbs) encoded by several genes. The reason for this multiplicity is unknown. CRISPR/Cas9 technology was used to generate stable mutants the three Lbs Lotus japonicus. phenotypes were characterized at physiological, biochemical and molecular levels. Nodules triple examined electron microscopy subjected RNA-sequencing (RNA-seq) analysis. Complementation studies revealed that function synergistically maintain optimal N2 fixation....
Summary Legumes establish symbioses with rhizobia by forming nitrogen‐fixing nodules. Nitrate is a major environmental factor that affects symbiotic functioning. However, the molecular mechanism of nitrate‐induced nodule senescence poorly understood. Comparative transcriptomic analysis reveals an NAC‐type transcription in Lotus japonicus , LjNAC094, acts as positive regulator senescence. Stable overexpression and mutant lines NAC094 were constructed used for phenotypic characterization....
Excessive application of nitrogen fertilizers has inevitably resulted in environmental problems. The symbiotic fixation (SNF) that occurs the root nodules leguminous plants provides a sustainable source reduced agricultural ecosystems. More than 200 genes have been reported to regulate SNF, including rhizobial infection, nodule organogenesis and senescence (Roy et al., 2020). Mature consist mainly two cell types: infected cells (IC) contain nitrogen-fixing bacteroids uninfected (UC) mediate...
Introduction Utilizing roughage resources is an effective approach to alleviate the shortage of corn-soybean feed and reducing costs in swine industry. Hezuo pig one group plateau type local Tibetan with strong tolerance crude feeding. Nevertheless, current research on pigs microbiological mechanisms behind it still minimally.This study explored impact various ratios whole-plant silage (WPS) maize pH, cellulase activity, short-chain fatty acids (SCFAs), intestinal microbiota pigs. Methods...
Carbonic anhydrase (CA) plays a vital role in photosynthetic tissues of higher plants, whereas its non-photosynthetic the symbiotic root nodule was rarely characterized. In this study, 13 CA genes were identified model legume Lotus japonicus by comparison with Arabidopsis genes. Using qPCR and promoter-reporter fusion methods, three previously nodule-enhanced (LjαCA2, LjαCA6, LjβCA1) have been further characterized, which exhibit different spatiotemporal expression patterns during...
The Hezuo pig, an important native Tibetan breed in China, exhibits differences adult body weight, with females typically heavier than males. underlying mechanisms for this disparity remain unclear. DNA methylation changes are known to influence animal growth and development regulate pig by altering gene expression related these processes, thus differentially affecting weight between genders. This study conducted analysis profiling using pituitary tissues from male female pigs at 3 8 months...
Whole-plant corn silage (WPCS) is an important roughage source for livestock, and have critical influences on rumen or intestinal microbiota, thus affecting the growth performance feed efficiency. Our previous studies showed that adding WPCS to diet of Hezuo pigs could promote fiber digestibility. While aim this study understand effect dietary colonic microflora in pigs, which essential improving exploitation pigs.
Legume nodules produce large quantities of heme required for the synthesis leghemoglobin (Lb) and other hemoproteins. Despite crucial function Lb in nitrogen fixation toxicity free heme, mechanisms homeostasis remain elusive. Biochemical, cellular, genetic approaches were used to study role oxygenases (HOs) degradation model legume Lotus japonicus. Heme biliverdin quantified localized, HOs characterized, knockout LORE1 CRISPR/Cas9 mutants LjHO1 generated phenotyped. We show that LjHO1, but...
Abstract Legume nodules express multiple leghemoglobins (Lbs) and non-symbiotic hemoglobins (Glbs), but how they are regulated is unclear. Here, we study the regulation of all Lbs Glbs Lotus japonicus in different physiologically relevant conditions mutant backgrounds. We quantified hemoglobin expression, localized reactive oxygen species (ROS) nitric oxide (NO) nodules, deployed mutants deficient transcription factors NLP4 (associated with nitrate sensitivity) NAC094 senescence). Expression...
Legumes express two major types of hemoglobins, namely symbiotic (leghemoglobins) and non-symbiotic (phytoglobins), with the latter being categorized into three classes according to phylogeny biochemistry. Using knockout mutants, we show that all phytoglobin are required for optimal vegetative reproductive development Lotus japonicus. The mutants class 1 phytoglobins showed different phenotypes: Ljglb1-1 plants were smaller had relatively more pods, whereas Ljglb1-2 no distinctive phenotype...
The mutualistic beneficial relationship between legume plants and rhizobia enables the growth of in nitrogen-limiting conditions. Rhizobia infect legumes through root hairs trigger nodule organogenesis cortex. plant hormone cytokinin plays a pivotal role regulating both rhizobial infection initiation development. However, mechanism used by output module to control symbiosis remains poorly documented. In this study, we identified signaling component encoded Type-B RESPONSE REGULATOR (RRB)...
Objective: Because infections caused by nontuberculous mycobacteria (NTM) are rapidly increasing globally, a need exists for developing novel antibiotics and discovering the mechanism of resistance. This research was aimed at understanding bedaquiline resistance in model NTM species Mycobacterium marinum ( M. ). Methods: The M strain subjected to mutant selection with different concentrations BDQ. After three rounds evolution, 58 BDQ-resistant mutants were isolated WGS. results confirmed...
Abstract α-Damascone is widely used in perfumes. However, the manufacture of α-damascone remains challenging owing to limitations current production processes. Herein, was successfully synthesized from α-ionone using a new route involving only four steps, namely oximization, epoxidation, dehydration, and reduction. The total yield 54.9% with final chemical purity 97% (by GC). Only water, cyclohexane, ethanol were reactions except purification step, all solvents could be recycled. structures...
Crop straw is an important biomass resource in green, sustainable and intensive agricultural production. It can affect the microbial community structure farmland ecosystem by changing physical, chemical, biological properties of soil. However, it remains unclear how amount returning influence soil nutrients, crop yield, phospholipid (PLFAs) bacterial functional. For this, a long-term maize cropping field experiment was conducted semi-arid area China under five treatments: no (T0); with 3000...