A5100785117- Virus-based gene therapy research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Cancer Research and Treatments
- Poxvirus research and outbreaks
- interferon and immune responses
- Animal Virus Infections Studies
- Venous Thromboembolism Diagnosis and Management
- Autophagy in Disease and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related gene regulation
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Extracellular vesicles in disease
- Acute Myeloid Leukemia Research
- Cancer Immunotherapy and Biomarkers
- Cancer Mechanisms and Therapy
- Plant Virus Research Studies
Nanjing Drum Tower Hospital
2014-2024
Harbin Medical University
2015-2024
First Affiliated Hospital of Harbin Medical University
2015-2024
Argonne National Laboratory
2023
Weatherford College
2023
Genetic Analysis (Norway)
2023
Henan University Huaihe Hospital and Huaihe Clinical Institute
2016-2023
Xinjiang Medical University
2023
Wuhan University
2023
Zhongnan Hospital of Wuhan University
2023
Apoptin is a small molecular weight protein encoded by the VP3 gene of chicken anemia virus (CAV). It can induce apoptosis tumor cells and play anti-tumorigenic functions. In this study, we identified time-dependent inhibitory role apoptin on viability HCT116 cells. We also demonstrated that induces pyroptosis through cleaved caspase 3, with concomitant cleavage gasdermin E (GSDME) rather than GSDMD. GSDME knockdown switched apoptin-induced cell death from to in vitro. Furthermore, effect...
Efficient recruitment and angiogenesis of endothelial progenitor cells (EPCs) are critical during a thrombus event. However, the details EPC regulation have not been fully determined. The aim this study was to determine role long noncoding (lnc)RNA Wilms tumor 1 associated protein pseudogene (WTAPP1) in migration EPCs. EPCs were isolated from human peripheral blood characterized by flow cytometry, after which lentivirus-mediated lncRNA WTAPP1 overexpression knockdown performed. Scratch...
Silver nanoparticles (AgNPs) are the most commonly used engineered nanomaterials in commercialized products because of their antimicrobial activity. Previously, we have shown that polyvinylpyrrolidone (PVP)-coated AgNPs an anti-leukemia effect against human myeloid leukemia cells; however, whether able to trigger autophagy normal hematopoietic cells and role AgNP-induced cytotoxicity remain unclear. In current study, observed were taken up by murine pro-B (Ba/F3), then promoted accumulation...
Ulcerative colitis is one of the most common types inflammatory bowel disease and multifactorial relapsing. 6-Gingerol, a component gingerols extracted from ginger (Zingiber officinale), has been reported to improve ulcerative colitis. The present study aims investigate therapeutic efficacy two analogous forms 6-gingerol, 8-gingerol, 10-gingerol, on Colitis was induced in rats through consumption 5% (w/v) dextran sulfate sodium drinking water for 7 consecutive days. 10-gingerol were then...
Deep venous thrombosis (DVT) of lower extremities is a common thrombotic disease, occurring either in isolation or as complication other diseases procedures. MiR-21 one important microRNAs which play critical role various cellular function. This study aim to determine the effect miR-21 on endothelial progenitor cells (EPCs) and its predicting prognosis DVT. EPCs was isolated from DVT models control subjects. expression confirmed by RT-PCR. Potential target mRNA predicted bioinformatics...
Although temozolomide (TMZ) is the most effective chemotherapy agent for glioma, resistance has limited its clinical use. Fluoxetine (FLT), which widely used in cancer-related depression, exhibited potent anticancer properties different cancer cell types. The aim of this study was i) to evaluate antitumor mechanism FLT, and ii) further effects a combination FLT TMZ on glioma cells. Glioma lines were exposed and/or TMZ. Cell viability apoptosis examined by CCK-8 assay, flow cytometry...
Acute myeloid leukemia can develop as myoblasts infiltrate into organs and tissues anywhere other than the bone marrow, which called extramedullary infiltration (EMI), indicating a poor prognosis. Circular RNAs (circRNAs) are novel class of non-coding that feature covalently closed continuous loops, suggesting their potential micro RNA (miRNA) "sponges" participate in biological processes pathogenesis. However, investigations on circRNAs EMI were conducted rarely. In this study, overall...
