A5067153938- Cancer-related molecular mechanisms research
- Sarcoma Diagnosis and Treatment
- Circular RNAs in diseases
- Autophagy in Disease and Therapy
- Management of metastatic bone disease
- RNA modifications and cancer
- Cancer Diagnosis and Treatment
- RNA Research and Splicing
- MicroRNA in disease regulation
- Hip and Femur Fractures
- Traditional Chinese Medicine Analysis
- Epigenetics and DNA Methylation
- Vascular Malformations and Hemangiomas
- Vascular Tumors and Angiosarcomas
- Bone health and osteoporosis research
- Bone and Joint Diseases
- Surgical site infection prevention
- Automated Road and Building Extraction
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Heterotopic Ossification and Related Conditions
- Head and Neck Surgical Oncology
- Dermatological and Skeletal Disorders
- Ferroptosis and cancer prognosis
- 3D Surveying and Cultural Heritage
Guangxi Medical University
2019-2025
Sichuan Agricultural University
2024
Tumor Hospital of Guangxi Medical University
2016-2023
McMaster University
2006
Osteosarcoma (OS), which occurs most commonly in adolescents, is associated with a high degree of malignancy and poor prognosis. In order to develop an accurate treatment for OS, deeper understanding its complex tumor microenvironment (TME) required. the present study, tissues were isolated from six patients then subjected single-cell RNA sequencing (scRNA-seq) using 10× Genomics platform. Multiplex immunofluorescence staining was subsequently used validate subsets identified by scRNA-seq....
In proteobacteria, genes whose expression is modulated in response to the external concentration of inorganic phosphate are often regulated by PhoB protein which binds a conserved motif (Pho box) within their promoter regions. Using position weight matrix algorithm derived from known Pho box sequences, we identified 96 putative regulon members regions contained one or more boxs Sinorhizobium meliloti genome. Expression these was examined through assays reporter gene fusions and comparison...
The novel loquat cultivar 'Chunhua No.1' (CH1) is a promising commercial cultivar. However, CH1 has texture characteristics different from those of common loquat, and its formation mechanism remains unclear. Here, we first identified the phenolic compounds parent ('Dawuxing', DWX) effect on formation. special presence stone cells explained flavor differences in CH1. Chlorogenic acid, neochlorogenic coniferyl alcohol were main high content was potential factor for rough Transcriptome reveals...
Stroke is a leading cause of morbidity and mortality worldwide, with modifiable risk factors being crucial for prevention efforts. Magnesium, an essential mineral involved in numerous physiological processes, linked to cardiovascular health. However, the relationship between magnesium status, assessed through Magnesium Depletion Score (MDS), stroke remains underexplored. This study aims investigate association MDS incidence, as well potential correlation all-cause disease (CVD) among US...
This study introduces a surgical technique involving the use of 3D-printed all-metal prostheses combined with mesh patches for treatment distal radial giant cell tumors, analyzing and evaluating midterm outcomes patients undergoing this treatment. The experience provides insights into application prosthesis replacement reconstructing defects. From January 2018 to 2021, our center treated five cases tumors using patches. Postoperative pain, range motion, grip strength were evaluated all...
Ankylosing spondylitis (AS) is a chronic inflammatory disease characterized with heterotopic ossification of the axis joints ligaments, resulting in joint disability. MicroRNAs (miRNAs) are regulators mRNAs that play crucial role AS pathological process. Here, we showed level miR-17-5p was significantly higher fibroblasts and ligament tissues from patients as compared to non-AS individuals. Knockdown derived exhibited decreased osteogenic differentiation ossification. On other hand,...
Background: PLK1, a typical PLK protein, is the main driver of cancer cell growth and proliferation. It an inhibitor protein kinases that currently being investigated in clinical studies. often used as tumor marker, high PLK1 expression correlates with poor prognosis cancer. Overexpression MYC hallmark many human cancers. modulates transcription thousands genes required to coordinate series cellular processes, including those essential for growth, proliferation, differentiation, self-renewal...
Microwave ablation has been used to treat bone tumors in extremities for more than 30 years. With improved recognition, updated microwave equipment, and expanded clinical application, recently widely tumors. To standardize the application of treatment limbs, research results experience involving use limbs have summarized, a guideline designed. This is aimed at providing reliable basis indications, preoperative evaluation decision‐making, perioperative treatment, complications, other issues...
Osteosarcoma (OS) is characterized by rapid growth and early metastasis. However, its mechanism remains unclear. N6-methyladenosine (m6A) modification regulatory factors play essential roles in most cancers, including OS. In this study, we screened out 21 m6A modifiers using the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database, followed identification of critical methylation modifiers. The results revealed that expression levels three regulators, namely...
