A5017428835- Birth, Development, and Health
- Fatty Acid Research and Health
- Pregnancy and preeclampsia studies
- Mosquito-borne diseases and control
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Gestational Diabetes Research and Management
- Nutritional Studies and Diet
- Antioxidant Activity and Oxidative Stress
- Adenosine and Purinergic Signaling
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Eicosanoids and Hypertension Pharmacology
- Enzyme Catalysis and Immobilization
- Viral Infections and Vectors
- Erythrocyte Function and Pathophysiology
- Infant Nutrition and Health
- Endoplasmic Reticulum Stress and Disease
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- Adipose Tissue and Metabolism
- HIV Research and Treatment
- Infectious Encephalopathies and Encephalitis
- Breastfeeding Practices and Influences
Universidade Federal do Rio de Janeiro
2016-2025
University of Cambridge
2019-2022
Instituto de Nutricion e Higiene de los Alimentos
2021
Universidade do Estado do Rio de Janeiro
2021
National Institute of Science and Technology for Structural Biology and Bioimaging
2017
3-BrPA (3-bromopyruvate) is an alkylating agent with anti-tumoral activity on hepatocellular carcinoma. This compound inhibits cellular ATP production owing to its action glycolysis and oxidative phosphorylation; however, the specific metabolic steps mechanisms of in human carcinomas, particularly effects mitochondrial energetics, are poorly understood. In present study it was found that incubation HepG2 cells a low concentration for short period (150 μM 30 min) significantly affected both...
Abstract Aim The current study investigated the impact of maternal obesity on placental phenotype in relation to fetal growth and sex. Methods Female C57BL6/J mice were fed either a diet high fat sugar or standard chow diet, for 6 weeks prior to, during, pregnancy. At day 19 gestation, morphology mitochondrial respiration dynamics assessed using high‐resolution respirometry, stereology, molecular analyses. Results Diet‐induced increased rate small gestational age fetuses both sexes, blood...
The aim of this work was to determine whether placental endoplasmic reticulum (ER) stress may contribute the pathophysiology gestational diabetes mellitus (GDM) and test efficacy chemical chaperones antioxidant vitamins in ameliorating that a trophoblast-like cell line vitro.Placental samples were obtained from women suffering GDM normoglycaemic controls frozen immediately. Women with had 2 h serum glucose levels > 9.0 mmol/l following 75 g oral tolerance treated diet insulin when necessary....
Structured lipids (SL) represent a new generation of lipids, considered bioactive compounds. Medium-chain, oleic (18:1n-9), and medium-chain fatty acid (MCFA) structured (MOM-SL) were produced by acidolysis reaction in solvent-free medium with capric (10:0) lauric (12:0) free acids (FFAs) triolein or olive oil, using Yarrowia lipolytica lipase as biocatalyst. MCFAs rapidly incorporated into sn-1,3 SL reactions up until 30% incorporation efficiency SLs. The kinetics MCFA MOM-SL was influenced...
Modulation of the gut microbiota may help in treating obesity by improving host metabolic health. We aimed to evaluate effects probiotics or symbiotics on body weight and serum metabolite profile women with obesity. A double-blind, parallel, randomized, controlled clinical trial was conducted 32 adult mass index ranging from 30 34.9 kg m-2. Volunteers followed a low-energy diet were subjected 8 weeks intervention: probiotic group (PG - Bifidobacterium lactis UBBLa-70, n = 10), symbiotic (SG...
Background Tumor cells are characterized by accelerated growth usually accompanied up-regulated pathways that ultimately increase the rate of ATP production. These can suffer metabolic reprogramming, resulting in distinct bioenergetic phenotypes, generally enhancing glycolysis channeled to lactate In present work we showed reprogramming means inhibitors histone deacetylase (HDACis), sodium butyrate and trichostatin. This treatment was able shift energy metabolism activating mitochondrial...
Glutamine is a conditionally essential amino acid that an important metabolic resource for proliferating tissues by acting as proteinogenic acid, nitrogen donor biosynthetic reactions and substrate the citric or tricarboxylic cycle. The human immunodeficiency virus type 1 (HIV-1) productively infects activated CD4+ T cells are known to require glutamine proliferation carrying out effector functions. As virus, HIV-1 furthermore entirely dependent on host metabolism support its replication. In...
