A5105769945- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Ion Channels and Receptors
- Aluminum toxicity and tolerance in plants and animals
- Cardiomyopathy and Myosin Studies
- Immune Response and Inflammation
- DNA Repair Mechanisms
- Neonatal and fetal brain pathology
- Cardiac electrophysiology and arrhythmias
- Immunotherapy and Immune Responses
- Parasitic Infections and Diagnostics
- Epilepsy research and treatment
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Advanced Glycation End Products research
- Virus-based gene therapy research
- Magnesium in Health and Disease
- Cardiovascular Function and Risk Factors
- Collagen: Extraction and Characterization
University of Auckland
2008-2023
Yellow River Conservancy Technical Institute
2016
Tsinghua University
2001-2008
Abstract Hyaluronan is a linear glycosaminoglycan that forms the backbone of perineuronal nets around neurons in cerebral cortex. However, it remains controversial whether are capable independent hyaluronan synthesis. Herein, we examined expression and synthases (HASs) throughout cortical neuron development vitro . Enriched cultures were established from E16 rats. Neurons collected at days (DIV) 0 (4 h), 1, 3, 7, 14, 21 for qPCR or immunocytochemistry. In relative absence glia, exhibited...
Collagen VI has recently been strongly linked to poor outcomes in heart failure with preserved ejection fraction through increased endotrophin, a collagen VI-derived signalling molecule fibrotic remodelling cardiovascular disease. The mutation of can result Ullrich congenital muscular dystrophy and Bethlem myopathy, pointing critical function muscle physiology. However, the role is poorly understood. In human reduced fraction, within remodelled T-tubules, suggesting possible tubular...
Abstract Background Survivin is a member of the inhibitor-of-apoptosis (IAP) family which widely expressed by many different cancers. Overexpression survivin associated with drug resistance in cancer cells, and reduced patient survival after chemotherapy radiotherapy. Agents that antagonize function hold promise for treating forms cancer. The purpose this study was to investigate whether cell-permeable dominant-negative protein would demonstrate bioactivity against prostate cervical cells...
Objective Although the spectrum of white matter injury (WMI) in preterm infants is shifting from cystic necrotic lesions to milder forms, factors that contribute this changing are unclear. We hypothesized recurrent hypoxia-ischemia (rHI) will exacerbate WMI defined by markers inflammation and molecules related extracellular matrix (hyaluronan (HA) PH20 hyaluronidase) regulate maturation oligodendrocyte (OL) lineage after WMI. Methods employed a fetal sheep model utero moderate hypoxemia...
Clara cell protein (CC16) is a well-known anti-inflammatory secreted by the epithelial cells of airways. It involved in development airway inflammatory diseases such as chronic obstructive pulmonary disease and asthma. Previous studies suggest that CC16 gene transfer suppresses expression interleukin (IL)-8 bronchial cells. However, its role function these during inflammation not well understood. In this study, we evaluated effect on matrix metalloproteinase (MMP)-9 lipopolysaccharide...
Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite that infects a variety of mammals, including humans. An effective vaccine for this therefore needed. In study, RH strain T. rhoptry protein 17 was expressed in bacteria as fusion with glutathione S-transferase (GST) and the recombinant proteins (rTgROP17) were purified via GST-affinity chromatography. BALB/c mice nasally immunised rTgROP17, induction immune responses protection against chronic lethal infections...
Toxoplasma gondii is an obligate intracellular apicomplexan parasite that affects humans and various vertebrate livestock causes serious economic losses. To develop effective vaccine against T. infection, we constructed a DNA encoding the rhoptry protein 17 (TgROP17) evaluated its immune protective efficacy acute infection in mice. The (p3×Flag-CMV-14-ROP17) was intramuscularly injected to BALB/c mice responses of vaccinated were determined. Compared control treated with empty vector or PBS,...
The disrupted organisation of the ryanodine receptors (RyR) and junctophilin (JPH) is thought to underpin transverse tubule (t-tubule) remodelling in a failing heart. Here, we assessed nanoscale these two key proteins human Recently, an advanced feature t-tubule identified large flattened t-tubules called t-sheets, that were several microns wide. Previously, reported heart, dilated up ~1 μm wide had increased collagen, hypothesised t-sheets would also be associated with collagen deposits....
Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease humans livestock. Effective vaccines are needed to limit prevent infection with Toxoplasma gondii. In this study, we evaluated immuno-protective efficacy recombinant gondii phosphoglycerate mutase 2 (rTgPGAM 2) against T. BALB/c mice. We report that mice nasally immunised rTgPGAM displayed significantly higher levels special IgG antibodies (including IgG1, IgG2a IgAs)...
The objective of these studies was to clarify the role Ca2+ in mechanism death evoked by human amylin (hA) islet β-cells. hA forms fibrils vitroand amyloid vivo. Here we show that pure synthetic aggregated solution, formed and cultured RINm5F β-cells a time-dependent (0-24 h) concentration-dependent (0-20 μM) manner. Dying cells underwent shrinkage nucleus, with clumping segregation chromatin into masses lay against nuclear envelope, internucleosomal DNA fragmentation. These therefore many...
Clara cell protein (CC16) is an anti-inflammatory protein, which expressed in the airway epithelium. It involved development of inflammatory diseases, including chronic obstructive pulmonary disease and asthma. However, exact molecular mechanism underlying its anti‑inflammatory action remains to be fully elucidated. The aim present study was define profiles effect CC16 lipopolysaccharide (LPS)‑treated rat tracheal epithelial (RTE) cells using shotgun proteomics. Protein extracts were...
Expression of the canine 180-kDa ribosome receptor p180 in yeast induces synthesis RER, and increases mRNAs secretory pathway proteins, protein secretion. To assess whether is a master regulator cell secretion mammalian cells, we stably expressed red fluorescent forms human variants p180DeltaR (no tandem repeats), p180R (26 full-length p180FR (54 repeats) containing different lengths repeat ribosome-binding domain rat pancreatic RINm5F islet beta-cells. All three localized exclusively to...
The objective of these studies was to clarify the role Ca2+ in mechanism death evoked by human amylin (hA) islet β-cells. hA forms fibrils vitroand amyloid vivo. Here we show that pure synthetic aggregated solution, formed and cultured RINm5F β-cells a time-dependent (0-24 h) concentration-dependent (0-20 μM) manner. Dying cells underwent shrinkage nucleus, with clumping segregation chromatin into masses lay against nuclear envelope, internucleosomal DNA fragmentation. These therefore many...
Kinectin (KNT) is a candidate membrane receptor for kinesin in the movement of intracellular organelles along microtubules. Isoforms KNT exist containing different combinations six small (residues 23-33) variable domains (vd) vd1-6 within C-terminus. Here we investigate role and its isoform KNTvd4(-) transport amylin insulin-containing secretory vesicles pancreatic islet beta-cell line RINm5F. lacks vd4 that forms kinesin-binding domain, hence cell an enigma. We report amylin-containing also...
Transverse (t)-tubule remodelling is a prominent feature of heart failure with reduced ejection fraction (HFrEF). In our previous research, we identified an increased amount collagen within the t-tubules HFrEF patients, suggesting fibrosis could contribute to t-tubules. this tested hypothesis in rodent model myocardial infarction induced that was treated anti-fibrotic pirfenidone. Confocal microscopy demonstrated loss border zone region infarct. This documented as reduction t-tubule...