A5101988821- Cardiac electrophysiology and arrhythmias
- Cancer therapeutics and mechanisms
- Neuroscience and Neural Engineering
- Drug Transport and Resistance Mechanisms
- Colorectal Cancer Treatments and Studies
- Ion channel regulation and function
- Genomics, phytochemicals, and oxidative stress
- Cancer Treatment and Pharmacology
- Cellular transport and secretion
- Cardiac Arrhythmias and Treatments
- Lung Cancer Research Studies
- Bioactive Compounds and Antitumor Agents
- Gastric Cancer Management and Outcomes
- Exercise and Physiological Responses
- Atrial Fibrillation Management and Outcomes
- Chemical Reactions and Isotopes
- Cancer Mechanisms and Therapy
- Lung Cancer Treatments and Mutations
- Pain Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Calcium signaling and nucleotide metabolism
- Renal and Vascular Pathologies
Saitama Medical University
2006-2025
Social Insurance Saitama Chuo Hospital
2025
Teijin (Japan)
2010-2017
Japan Medical Association
2017
Tokyo University of Science
2014-2015
Yokohama City University Medical Center
2015
Yokohama City University
2015
Kanagawa Cancer Center
2013
Kanagawa Prefectural Hospital Organization
2013
New York Blood Center
2011
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are anticipated to be a useful tool for conducting proarrhythmia risk assessments of drug candidates. However, torsadogenic prediction paradigm using hiPSC-CMs has not yet been fully established.Extracellular field potentials (FPs) were recorded from the multi-electrode array (MEA) system. The effects on FPs evaluated with 60 drugs, including 57 various clinical risks. Actual concentrations in medium measured equilibrium...
The aims of this study were to (1) characterize basic electrophysiological elements human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) that correspond clinical properties such as QT-RR relationship, (2) determine the applicability QT correction and analysis methods, (3) if how these in-vitro parameters could be used in risk assessment for adverse drug-induced effects Torsades de pointes (TdP). Field potential recordings obtained from commercially available hiPSC-CMs using...
The acetylcholine-activated K(+) current (I(K,ACh)) is a novel candidate for atrial-specific antiarrhythmic therapy. present study investigates the involvement of I(K,ACh) in atrial fibrillation (AF) using NTC-801, potent and selective blocker.The effects substituted 4-(aralkylamino)-2,2-dimethyl-3,4-dihydro-2H-benzopyran-3-ol, on other cardiac ionic currents (I(Na), I(CaL), I(to), I(Kur), I(Kr), I(Ks), I(Kl), I(KATP), I(f)) ventricular action potentials were examined vitro. NTC-801 potently...
S‐1 is an oral anticancer agent composed of tegafur (FT), 5‐chloro‐2,4‐dihydroxypyridine (CDHP), and potassium oxonate. CDHP added to prevent degradation 5‐fluorouracil (5‐FU) by inhibiting dihydropyrimidine dehydrogenase. CYP2A6 involved in the biotransformation FT 5‐FU. Thus, we prospectively analyzed effects genotype, plasma level CDHP, patient characteristics on pharmacokinetic (PK) variability Fifty‐four Japanese patients with metastatic or recurrent cancers who received were enrolled....
ATP-binding cassette, sub-family C, number 2 (ABCC2) is involved in the biliary excretion of irinotecan and its metabolites, SN-38 glucuronide. Effects ABCC2 genotype on pharmacokinetics (PK) metabolites were examined Japanese patients with metastatic colorectal cancer receiving plus infusional 5-fluorouracil/leucovorin (FOLFIRI). genotypes (−1549G>A, −1023G>A, −1019A>G, −24C>T, 1249G>A 3972C>T) haplotypes analyzed for 67 cancer. PK was also a subset 31 FOLFIRI. Relationship between or...
Mechanical allodynia, such as static and dynamic is a prominent feature of neuropathic pain syndromes. The aim this study to characterize primary sensory neurons mediating the mechanical allodynia in rat chronic constriction injury (CCI) model with combination pharmacological histological investigations. N-(4-Tertiarybutylphenyl)-4-(3-chloropyridin-2-yl) tetrahydropyrazine-1(2H)-carbox-amide (BCTC), selective competitive antagonist vanilloid receptor 1 (TRPV1), resiniferatoxin, which causes...
Irinotecan (CPT-11) is a camptothecin analog with low (about 10-20%) and variable oral bioavailability in animal models. Here, Caco-2 cells were used to evaluate the transepithelial transport of CPT-11 its metabolites. demonstrated significant expression P-glycoprotein (P-gp), multidrug resistance-associated protein canalicular multispecific organic anion transporter. Both lactone carboxylate forms SN-38 actively transported across cell monolayers, mainly by apical-localized P-gp pump....
