A5014821886- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Algal biology and biofuel production
- Yersinia bacterium, plague, ectoparasites research
- Salmonella and Campylobacter epidemiology
- Phagocytosis and Immune Regulation
- Inflammasome and immune disorders
- Vibrio bacteria research studies
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Ion Channels and Receptors
- Ion channel regulation and function
- Immune Response and Inflammation
- Heat shock proteins research
- Neonatal Respiratory Health Research
- Viral gastroenteritis research and epidemiology
- Hearing, Cochlea, Tinnitus, Genetics
- Biocrusts and Microbial Ecology
- Viral Infections and Vectors
- Immune cells in cancer
- Photoreceptor and optogenetics research
- Bacillus and Francisella bacterial research
Stanford University
2012-2015
Ruhr University Bochum
2008-2014
Ludwig-Maximilians-Universität München
2007
Expression of all Yersinia pathogenicity factors encoded on the virulence plasmid, including yop effector and ysc type III secretion genes, is controlled by transcriptional activator LcrF in response to temperature. Here, we show that a protein- RNA-dependent hierarchy thermosensors induce synthesis at body Thermally regulated transcription lcrF modest mediated thermo-sensitive modulator YmoA, which represses from single promoter located far upstream yscW-lcrF operon moderate temperatures....
Murine NLR family, apoptosis inhibitory protein (Naip)1, Naip2, and Naip5/6 are host sensors that detect the cytosolic presence of needle rod proteins from bacterial type III secretion systems flagellin, respectively. Previous studies using human-derived macrophage-like cell lines indicate human macrophages sense protein, but not flagellin. In this study, we show primary readily Infection with Salmonella elicits robust death IL-1β is dependent on We flagellin detection requires a full-length...
Despite the development of effective therapies, a substantial proportion asthmatics continue to have uncontrolled symptoms, airflow limitation, and exacerbations. Transient receptor potential cation channel member A1 (TRPA1) agonists are elevated in human asthmatic airways, rodents, TRPA1 is involved induction airway inflammation hyperreactivity. Here, discovery early clinical GDC-0334, highly potent, selective, orally bioavailable antagonist, described. GDC-0334 inhibited function on smooth...
Structured RNA regions are important gene control elements in prokaryotes and eukaryotes. Here, we show that the mRNA of a cyanobacterial heat shock contains built-in thermosensor critical for photosynthetic activity under stress conditions. The exceptionally short 5′-untranslated region is comprised single hairpin with an internal asymmetric loop. It inhibits translation Synechocystis hsp17 transcript at normal growth conditions, permits initiation conditions shuts down Hsp17 production...
Significance Vibrio cholerae causes the life-threatening diarrheal disease cholera. These bacteria live in aquatic environments and are introduced into humans through contaminated water sources. The express virulence factors upon entry human gastrointestinal tract. present study shows that factor expression is controlled by shift temperature to 37 °C, an RNA thermometer controls translation of major regulator protein ToxT. This simple mechanism allows maximal within mammalian host may be...
The initiation, progression and transmission of most bacterial infections is dependent upon the ability invading pathogen to acquire iron from each varied environments encountered during course a natural infection. In total, 95% within human body complexed heme, making heme potentially rich source host-associated nutrient for bacteria. As only host, pathogenic bacteria often regulate synthesis utilization factors such that production maximal under environmental conditions. This study...
Gene expression in chloroplasts is regulated mainly at the posttranscriptional level. In green alga Chlamydomonas reinhardtii, synthesis of D2 protein (PsbD), which rate-determining subunit for assembly photosystem II, depends on RNA stability factor Nac2. addition, binding RBP40 has been implicated translational control via a U-rich element 5' untranslated region (5'UTR) psbD mRNA. Here, we report identification gene based mass spectrometric analysis its purified product. Unexpectedly, this...
Structured RNAs with fundamental sensory and regulatory potential have been discovered in all kingdoms of life. Bacterial RNA thermometers are located the 5'-untranslated region certain heat shock virulence genes. They regulate translation by masking Shine-Dalgarno sequence a temperature-dependent manner. To engineer RNA-based thermosensors, we used combination computer-based rational design vivo screening. After only two rounds selection, several that at least as efficient natural were...
It is well understood that the adaptive immune response to infectious agents includes a modulating suppressive component as an activating component. We now show very early innate also has immunosuppressive Infected cells upregulate CD47 "don't eat me" signal, which slows phagocytic uptake of dying and viable downstream antigen-presenting cell (APC) functions. A mimic acts essential virulence factor encoded by all poxviruses, but expression on infected was found be upregulated even pathogens,...
Sensing and responding to environmental signals is critical for bacterial pathogens successfully infect persist within hosts. Many sense temperature as an indication they have entered a new host must alter their virulence factor expression evade immune detection. Using secondary structure prediction, we identified RNA thermosensor (RNAT) in the 5’ untranslated region (UTR) of tviA encoded by typhoid fever-causing bacterium Salmonella enterica serovar Typhi ( S . Typhi). Importantly,...
Cyanobacteria constitute a heterogeneous phylum of oxygen-producing, photosynthetic prokaryotes. They are susceptible to various stress conditions like heat, salt, or light stress, all inducing the cyanobacterial heat shock response (HSR). Cyanobacterial small proteins (sHsps) known preserve thylakoid membrane integrity under conditions, thereby protecting photosynthesis machinery. In Synechocystis sp PCC 6803, synthesis sHsp Hsp17 is regulated by an RNA thermometer (RNAT) in 5'-untranslated...
Abstract Sensory neurons of the vagal ganglia (VG) innervate lungs and play a critical role in maintaining airway homeostasis. However, specific VG that lungs, mechanisms by which these sense respond to insults, are not well understood. Here, we identify subpopulation lung-innervating defined their expression Tmc3 . Single cell transcriptomics illuminated several subpopulations Tmc3+ sensory neurons, revealing distinct Piezo2 - Trpv1 -expressing subclusters. Furthermore, deficiency leads...
After this article [1] was published, concerns were raised about the reported western blot results.Specifically:• There appear to be vertical discontinuities between lanes in following panels: Figs 1 (after lane 3), 4A 2), 7C 1) and 7D 2).For each figure panel, discontinuity reflects where nonadjacent image fragments from same exposure spliced together preparing figure.Figs 1, 4 7 below have been updated clarify that panels represent blots, original images are provided S1-S4 Files.• appears...