A5022080695- Adipose Tissue and Metabolism
- Virus-based gene therapy research
- Stress Responses and Cortisol
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Pharmacological Effects and Assays
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Diet and metabolism studies
- Animal Virus Infections Studies
- Cholesterol and Lipid Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- Gut microbiota and health
- Pancreatitis Pathology and Treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- CAR-T cell therapy research
- Clinical Nutrition and Gastroenterology
- Receptor Mechanisms and Signaling
- SARS-CoV-2 detection and testing
- Retinoids in leukemia and cellular processes
Sun Yat-sen University
2018-2023
Shanxi Agricultural University
2021
Southern Medical University
2021
Nanfang Hospital
2021
Zhejiang University
2013-2020
Sir Run Run Shaw Hospital
2013-2020
National Yang Ming Chiao Tung University
2013
Nanjing Agricultural University
2012
First Affiliated Hospital Zhejiang University
2009
Stockholm University
1994-1999
The possible significance of the coexisting beta 1-, 2-, and 3-adrenoceptors in brown adipose tissue for thermogenic response was investigated. Oxygen consumption isolated hamster fat cells analyzed as a measure thermogenesis. Thermogenesis could be evoked not only by physiological agent norepinephrine but also BRL-37344 CGP-12177. No evidence biphasic inhibition curves found with either selective 1-antagonist ICI-89406, 2-antagonist ICI-118551, or 1/beta 2-nonselective beta-antagonist...
The relationship between cAMP levels and thermogenesis was investigated in brown fat cells from Syrian hamsters. Irrespective of whether the selective β3-, β2-, β1-agonists BRL 37344, salbutamol, dobutamine or physiological agonist norepinephrine used to stimulate cells, increases were mediated via β3-receptor, as thermogenic effects. However, "thermogenesis per cAMP" much lower for agents other than norepinephrine. Similarly, forskolin, although more potent elevating cAMP, less inducing...
Tryptophan (Trp) metabolism through the kynurenine pathway (KP) is well known to play a critical function in cancer, autoimmune and neurodegenerative diseases. However, its role host-pathogen interactions has not been characterized yet. Herein, we identified that kynurenine-3-monooxygenase (KMO), key rate-limiting enzyme KP, quinolinic acid (QUIN), enzymatic product of KMO enzyme, exerted novel antiviral against broad range viruses. Mechanistically, QUIN induced production type I interferon...
The latent viral reservoir is the source of rebound after interruption antiretroviral therapy (ART) and major obstacle in eradicating human immunodeficiency virus-1 (HIV-1). In this study, arsenic class mineral, trioxide, clinically approved for treating acute promyelocytic leukemia, demonstrated to reactivate provirus CD4+ T cells from HIV-1 patients Simian virus (SIV)-infected macaques, without significant systemic cell activation inflammatory responses. a proof-of-concept study using...
To examine if acclimation of rats to cold led alterations in the coupling between different β-receptor subtypes and thermogenesis brown fat cells, we investigated adrenergic response patterns cells isolated from warm-acclimated (28°C) cold-acclimated (4°C) rats. In rats, relative affinities (EC 50 ) for agonists (isoprenaline, BRL-37344, norepinephrine, CGP-12177, dobutamine, salbutamol) were those expected their interaction with a β 3 -receptor. The norepinephrine was competitively...
Aims Soluble guanylyl cyclase (sGC) is a key modulator in the regulation of vascular tone. However, its role and involving mechanism cholesterol metabolism macrophages atherosclerosis remain unclear. Methods Oil red O staining, Dil-oxidized low-density lipoprotein (oxLDL)-binding assay efflux were performed biology foam cells. Levels cytokines or intracellular lipid evaluated by ELISA colorimetric kits. Expression gene protein was determined quantitative real-time PCR Western blotting....
Though immune correlates for protection are still under investigation, potent cytotoxic T lymphocyte responses desirable an ideal HIV-1 vaccine. PD-1 blockade enhances SIV-specific CD8+ cells. However, little information has been reported about how it affects the immunogenicity and of prophylactic SIV vaccines in nonhuman primates. Here, we show that during vaccination substantially improved protective efficacy challenged macaques. The pathway was blocked using a monoclonal antibody specific...
Abstract Porcine reproductive and respiratory syndrome (PRRS) induces disease failure accompanied by gastroenteritis-like symptoms. The mechanism of intestinal barrier injury caused PRRSV infection in piglets has yet to be investigated. An vivo PRRSV-induced model was established 30-day-old the intramuscular injection 2 mL 10 4 TCID 50 /mL for 15 days. Observations replication histology were conducted lungs intestine, goblet cell counts, relative MUC2 mRNA expression, tight junction protein,...
