It is well established that cancer cells depend upon aerobic glycolysis to provide the energy they need survive and proliferate. However, anti-glycolytic agents have yielded few positive results in human patients, part due dose-limiting side effects. Here, we discovered unexpected anti-cancer efficacy of Polydatin (PD) combined with 2-deoxy-D-glucose (2-DG), which a compound inhibits glycolysis. We demonstrated two breast cell lines (MCF-7 4T1) combination treatment PD 2-DG induced apoptosis...

Hyperoside (quercetin 3-o-β-d-galactopyranoside) is one of the flavonoid glycosides with anti-inflammatory, antidepressant, and anti-cancer effects. But it remains unknown whether had effects on breast cancer. Here, different concentrations hyperoside were used to explore its therapeutic potential in both cancer cells subcutaneous homotransplant mouse model. CCK-8 wound healing assays showed that viability migration capability Michigan Cancer Foundation-7 (MCF-7) 4T1 inhibited by hyperoside,...

Acute lung injury (ALI) is a life-threatening inflammatory disease owing to the lack of specific and effective therapies. Oridonin (Ori) an active diterpenoid isolated from Rabdosiarubescens (R.rubescens) that has been shown possess broadspectrum pharmacological properties including anti-inflammatory, antitumour, antioxidative neuroregulatory effects. However, its potential protective mechanism in ALI not well characterized. In this study, we demonstrated Ori reduces mortality mice with...

Abstract As a modern biomedical therapeutic modality, sonodynamic therapy (SDT) presents unique advantages, including superior tissue penetration capability, temporal–spatial controllability, and negligible side effects. However, the bottlenecks of most organic sonosensitizers are their short emission wavelengths, strong phototoxicity, unsatisfactory SDT effect, which undermines precise fluorescence imaging‐guided in vivo. Here, long‐wavelength emissive mitochondria‐targeted...

Endometritis is one of the most common causes infertility in dairy cows, and histopathologically characterized by inflammation damage endometrial epithelium. Interferon-tau (IFN-τ) a novel type I interferon secreted ruminant trophoblast cells with low cytotoxicity even at high doses. Previous studies suggested that IFN-τ plays an important role inflammation. However, mechanisms whereby may modulate inflammatory responses bovine endometrium are unknown. In present study, primary epithelial...

The internalization of S. aureus in bMECs is a major pathogenic mechanism leading to mastitis, causing significant economic losses the dairy industry. Numerous plants contain Lut, natural flavonoid with anti-inflammatory and antioxidant properties. However, little known about Lut’s ability reduce inflammation caused by bMECs. This research aimed evaluate which Lut reduces aureus-induced Through GO KEGG enrichment analysis, researchers analyzed differentially expressed genes infected NCBI GEO...

Breast cancer is one of the most lethal cancers in world.The fight against breast has also become a major task for medical workers.MicroRNAs (miRNAs) are often aberrantly expressed diverse and involved progression metastasis.Many studies have found that miRNAs can act as oncogenes or tumor suppressor genes.Here, we show miR-433 significantly decreased cells.In addition, demonstrate effects on cell apoptosis, migration proliferation an attempt to elucidate mechanism action miR-433.Moreover,...

Abstract Endometritis is a postnatal reproductive disorder disease, which leads to great economic losses for the modern dairy industry. Emerging evidence indicates that microRNAs (miRNAs) play pivotal role in variety of diseases and have been identified as critical regulators innate immune response. Recent miRNome profile analysis revealed an altered expression level miR‐148a cows with endometritis. Therefore, present study aims investigate regulatory response involved endometritis estimate...

Mastitis is a common disease in the dairy industry that causes huge economic losses worldwide. Exosomes (carrying proteins, miRNA, lncRNA, etc.) play vital role regulation of immune response. lncRNA can variety regulatory roles by combining with protein, RNA, and DNA. The expression mRNA exosomes derived from bovine mammary epithelial cells infected S. aureus rarely understood. To explore this issue, RNA sequencing analysis was performed on aureus-infected noninfected MAC-T cells. Analysis...

Mastitis, caused by a variety of pathogenic microorganisms, seriously threatens the safety and economic benefits dairy industry. Vitexin, flavone glucoside found in many plant species, has been widely reported to have antioxidant, anti-inflammatory, antiviral, anticancer, neuroprotective, cardioprotective effects. However, few studies explored effect vitexin on mastitis. This study is aimed at exploring whether antioxidant anti-inflammatory functions can improve Staphylococcus aureus-induced...

