A5079588717- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Tuberculosis Research and Epidemiology
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- Infectious Encephalopathies and Encephalitis
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Virology and Viral Diseases
- Infectious Diseases and Tuberculosis
- Herpesvirus Infections and Treatments
- Immunodeficiency and Autoimmune Disorders
- Respiratory viral infections research
- Immune responses and vaccinations
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Diagnosis and treatment of tuberculosis
- Advanced Proteomics Techniques and Applications
- Leprosy Research and Treatment
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Streptococcal Infections and Treatments
- Advanced Biosensing Techniques and Applications
Center for Infection and Immunity of Lille
2014-2024
Inserm
2014-2024
Institut Pasteur de Lille
2012-2022
Centre National de la Recherche Scientifique
2012-2022
Université de Lille
2012-2022
Centre Hospitalier Universitaire de Lille
2016-2022
Centre National pour la Recherche Scientifique et Technique (CNRST)
2017
Université Lille Nord de France
2010-2014
École Nationale Supérieure de Chimie de Lille
2011
Institut Pasteur
2003-2004
Roland Brosch and colleagues report whole-genome sequencing of tubercle bacilli with smooth colony morphology, also known as Mycobacterium canetti and/or prototuberculosis, obtained from humans tuberculosis geographically diverse regions. Their comparative genomic analysis other M. complex members identifies extensive recombination provides insights into the evolution mycobacteria. The authors that morphology show reduced persistence virulence compared to strains in infection experiments...
Pertussis is still among the principal causes of death worldwide, and its incidence increasing even in countries with high vaccine coverage. Although all age groups are susceptible, it most severe infants too young to be protected by currently available vaccines. To induce strong protective immunity neonates, we have developed BPZE1, a live attenuated Bordetella pertussis strain given as single-dose nasal early life. BPZE1 was genetic inactivation or removal three major toxins. In mice,...
Rationale: Tuberculosis (TB) remains a leading cause of death, and the role T-cell responses to control Mycobacterium tuberculosis infections is well recognized. Patients with latent TB infection develop strong IFN-γ protective antigen heparin-binding hemagglutinin (HBHA), whereas patients active do not.Objectives: We investigated mechanism this difference evaluated possible involvement regulatory T (Treg) cells and/or cytokines in low HBHA TB.Methods: The impact anti–transforming growth...
Background Acellular pertussis vaccines do not control pertussis. A new approach to offer protection infants is necessary. BPZE1, a genetically modified Bordetella strain, was developed as live attenuated nasal vaccine by eliminating or detoxifying 3 toxins. Methods We performed double-blind, placebo-controlled, dose-escalating study of BPZE1 given intranasally for the first time human volunteers, trial bacterial specifically designed respiratory tract. 12 subjects per dose group received...
Abstract Neonatal immaturity of the immune system is currently believed to generally limit induction responses vaccine Ags and skew them toward type 2 responses. We demonstrated here that Bordetella pertussis infection in very young infants (median, mo old) as well first administration whole-cell induces B. Ag-specific IFN-γ secretion by PBMC these infants. was secreted both CD4+ CD8+ T lymphocytes, levels Ag-induced did not correlate with age Appearance specific Th-1 cell-mediated immunity...
BackgroundThe detection of latent tuberculosis infection (LTBI) is a major component (TB) control strategies. In addition to the skin test (TST), novel blood tests, based on in vitro release IFN-γ response Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 (IGRAs), are used for TB diagnosis. However, neither IGRAs nor TST can separate acute from LTBI, there concern that responses may decline with time after infection. We have therefore evaluated potential antigen heparin-binding...
Evidence suggests that the resurgence of pertussis in many industrialized countries may result from failure current vaccines to prevent nasopharyngeal colonization by Bordetella pertussis, principal causative agent whooping cough. Here, we used a baboon model test protective potential novel, live attenuated vaccine candidate BPZE1. A single intranasal/intratracheal inoculation juvenile baboons with BPZE1 resulted transient and induction immunoglobulin G all antigens tested, while causing no...