Abstract MicroRNAs (MiRNAs, MiRs) represent a class of conserved small non‐coding RNAs that affect post‐transcriptional gene regulation and play vital role in angiogenesis, proliferation, apoptosis, migration invasion. They are essential for wide range physiological pathological processes, especially vascular diseases. However, data concerning miRNAs endothelial progenitor cells (EPCs) deep vein thrombosis (DVT) remain incomplete. We explored modulate angiogenesis EPCs thrombolysis, analysed...
Hepatocellular carcinoma (HCC) has a high incidence in China and exploring effective ways for early diagnosis is an important method to improve the prognosis of patients with HCC. Additional studies reported that. Some kinds microRNA (miRNA) plasma will change accordingly during HCC progress, this can be used diagnose HCC, especially miRNA-122, miRNA-21 miRNA-96. We were aiming at investigating values exosomal miRNAs patients.Blood samples from 50 hepatic cirrhosis healthy volunteers...
Neobavaisoflavone (NBIF), a natural active ingredient isolated from Psoralea, possesses anti-inflammatory, anti-cancer, and antioxidant properties; however, the anti-tumor mechanism of NBIF has not been thoroughly investigated, inhibitory effect pathway on liver cancer are still unknown. Our study aimed to explore effects hepatocellular carcinoma its potential mechanisms. First, we detected inhibition HCC cells by CCK8 assay then observed morphological changes under microscope. Besides,...
Long non-coding RNAs (lncRNAs) play an essential role in multitudinous physiological and pathological processes, including vascular disease.We previously showed that lncRNA GUSBP5-AS (enst00000511042) is upregulated endothelial progenitor cells (EPCs) of deep vein thrombosis (DVT) patients.Here, we investigate the mechanism EPCs DVT.Using DVT model, found significantly reduced thrombus size weight enhanced homing ability EPC to sites promote resolution recanalization thrombus.GUSBP5-AS...
We investigated the protective effects and mechanism of action metformin on high glucose-induced smooth muscle cell proliferation migration.Vascular cells (VSMCs) were subjected to a series concentrations (0-10 mM) metformin.CCK-8, wound healing, transwell assays performed.Correlations between concentration high-mobility group box 1 (HMGB1) miR-142-3p levels assessed.In addition, mimic siRNA used investigate VSMC migration in presence or absence metformin.In high-glucose condition, decreased...
Abstract Background Oncolytic virotherapy is an attractive drug platform of cancer gene therapy, but efficacy and specificity are important prerequisites for success such strategies. Previous studies determined that Apoptin a p53 independent, bcl-2 insensitive apoptotic protein with the ability to specifically induce apoptosis in tumor cells. Here, we generated conditional replication-competent adenovirus (CRCA), designated Ad-hTERT-E1a-Apoptin, investigated effectiveness CRCA therapy agent...
Although the generation of BCR-ABL is molecular hallmark chronic myeloid leukemia (CML), comprehensive mechanisms disease remain unclear yet. Growth arrest specific 2 (GAS2) regulates multiple cellular functions including cell cycle, apoptosis and calpain activities. In present study, we found GAS2 was up-regulated in CML cells CD34+ progenitor compared to their normal counterparts. We utilized RNAi expression dominant negative form (GAS2DN) target GAS2, which resulted activity enhancement...
Endothelial to mesenchymal transition (EndMT) is an important pathological change in many diseases. Semaphorin7A (Sema7A) has been reported regulate nerve and vessel homeostasis, but its role EndMT remains unclear. Here we investigate the effect of Sema7A on underlying mechanism. Sema7A-overexpressed human umbilical vein endothelial cells (Sema7A-HUVECs) were generated showed lower levels cell markers higher indicating occurrence EndMT. RNA-sequencing analysis a total 1168 upregulated genes...
Abstract Venous thromboembolism, which includes deep venous thrombosis (DVT) and pulmonary embolism, is the third most common vascular disease in world seriously threatens lives of patients. Currently, effect conventional treatments on DVT limited. Endothelial progenitor cells (EPCs) play an important role resolution recanalization DVT, but unfavorable microenvironment reduces EPC function. Non-coding RNAs, especially long non-coding RNAs microRNAs, a crucial improving biological function...