A5078437281- Neural dynamics and brain function
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Context-Aware Activity Recognition Systems
- Analog and Mixed-Signal Circuit Design
- Sleep and Wakefulness Research
- Genomics and Phylogenetic Studies
- Imbalanced Data Classification Techniques
- COVID-19 Clinical Research Studies
- Pneumonia and Respiratory Infections
- Poisoning and overdose treatments
- Photoreceptor and optogenetics research
- Viral Infectious Diseases and Gene Expression in Insects
- Cystic Fibrosis Research Advances
- Receptor Mechanisms and Signaling
- Data Quality and Management
- Microbial Metabolic Engineering and Bioproduction
- Gene Regulatory Network Analysis
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Green IT and Sustainability
- Immune Cell Function and Interaction
Northwestern University
2013-2024
Shirley Ryan AbilityLab
2014-2018
Simultaneously measuring the activities of all neurons in a mammalian brain at millisecond resolution is challenge beyond limits existing techniques neuroscience. Entirely new approaches may be required, motivating an analysis fundamental physical constraints on problem. We outline principles governing activity mapping using optical, electrical,magnetic resonance, and molecular modalities neural recording. Focusing mouse brain, we analyze scalability each method, concentrating limitations...
Sleep is a critical aspect of people's well-being and as such assessing sleep an important indicator person's health. Traditional methods assessment are either time- resource-intensive or suffer from self-reporting biases. Recently, researchers have started to use mobile phones passively assess in individuals' daily lives. However, this work remains its early stages, having only examined relatively small homogeneous populations carefully controlled contexts. Thus, it open question how well...
Abstract Sweat parameters such as volume and chloride concentration may offer invaluable clinical insights for people with CF (PwCF). Pilocarpine-induced sweat collection chloridometry measurement is the gold-standard chloride, but this technique cumbersome not suitable remote settings. We have previously reported utility of a skin-interfaced microfluidic device (CF Patch) in conjunction smartphone image processing platform that enables real-time sweating rates sodium loss laboratory Here we...
DNA polymerase fidelity is affected by both intrinsic properties and environmental conditions. Current strategies for measuring error rate in vitro are constrained low subtype sensitivity, poor scalability, lack of flexibility types sequence contexts that can be tested. We have developed the Magnification via Nucleotide Imbalance Fidelity (MagNIFi) assay, a scalable next-generation sequencing assay uses biased deoxynucleotide pool to quantitatively shift rates into range where errors...
Naturally evolved metabolite-responsive biosensors enable applications in metabolic engineering, ranging from screening large genetic libraries to dynamically regulating biosynthetic pathways. However, there are many metabolites for which a natural biosensor does not exist. To address this need, we developed general method converting metabolite-binding proteins into transcription factors-Biosensor Engineering by Random Domain Insertion (BERDI). This approach takes advantage of an vitro...
To record from a given neuron, recording technology must be able to separate the activity of that neuron its neighbors. Here, we develop Fisher information based framework determine conditions under which this is feasible for technology. This combines measurable point spread functions with noise distributions produce theoretical bounds on precision can localize neural activities. If there suffi□cient uniquely activities, then will, an theoretic perspective, these neurons. We (1) describe...
One of the greatest challenges an organization faces is determining when employee permitted to utilize a certain resource in system. This "insider threat" can be addressed through two strategies: i) prospective methods, such as access control, that make decision at time request, and ii) retrospective post hoc auditing, light knowledge gathered afterwards. While it recognized each strategy has distinct set benefits drawbacks, there been little investigation into how provide system...
Abstract A central issue in neural recording is that of distinguishing the activities many neurons. Here, we develop a framework, based on Fisher information, to quantify how separable neuron’s activity from nearby We (1) apply this framework model information flow and spatial distinguishability for several electrical optical methods, (2) provide analytic expressions content, (3) demonstrate potential applications approach. This method generalizes devices resolve objects space thus may be...
Abstract Objectives Critical illness reduces β-lactam pharmacokinetic/pharmacodynamic (PK/PD) attainment. We sought to quantify PK/PD attainment in patients with hospital-acquired pneumonia. Methods Meropenem plasma PK data (n = 70 patients) were modelled, rates calculated for empirical and definitive targets, between-patient variability was quantified [as a coefficient of variation (CV%)]. Results Attainment 100% T>4×MIC variable both (CV% 92) directed 33%) treatment. Conclusions...
Abstract Advancements in synthetic biology have provided new opportunities biosensing with applications ranging from genetic programming to diagnostics. Next generation biosensors aim expand the number of accessible environments for measurement, increase measurable phenomena, and improve quality measurement. To this end, an emerging area field has been integration DNA as information storage medium within biosensor outputs, leveraging nucleic acids record state over time. However, slow signal...
Advancements in synthetic biology have provided new opportunities biosensing, with applications ranging from genetic programming to diagnostics. Next generation biosensors aim expand the number of accessible environments for measurements, increase measurable phenomena, and improve quality measurement. To this end, an emerging area field has been integration DNA as information storage medium within biosensor outputs, leveraging nucleic acids record state over time. However, slow signal...
Using a DNA polymerase to record intracellular calcium levels has been proposed as novel neural recording technique, promising massive-scale, single-cell resolution monitoring of large portions the brain. This technique relies on local storage activity in strands DNA, followed by offline analysis that DNA. In simple implementations this scheme, time when each nucleotide was written cannot be determined directly post-hoc sequencing; timing data must estimated instead. Here, we use Dynamic...