Abstract Sodium selenite (SSE), a source of inorganic selenium, has been widely used as clinical cancer treatment, but the precise molecular mechanisms SSE remain to be elucidated. Our in vitro experiments have confirmed that treatment causes transient increase intracellular reactive oxygen species (ROS) levels, resulting inhibition nuclear transcription factor‐κB (NF‐κB) signaling and p65 factor kappa light polypeptide gene enhancer B‐cells inhibitor, alpha phosphorylation levels 4T1 cells....

Abstract Breast cancer is a common malignancy that highly lethal with poor survival rates and immature therapeutics urgently needs more effective efficient therapies. MicroRNAs are intrinsically involved in different remedies, but their mechanism breast has not been elucidated for prospective treatment. The function of microRNA‐188‐5p (miR‐188) have thoroughly investigated cancer. In our study, we found the expression miR‐188 tissues was obviously reduced. Our findings also revealed abnormal...

Alpinetin, the main active ingredient in Chinese medicinal herb Alpinia katsumadai Hayata, has been found to have anticancer activity. However, therapeutic efficacy of signalling cascades modulated by alpinetin remains unknown. Here, we showed that provoked mitochondria-associated apoptosis a dose-dependent manner breast cancer cells. Mechanistic investigations revealed dampens hypoxia-inducible factor-1α (HIF-1α) due lack NF-κB activation through reduced mitochondrial reactive oxygen...

2-Deoxyglucose (2-DG) can be used in antitumour research by inhibiting glycolysis and promoting the endoplasmic reticulum stress (ERS) pathway, but its clinical application is restricted due to dose-limiting side effects survival chance for cancer cells protective autophagy. Therefore, our explored whether combination of hydroxychloroquine (HCQ), an FDA-approved autophagy drug, 2-DG a promising therapeutic strategy. Here, we report that HCQ combined with further inhibit viability migration...

Pregnancy failure in the first trimester of cows significantly impacts efficiency dairy industry. As a type I interferon exclusively to ruminants, IFN-τ plays key role maternal recognition and immune tolerance fetuses. Macrophages are most common cells within ruminant endometrium. Nevertheless, deeply analyzing mechanisms regulating macrophage polarization still needs further study. In this study, notable decline bta-miR-30b-5p expression via increase SOCS1 was observed uterine tissues...

Oxidative stress is a significant factor affecting reproductive efficiency in dairy cows, contributing to conditions such as endometritis that impair fertility and milk production. This study investigates the molecular mechanisms by which bta-let-7d modulates oxidative responses induced potassium permanganate (KMnO₄) bovine endometrial epithelial cells (BEECs). Using KMnO₄ induce stress, we observed increases reactive oxygen species (ROS) malondialdehyde (MDA) levels, accompanied decreased...

Potassium permanganate (KMnO4) is a commercially available antiseptic used in bovine intrauterine lavage to manage postpartum infections. Lipopolysaccharides (LPS) are well-studied for their ability induce inflammation and oxidative stress. While KMnO4 known cause significant irritation, stress, toxicity uterine tissues, its transcriptional impact potential inducing similar molecular damage as LPS have not been fully explored. In this study, we induced stress the tissues of Sprague–Dawley...

Abstract Bovine embryo resorption is one of the key factors restricting reproductive efficiency in dairy cattle, which mainly occurs early stage maternal recognition pregnancy (MRP), causing substantial economic losses to industry. Macrophages, most numerous endometrial immune cells cows during pregnancy, are critical developing maternal-fetal tolerance. However, mechanism their action on interface remains unknown. MicroRNAs have important roles tolerance and macrophage polarization, but...

Reproductive disorders in dairy cows represent a significant challenge to the advancement of industry. Pregnancy success is closely related mechanism immune tolerance, with PD-1/PD-L1 signaling pathway playing role regulation, which associated tolerance and pregnancy maintenance. MicroRNAs can regulate pivotal molecules within pathway, physiological activities, disease processes. p65 PD-L1 expression was significantly increased early pregnant uterine epithelium. In contrast, homogeneous...

Oxidative stress is a significant factor affecting reproductive efficiency in dairy cows, contributing to conditions such as endometritis that impair fertility and milk production. This study investigates the molecular mechanisms by which bta-let-7d modulates oxidative responses induced potassium permanganate (KMnO4) bovine endometrial epithelial cells (BEECs). Using KMnO4 induce stress, we observed increases reactive oxygen species (ROS) malondialdehyde (MDA) levels, accompanied decreased...

Selenium (Se) deficiency disrupts intracellular REDOX homeostasis and severely deteriorates immune anti-inflammatory function in high-yielding periparturient dairy cattle. To investigate the damage of extracellular vesicles derived from Se-deficient MAC-T cells (SeD-EV) on normal mammary epithelial cells, an vitro model Se was established. produced many ROS, promoting apoptosis release inflammatory factors. Extracellular were successfully isolated by ultrahigh-speed centrifugation identified...