Caveolin-1 (CAV-1), which is an oncoprotein and a tumor suppressor, highly expressed in normal osteoblasts. Although researchers have investigated its role human osteosarcoma, the mechanism of caveolin-1 action osteosarcoma remains unknown. In present study, Saos-2 U-2 OS cells were cultured with continuous induction protocol gradually increasing Taxol concentration for 6 months to establish drug-resistant cell lines. CAV-1 expression levels detected via western blotting quantitative...
By examining and identifying circulating tumor cell (CTC) counts subtypes of peripheral blood in osteosarcoma patients, we evaluated the relationship between CTCs characteristics as well CTC changes after neoadjuvant chemotherapy surgery.CanPatrol™ technology was used to detect before treatment 32 patients. Peripheral samples from 10 healthy volunteers were included controls examined for presence CTCs.Of detected 30 patients treatment, average count 14.06±9.08. No volunteers. The divided...
Purpose: The long noncoding RNA CCDC144NL antisense 1 (CCDC144NL-AS1) exhibits important functions in gastric cancer. In this study, we aimed to investigate the roles of CCDC144NL-AS1 modulating phenotype osteosarcoma (OS) cells vitro and vivo elucidate its underlying mechanisms. Methods: Reverse transcription quantitative polymerase chain reaction (PCR) was performed determine expression level OS tissues cell lines. proliferation, apoptosis, migration, invasion as well tumor growth were...
Anoikis resistance is a crucial step in the process of tumor metastasis. This determines whether cells will survive when they become detached from extracellular matrix. However, specific mechanism to bypass anoikis and resistant remains be elucidated. The present study aimed determine internal bypassing through comparison human osteosarcoma cell lines with normal lines. High activating transcription factor 4 (ATF4) myelocytomatosis oncogene (MYC) expression levels were observed MG‑63 U‑2 OS...
Melanoma is a highly malignant skin tumour, and one of the most rapidly growing tumors in recent years. According to statistics, morbidity cancer increases with age, accounting for 1.6% new cases 0.6% deaths worldwide. has serious impact on society families, thus it great significance find biological markers related diagnosis treatment melanoma.To explore expression predictive value mir-489 mir-21 melanoma metastasis.A total 60 patients treated at our hospital from June 2017 December 2018...
Calycosin is reportedly evidenced with pharmacologically treating bone cells. However, the comprehensive anti-osteosarcoma (OS) mechanisms of calycosin have not been uncovered. By using a systemic method network pharmacology, present study aimed to reveal potential anti-OS biotargets and molecular played by calycosin. Moreover, human animal experiments were conducted verify core against OS. As results, all primary biotargets, biological processes, pathways OS revealed. Additionally, top 20...
The objective of the study was to assess safety and efficacy microwave ablation (MWA) for breast cancer thoracic metastasis.Twelve patients in our institution with a single lesion metastases received MWA invasive spine surgery from August 2014 November 2016. executed using system (2,450 MHz) at 40 W or 50 thermometers control end points. pathology confirmed through intraoperative biopsy before ablation. postoperative complications were recorded. followed up 1, 3 6 months contrast-enhanced...
The incidence rate of thoracic metastasis from breast cancer is increasing. Microwave ablation one type clinical therapy used to treat metastatic spine disease, although it can cause protein denaturation and immediate cell death, coagulative necrosis occur. Minimally invasive open decompression associated with lower rates surgical complications in comparison traditional surgery. Therefore, an alternative therapeutic option for spinal metastases. This study aimed assess the efficacy microwave...
MicroRNAs (miRs), a class of small non-coding RNAs, function as key regulators in gene expression through binding to the 3'‑untranslated region (UTR) their target mRNA, which further leads translational repression or mRNA degradation. Recently, miR‑138 has been found have tumor suppressive role variety human malignancies. However, exact regulating malignant phenotypes osteosarcoma (OS) remained be elucidated. In present study, reverse‑transcription PCR analysis showed that was markedly...
Autophagy is closely related to skin cutaneous melanoma (SKCM), but the mechanism involved unclear. Therefore, exploration of role autophagy-related genes (ARGs) in SKCM necessary.Differential expression (DEARGs) were first analysed. Univariate and multivariate Cox regression analyses used evaluate DEARGs prognosis SKCM. Further, levels prognosis-related verified by immunohistochemical (IHC) staining. Finally, gene set enrichment analysis (GSEA) was explore underlying molecular mechanisms...