A5100339639- Angiogenesis and VEGF in Cancer
- Venous Thromboembolism Diagnosis and Management
- Lymphatic System and Diseases
- Ecology and Conservation Studies
- Wound Healing and Treatments
- Cancer Treatment and Pharmacology
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Cardiac and Coronary Surgery Techniques
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Pancreatic function and diabetes
- Gastric Cancer Management and Outcomes
- Hemoglobin structure and function
- Cancer, Lipids, and Metabolism
- Natural Antidiabetic Agents Studies
- Plant tissue culture and regeneration
- Lipid metabolism and disorders
- Chemotherapy-induced cardiotoxicity and mitigation
- Histone Deacetylase Inhibitors Research
- Cancer Mechanisms and Therapy
- Coronary Interventions and Diagnostics
- Protease and Inhibitor Mechanisms
- Meat and Animal Product Quality
Nanjing Drum Tower Hospital
2020-2024
Second Affiliated Hospital of Soochow University
2009-2018
Soochow University
2010-2018
Southern Medical University
2018
Luliang University
2006
Endothelial progenitor cells (EPCs) contribute to recanalization of deep vein thrombosis (DVT). This study aimed detect miRNA expression profiles in EPCs from patients with DVT and characterize the role dysfunction. was isolated control subjects, were compared screen differential miRNAs. The candidate miRNAs confirmed by RT-PCR analysis. targets identified bioinformatics analyses, luciferase reporter assay gene analyses. apoptosis, migration tube formation examined flow cytometry, transwell...
The aim of the present study was to investigate effect metformin on endothelial progenitor cell (EPC) migration and explore possible mechanisms. EPCs were treated with metformin, evaluated by wound healing Matrigel invasion assays. We also examined expression levels MMP-2 MMP-9 in or without treatment via RT-PCR western blot analysis, activities under different conditions zymography. Moreover, we assessed AMPK/mTOR/autophagy pathway Metformin significantly downregulated matrix...
We investigated the protective effects and mechanism of action metformin on high glucose-induced smooth muscle cell proliferation migration.Vascular cells (VSMCs) were subjected to a series concentrations (0-10 mM) metformin.CCK-8, wound healing, transwell assays performed.Correlations between concentration high-mobility group box 1 (HMGB1) miR-142-3p levels assessed.In addition, mimic siRNA used investigate VSMC migration in presence or absence metformin.In high-glucose condition, decreased...
Abnormal vascular smooth muscle cell (VSMC) proliferation has an important role in the pathogenesis of both atherosclerosis restenosis and hypertension. Vascular endothelial growth factor (VEGF) been shown to stimulate VSMC proliferation. In addition, angiogenesis is one hallmarks cancerous growth. VEGF key modulator for initial stages that acts through endothelial‐specific receptor tyrosine kinases (VEGFRs). VEGFR‐2 blockage a good approach suppression angiogenesis. order discover novel TK...
BACKGROUND:Endothelial progenitor cells (EPCs) were found to be a potential therapeutic choice for low extremity deep vein thrombosis. The aim of our research was investigate the effect resveratrol (RSV) on EPCs that may promote thrombus resolution and its pathway. MATERIAL AND METHODS:EPCs pretreated with RSV migration; angiogenesis evaluated ex vivo. Expression miR-138 focal adhesion kinase (FAK) also tested. A murine model venous thrombosis developed as an in vivo model. effects treatment...
Background: Preclinical and clinical studies indicated that endothelial progenitor cells (EPCs) enhanced blood vessel formation in many situations. However, whether transplantation of EPCs would enhance chronic venous thrombus recanalization resolution is unknown. Methods: Mononuclear were isolated from bone marrow immature rats by density gradient centrifugation, cultured, then transplanted into inferior vena cava with experimentally induced thrombi. Vascular growth factor (VEGF), basic...
Angiogenesis is a physiological procedure during which the new blood vessels develop from pre-existing vessels. Uncontrolled angiogenesis related to various diseases including cancers. Clinical inhibition of undesired still under investigation. We utilized nicotinic acid, family member B-vitamin niacin (vitamin B3) that has been used in prevention and treatment atherosclerosis or other lipid-metabolic disorders, treat human umbilical vein endothelial cells (HUVECs) chick chorioallantoic...
Objective To observe the biological characteristics and precise role in angiogenesis of endothelial progenitor cells (EPCs).Methods Bone marrow-derived mononuclear were isolated cultured.EPCs identified by using immunohistochemistry,immunofluorescence confocal microscopy.Using methyl thiazol tetrazolium(MTT) method,three-dimensional culture flow cytometry,the early late EPCs compared.The features observed transmission electron microscopy.Results expressed cell surface antigen positive...
Objective To investigate the expression of vascular endothelial growth factor (VEGF)in ECV304 cells after transfection with Ad tissue plasminogen activator (t-PA) in vitro and signaling pathway involved. Methods The VEGF was detected mixed culture Adt-PA for24 h as group 1, compared that 2 (mixed for48 h) 3 ( for 48 h). phosphorylated extracellular regulated protein kinases (pERK) changes blocking phosphorylation ERK by PD98059 were examined. Results increased obviously groups 1 (P ＜0. 01 )....
Objective To investigate the effect of bone marrow derived endothelial progenitor cells (EPC)transplantation on microenvironments in a murine model chronic vein thrombosis.EPCs transplantation was evaluated whether it can up-regulate thrombus organization and recanalization associated cytokines(VEGF,ANG-1 MCP-1). Method EPCs from immature Wister rats' were isolated using Ficoll density gradient centrifugation,and cultured fibronectin-coated plate EGM-2M Vmedium.EPCs harvested 10th day,then...
Objective To study the effect of endothelial progenitor cells(EPCs) transplantation on chronic deep venous thrombosis.Methods Bone marrow-derived mouonuclear cells (BMMNCs) were isolated from rat bone marrow by ficoll and cultured with EGM-2MV medium.A model vein thrombosis was established partial ligation inferior vena cava intravenous injection thrombosin.Model rats randomly divided into three groups:A(n =25),EPCs group,1 ml 10~6 EPCs transplantation;B(n = 25),EGM-2MV medium...