To examine the significance of brown adipose tissue for thermogenic response to glucagon, we injected glucagon intraperitoneally into rats (that have glucagon-sensitive fat cells) and hamsters glucagon-insensitive cells). Although a injection was apparently observed in rats, this not augmented by cold acclimation dose dependent. Similar observations were made hamsters. The could be fully blocked prior β-adrenergic blocker propranolol. Thus no direct demonstrated, due vehicle injection....
Deeply understanding the virus-host interaction is a prerequisite for developing effective anti-viral strategies. Traditionally, transporter associated with antigen processing type 1 (TAP1) critical presentation to regulate adaptive immunity. However, its role in controlling viral infections through modulating innate immune signaling not yet fully understood. In present study, we reported that TAP1, as product of interferon-stimulated genes (ISGs), had broadly antiviral activity against...
The persistence of cells latently infected with HIV-1, named the latent reservoir, is major barrier to HIV-1 eradication, and formation maintenance reservoir might be exacerbated by activation immunoinhibitory pathway dysfunction CD8+ T during infection. Our previous findings demonstrated that prophylactic vaccination combined PD-1 blockade generated distinct immune response profiles conferred effective control highly pathogenic SIVmac239 infection in rhesus macaques. However, our surprise,...
Deeply understanding virus-host interactions is a prerequisite for developing effective strategies to control frequently emerging infectious diseases, which have become serious challenge global public health. The type I interferon (IFN)-mediated JAK/STAT pathway well known playing an essential role in host antiviral immunity, but the exact regulatory mechanisms of various IFN-stimulated genes (ISGs) are not yet fully understood. We herein reported that SerpinA5, as novel ISG, played...
Arotinolol, a clinically used alpha/beta-adrenergic blocker, has been demonstrated to be an anti-obesity agent. The effect of arotinolol was suggested the result direct activation thermogenesis in brown-fat cells. We tested ability stimulate (oxygen consumption) isolated cells and intact animals. Arotinolol stimulated from mouse hamster. A relatively low sensitivity beta-adrenergic antagonist propranolol (pK(B) approximately 6) indicated that interacted with beta3-adrenergic receptor. On...
Abstract Epitope-based vaccination might play an important role in the protective immunity against Japanese encephalitis virus (JEV) infection. The purpose of study is to evaluate immune characteristics recombinant MVA carrying multi-epitope gene JEV (rMVA-mep). synthetic containing critical epitopes (B-cell, CTL and Th) was cloned into eukaryotic expression vector pGEM-K1L, rMVA-mep prepared. BALB/c mice were immunized with different dosages purified responses determined form response JEV,...
We evaluated the immunogenicity and protective ability of a chimpanzee replication-deficient adenovirus vectored COVID-19 vaccine (BV-AdCoV-1) expressing stabilized pre-fusion SARS-CoV-2 spike glycoprotein in golden Syrian hamsters. Intranasal administration BV-AdCoV-1 elicited strong humoral cellular immunity animals. Furthermore, vaccination prevented weight loss, reduced infectious virus titers lungs as well lung pathology provided protection against live challenge. In addition, there was...
Two Autographa californica nucleopolyhedrovirus (AcMNPV) encoded miRNAs, AcMNPV-miR-1 and AcMNPV-miR-3, have been reported by us in 2013 2019, respectively. Here, we present an integrated investigation of AcMNPV-encoded which include the above two miRNAs three additional newly identified miRNAs. Six candidate were predicted through small RNA deep sequencing bioinformatics, which, five validated. Three are located opposite coding sequences, other sequences viral genes. Targets both virus host...
The ability of the Beta 1 /Beta 2 partial agonist carteolol to act as an on 3 -adrenergic receptors was investigated by studying its stimulatethermogenesis (oxygen consumption) in brown fat cells isolated from hamsters. Carteolol wasable induce thermogenesis, with EC 50 5 µM, but it only a partial(40%) agonist. D,L-Propranolol had pK B 5.1 anantagonist against this system, indicating that effect probablymediated via -receptors. Also mouse and rat cells, withEC values around µM. Being...
Arotinolol, a clinically used α/β-adrenergic blocker, has been demonstrated to be an anti-obesity agent. The effect of arotinolol was suggested the result direct activation thermogenesis in brown-fat cells. We tested ability stimulate (oxygen consumption) isolated cells and intact animals. Arotinolol stimulated from mouse hamster. A relatively low sensitivity β-adrenergic antagonist propranolol (pK B [Formula: see text] 6) indicated that interacted with β 3 -adrenergic receptor. On...