The present study examines the effects of NTC-801, a highly selective acetylcholine (ACh) receptor-activated potassium (KACh) channel blocker, on atrial fibrillation (AF) in canine model with electrical remodeling. An experimental substrate for AF was created dogs via left (LA) tachypacing (400 bpm, 3-5 weeks). dofetilide, and flecainide were intravenously infused 15 minutes, inducibility, effective refractory period (ERP), conduction velocity examined. effect NTC-801 termination also...
We attempted to determine a target of chemotherapy specific glioblastoma cells ensure favorable response anticancer drugs, through comparison in biologic nature related drug resistance with other types cancer cells. Using 13 human cell lines including 3 lines, gene expression analysis and biochemical quantitative assay were performed for total 12 properties, which have been linked action. Although most genes resistance, such as MDR1, MRP, MGMT GSTpi, overexpressed T98G, U-373MG, U-251MG...
The precise mechanisms of resistance to camptothecin (CPT)-derived DNA topoisomerase (topo I) inhibitors and the determinants remain unclear. We found that a repair protein, O(6)-methylguanine-DNA methyltransferase (MGMT), participated in irinotecan hydrochloride (CPT-11), its active metabolite SN-38, novel CPT derivative, DX-8951f. In 17 human cancer cell lines, MGMT gene expression level closely correlated with sensitivity derivatives, inhibition activity by nontoxic 5 microM...
The dynamic assembly and disassembly of actin filaments is essential for the formation transport vesicles during endocytosis. In yeast, two types structures, namely cortical patches cytoplasmic cables, play a direct role in endocytosis, but how their interaction regulated remains unclear. Here we show that Srv2/CAP, an evolutionally conserved regulator, required efficient endocytosis due to its aid initial vesicle invagination cables these move along. Deletion SRV2 gene results appearance...
β-tubulin (β-TUB), Bcl-XL, and additionally glutathione S-transferase π (GSTπ) were found to participate in sensitivity docetaxel (TXT) 7 human gastrointestinal cancer cell lines. The gene expression level of β-TUB, GSTπ was closely correlated with the IC50 for TXT. β-TUB amount related TXT resistance, GST activity 30-min treatment setting. Bcl-XL transfection increased resistance COLO201 cells, whereas inhibition by ethacrynic acid enhanced cytotoxicity. Continuous expression, but mRNA...
Gefitinib (Iressa) is an anticancer drug that selectively inhibits tyrosine kinases of epidermal growth factor receptor. might affect CYP3A4-mediated metabolism, since the a substrate human CYP3A. In this study, we evaluated effects gefitinib on metabolism catalyzed by CYP3A4. The formation NPC (7-ethyl-10-(4-amino-1-piperidino)carbonyloxycamptothecin) and APC (7-ethyl-10-[4-<i>N</i>-(5-aminopentanoic acid)-1-piperidino]carbonyloxycamptothecin) from irinotecan were examined with use liver...
ObjectiveThis phase I/II study determined the recommended dose of FOLFIRI (irinotecan, infusional 5-fluorouracil and leucovorin) for Japanese patients with advanced colorectal cancer, evaluated safety at in without UDP-glucuronosyltransferase 1A1*28 allele which caused reduced enzyme expression.
"Musculoskeletal pain may be associated with imatinib withdrawal syndrome in chronic myeloid leukemia patients." Leukemia & Lymphoma, 57(2), pp. 496–497
The author presents a statistical analysis of foreign bodies in the air and food passages based on data obtained from 37 medical school hospitals throughout Japan.Of 7371 total number cases 1571 (20.3%) were respiratory tract 5800 (79.7%) passage; latter constitutes 3.6 times former.Sex: Of number, 4365 (59.3%) males 3006 (40.7%) females: ratio between two sexes is 3 to 2. In passage accounted 891 females 650, 3444 2356 respectively.Age: my experience youngest patient was one month old...
A network composed of activation and inactivation pathways to regulate mitomycin C (MMC) action is suggested exist in human cancer cells. COLO201 colon cells were stably transfected with NQO1 cDNA that encodes NAD(P)H:quinone oxidoreductase (DT-diaphorase, DTD), a clonal cell line about 57-fold elevated DTD activity was obtained. Northern analysis revealed expression the NADPH:cytochrome P450 reductase (P450 reductase) gene decreased transfectant, COLO201/NQO1, associated increase...