Cancer cells demand high ATP provisions to support proliferation, and targeting of energy metabolism is a good strategy increase their sensitivity treatments. In Brazil, wine manufacture expanding, increasing the amount pomace that produced. We determined phenolic composition antioxidant properties dark skin Grape Pomace Extract its effects on redox state in human hepatocarcinoma HepG2 cells. The material methods used represented industrial process since derived from white production extract...
The metabolic resources crucial for viral replication are provided by the host. Details of mechanisms which viruses interact with host metabolism, altering and recruiting high free-energy molecules their own replication, remain unknown. Sindbis virus, prototype most widespread alphavirus, causes outbreaks arthritis in humans serves as a model study pathogenesis neurological diseases induced alphaviruses mice. In this work, respirometric analysis was used to evaluate effects virus infection...
ABSTRACT Dengue, due to its global burden, is the most important arthropod-borne flavivirus disease, and early detection lowers fatality rates below 1%. Since metabolic resources crucial for viral replication are provided by host cells, of changes in profile associated with disease pathogenesis could help identification markers prognostic diagnostic importance. We applied 1 H nuclear magnetic resonance exploratory metabolomics study longitudinal plasma metabolites a cohort Recife, Brazil. To...
Our aims were to investigate vitamin A and E status during lactation the determinants of breast milk content for appropriate nutrition infant in a study with nursing Brazilian women. We hypothesized that both inadequate intake lipoprotein distribution could have an impact on their levels from early- mid-lactation. Nineteen adult lactating women participated this longitudinal observational study, which dietary records, blood mature samples collected analysis E, carotenoids (2nd 4th week)...
Casein kinase 2 (CK2) plays a critical role in the proliferation and apoptosis of cancer cells. Resveratrol is bioactive compound with anticancer anti-inflammatory effects. This study investigated pro-oxidant cytotoxic effects resveratrol association inhibition CK2 activity on human breast carcinoma cells MCF-7. We showed that TBB, an inhibitor CK2, decreased cell viability concentration dependent manner IC50 value 238 µM 106 after 24 h, treatment, respectively. TBB by 1.6 1.4-fold,...
The quality of dietary lipids in the maternal diet can programme offspring to diseases later life. We investigated whether intake palm oil or interesterified fat, substitutes for trans-unsaturated fatty acids (FA), induces metabolic changes adult offspring. During pregnancy and lactation, C57BL/6 female mice received normolipidic diets containing partially hydrogenated vegetable fat rich (TG), (PG), (IG) soyabean (CG). After weaning, male from all groups control until day 110. Plasma glucose...
We combined 1H NMR metabolomics with functional and molecular biochemical assays to describe the metabolic changes elicited by vitamin D in HEK293T, an embryonic proliferative cell line adapted high-glucose concentrations. Activation of polyol pathway, was most important consequence exposure high glucose concentration, resembling cells exposed hyperglycemia. Vitamin induced alterations HEK293T metabolism, including a decrease sorbitol, glycine, glutamate, guanine. modulated glycolysis...
Thyroid hormones regulate adult metabolism partly through actions on mitochondrial oxidative phosphorylation (OXPHOS). They also affect neurological development of the brain, but their role in cerebral OXPHOS before birth remains largely unknown, despite increase energy demand during neonatal period. Thus, this study examined prepartum hypothyroid fetal sheep. Using respirometry, Complex I (CI), II (CII), and combined CI&CII capacity were measured cerebellum cortex at 128 142 days...
The rise in prevalence of obesity women reproductive age developed and developing countries might propagate intergenerational cycles detrimental effects on metabolic health. Placental lipid metabolism is disrupted by maternal obesity, which possibly affects the life-long health offspring. Here, we investigated placental with pre-gestational as a sole pregnancy complication compared it to responses lean women. Open profile targeted lipidomics were used assess lipids oxidised products...
Brazil has the second-highest COVID-19 death rate worldwide, and Rio de Janeiro is among states with highest in country. Although vaccine coverage been achieved, it anticipated that will transition into an endemic disease. It concerning molecular mechanisms underlying clinical evolution from mild to severe disease, as well leading long COVID-19, are not yet fully understood. NMR MS-based metabolomics were used identify metabolites associated pathophysiology disease outcome. Severe cases (n =...