A5084227533- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
- Infective Endocarditis Diagnosis and Management
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Antimicrobial Resistance in Staphylococcus
- Vector-borne infectious diseases
- Immune Response and Inflammation
- Actinomycetales infections and treatment
- Gut microbiota and health
- Respiratory viral infections research
- Diphtheria, Corynebacterium, and Tetanus
- Vector-Borne Animal Diseases
- Peripheral Neuropathies and Disorders
- Biochemical and Structural Characterization
- Legionella and Acanthamoeba research
- Infectious Encephalopathies and Encephalitis
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Herpesvirus Infections and Treatments
- Streptococcal Infections and Treatments
- Vibrio bacteria research studies
Center for Infection and Immunity of Lille
2011-2025
Centre National de la Recherche Scientifique
2011-2025
Centre Hospitalier Universitaire de Lille
2017-2025
Université de Lille
2011-2025
Inserm
2004-2025
Institut Pasteur de Lille
2003-2024
Paris-Est Sup
2021
Université Paris-Est Créteil
2021
Assistance Publique – Hôpitaux de Paris
2021
Hôpitaux Universitaires Henri-Mondor
2019
The identification of genes important in the pathogenesis Lyme disease Borrelia has been hampered by exceedingly low transformation rates low-passage, infectious organisms. Using infectious, moderately transformable B. burgdorferi derivative 5A18NP1 and signature-tagged versions Himar1 transposon vector pGKT, we have constructed a defined library for efficient genome-wide investigation required wild-type pathogenesis, vitro growth, physiology, morphology, plasmid replication. To facilitate...
Lyme disease Borrelia can infect humans and animals for months to years, despite the presence of an active host immune response. The vls antigenic variation system, which expresses surface-exposed lipoprotein VlsE, plays a major role in B. burgdorferi evasion. Gene conversion between silent cassettes vlsE expression site occurs at high frequency during mammalian infection, resulting sequence VlsE product. In this study, we examined B31 mouse infection by analyzing 1,399 clones isolated from...
Abstract Pertussis has made a spectacular rebound in countries that have switched from whole-cell (wPV) to acellular pertussis vaccines (aPV). Here, we show that, unlike wPV, aPV, while protective against lung colonization by Bordetella (Bp), did not protect BALB/c mice nasal colonization, but instead substantially prolonged carriage. aPV prevented the natural induction of interleukin-17 (IL-17)-producing and interferon-γ (IFN-γ)-producing CD103 + CD44 CD69 CD4 -resident memory T (T RM )...
Pathogen attachment is a crucial early step in mucosal infections. This mediated by important virulence factors called adhesins. To exert these functions, adhesins are typically surface-exposed, although, surprisingly, some also released into the extracellular milieu, relevance of which has previously not been studied. address role adhesin release pathogenesis, we used Bordetella pertussis as model, since its major adhesin, filamentous hemagglutinin (FHA), partitions between bacterial...
Insertion sequences (IS) represent mobile genetic elements that have been shown to be associated with bacterial evolution and adaptation due their effects on genome plasticity. In Bordetella pertussis, the causative agent of whooping cough, numerous IS induce genomic rearrangements contribute diversity global B. pertussis population. Previously, we majority IS-specific endogenous promoters synthesis alternative transcripts thereby affect transcriptional landscape pertussis. Here, describe...
ABSTRACT An in silico scan of the partially completed genome sequence Bordetella pertussis and analyses transcriptional fusions generated with a new integrational vector were used to identify potential virulence genes. The genes encoding putative siderophore receptor, adhesins, an autotransporter protein appeared be regulated manner similar by global regulator BvgAS. In contrast, gene intimin-like repressed under conditions virulence.
Abstract The whooping cough agent Bordetella pertussis regulates the production of its virulence factors by BvgA/S system. Phosphorylated BvgA activates virulence-activated genes ( vag s) and represses expression virulence-repressed vrg via activation bvgR gene. In modulating conditions, with MgSO 4 , system is inactive, s are expressed. Here, we show that almost all depends on RisA, another transcriptional regulator. We also some surprisingly no longer modulated in risA − background. RisA...
Bordetella pertussis is the causative agent of whooping cough, a respiratory disease still considered as major public health threat and for which recent re-emergence has been observed. Constant reshuffling genome organization was observed during evolution. These rearrangements are essentially mediated by Insertion Sequences (IS), mobile genetic elements present in more than 230 copies genome, supposed to be one driving forces enabling pathogen escape from vaccine-induced immunity.Here we use...
Summary Many extracytoplasmic proteins undergo proteolytic processing during secretion, which is essential to their maturation. These post‐translational modifications are carried out by specific enzymes whose subcellular localization important for function. We have described a maturation subtilisin in Gram‐negative Bordetella pertussis , the autotransporter SphB1. SphB1 catalyses of precursor adhesin filamentous haemagglutinin (FHA) at bacterial surface, addition its own precursor. Here, we...
Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from 11 VirB subunits VirD4. Two subunits, VirB9 VirB7, form an important stabilizing complex outer membrane. We describe here NMR structure a between C-terminal domain homolog TraO (TraO(CT)), bound to VirB7-like TraN plasmid pKM101. TraO(CT) forms beta-sandwich around which winds. Structure-based...
Context: Today, infective endocarditis (IE) caused by Enterococcus faecalis represents 10% of all IE and is marked its difficult management the frequency relapses. Although precise reasons for that remain to be elucidated, evolution culprit strain under selective pressure through microdiversification could be, at least in part, involved. Material methods: To further study situ genetic microdiversity possible phenotypic manifestations E. IE, we sequenced compared multiple isolates from...
The regulation of
Bordetella pertussis regulates the production of its virulence factors by two-component system BvgAS. In phase, BvgS phosphorylates BvgA, which then activates transcription virulence-activated genes (vags). avirulence such as during growth in presence MgSO4, BvgA is not phosphorylated and vags are expressed. Instead, a set virulence-repressed (vrgs) Here, we performed transcriptome sequencing (RNAseq) analyses on B. cultivated with or without MgSO4 BvgA-deficient Tohama I derivative. We...
Recent whooping cough (pertussis) outbreaks in many countries highlight the crucial need for a better understanding of pathogenesis Bordetella pertussis infection respiratory tract. The baboon is recently described preclinical model study B. and may be ideal evaluation new vaccines. However, pathophysiological aspects, including bacterial localization interactions, have yet to this model. Here, we used with fluorescent GFP-expressing strain, derived from European clinical isolate B1917....
Current pertussis vaccines protect against disease, but not colonization by and transmission of Bordetella pertussis, whereas natural infection protects both. The live attenuated vaccine BPZE1 was developed to mimic immunogenicity without causing in preclinical models protected disease B. after a single nasal administration. Phase 1 clinical studies showed that is safe immunogenic humans when administered as liquid formulation, stored at ≤-70 °C. Although stable for two years °C, lyophilized...