Pathogens have evolved a range of mechanisms to counteract host defenses, notably survive harsh acidic conditions in phagosomes. In the case Mycobacterium tuberculosis, it has been shown that regulation phagosome acidification could be achieved by interfering with retention V-ATPase complexes at vacuole. Here, we present evidence M. tuberculosis resorts yet another strategy control phagosomal acidification, suppressor cytokine signaling (SOCS) protein functions. More precisely, show...
Abstract Mycobacterium tuberculosis is a successful intracellular pathogen. Numerous host innate immune responses signaling pathways are induced upon mycobacterium invasion, however their impact on M. replication not fully understood. Here we reinvestigate the role of STAT3 specifically inside human macrophages shortly after uptake. We first show that activation mediated by IL-10 and occurs in infected cells as well bystander non-colonized cells. results inhibition IL-6, TNF-α, IFN-γ MIP-1β....
Abstract Pertussis has made a spectacular rebound in countries that have switched from whole-cell (wPV) to acellular pertussis vaccines (aPV). Here, we show that, unlike wPV, aPV, while protective against lung colonization by Bordetella (Bp), did not protect BALB/c mice nasal colonization, but instead substantially prolonged carriage. aPV prevented the natural induction of interleukin-17 (IL-17)-producing and interferon-γ (IFN-γ)-producing CD103 + CD44 CD69 CD4 -resident memory T (T RM )...
Mycobacterium tuberculosis and bovis bacillus Calmette–Guérin produce a heparin-binding hemagglutinin adhesin (HBHA) required for extrapulmonary dissemination laminin-binding protein (LBP) involved in cytoadherence through laminin recognition. These adhesins bear posttranslational modifications that are not present when the proteins produced recombinant (r) form Escherichia coli . Mass spectrometry analysis of HBHA revealed borne by C-terminal moiety, which comprises domain made repeated...
Multi-drug-resistant tuberculosis (TB) is a major public health problem, concerning about half million cases each year. Patients hardly adhere to the current strict treatment consisting of more than 10 000 tablets over 2-year period. There clear need for efficient and better formulated medications. We have previously shown that nanoparticles made cross-linked poly-β-cyclodextrins (pβCD) are vehicles pulmonary delivery powerful combinations anti-TB drugs. Here, we report in addition being...
ABSTRACT The heparin-binding hemagglutinin (HBHA) of Mycobacterium tuberculosis is a surface-expressed adhesin that can affect binding to host cells via unique, methylated, carboxyl-terminal, lysine-, alanine-, and proline-rich repeat region. It has been implicated in extrapulmonary dissemination M. from the lung following initial infection host. To assess vaccine potential this protein, purified preparations HBHA were emulsified dimethyldioctadecylammonium bromide-monophosphoryl lipid A...
Abstract New vaccines against pertussis are needed to evoke full protection and long-lasting immunological memory starting from the first administration in neonates—the major target of life-threatening infection. A novel live attenuated Bordetella vaccine strain, BPZE1, has been developed by eliminating or detoxifying three important B. virulence factors: toxin, dermonecrotic tracheal cytotoxin. We used a human preclinical ex vivo model based on monocyte-derived dendritic cells (MDDCs)...
Despite the availability of efficacious vaccines, incidence whooping cough is still high in many countries and even increasing with vaccine coverage. Most severe life-threatening pertussis cases occur infants who are too young to be sufficiently protected by current regimens. As a potential solution this problem, we have developed an attenuated live Bordetella strain, named BPZE1. Here, show that after single administration, BPZE1 induces dose-dependent protection against challenge virulent...
BackgroundMost individuals infected with Mycobacterium tuberculosis do not develop (TB) and can be regarded as being protected by an appropriate immune response to the infection. The characterization of responses latent TB may thus helpful in definition correlates protection development new vaccine strategies. highly protective antigen heparin-binding hemagglutinin (HBHA) induces strong interferon (IFN)–γ during latent, but active, TB. Because recently recognized importance CD8+ T...