A5101575267- Advanced Fluorescence Microscopy Techniques
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Blood properties and coagulation
- Immune Cell Function and Interaction
- Photoacoustic and Ultrasonic Imaging
- Cancer Genomics and Diagnostics
- Platelet Disorders and Treatments
- Molecular Biology Techniques and Applications
- Cutaneous lymphoproliferative disorders research
- Digital Storytelling and Education
- Advanced Biosensing Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- Renal and related cancers
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Lymphoma Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Fungal Infections and Studies
- Participatory Visual Research Methods
- Advanced Electron Microscopy Techniques and Applications
- Gout, Hyperuricemia, Uric Acid
- Blood transfusion and management
- Leptospirosis research and findings
Yale University
2013-2024
Massey University
2021-2023
John Wiley & Sons (United States)
2021
University of Colorado Denver
2019-2020
Children's Hospital Colorado
2020
Josep Carreras Leukaemia Research Institute
2019
Spanish National Cancer Research Centre
2019
VA Connecticut Healthcare System
2011-2016
Yale New Haven Hospital
2014-2016
Regeneron (United States)
1998-2012
Localizing cell surface receptors to specific subcellular positions can be critical for their proper functioning, as most notably demonstrated at neuronal synapses. PDZ proteins apparently play roles in such protein localizations. Receptor tyrosine kinases have not been previously shown interact with vertebrates. We report that Eph and membrane-linked ligands all contain recognition motifs bind clustered by proteins. In addition, we find colocalize Thus, may localizing vertebrate receptor...
<h3>Background:</h3> Monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystal-induced interleukin 1 β (IL1β) release contributes to inflammation in subcutaneous air pouch peritoneal models of acute gout pseudogout. However, consequences IL1 inhibition have not been explored more clinically relevant arthritis. <h3>Objective:</h3> To develop a novel mouse model gouty ankle arthritis use it assess the effects genetic deletion receptor type (IL1R1) exogenous mIL1 Trap (a...
Background In patients with autosomal dominant polycystic kidney disease (ADPKD), most of whom have a mutation in PKD1 or PKD2 , abnormally large numbers macrophages accumulate around cysts and promote their growth. Research by us others has suggested that monocyte chemoattractant protein-1 (Mcp1) may be signal for macrophage-mediated cyst Methods To define the role Mcp1 promoting growth, we used mice inducible knockout Pkd1 alone (single knockout) both (double murine renal tubule. Levels...
Abstract Comprehensive preclinical studies of Myelodysplastic Syndromes (MDS) have been elusive due to limited ability MDS stem cells engraft current immunodeficient murine hosts. Here we report a patient-derived xenotransplantation model in cytokine-humanized “MISTRG” mice that provides efficient and faithful disease representation across all subtypes. MISTRG xenografts (PDX) reproduce patients’ dysplastic morphology with multi-lineage representation, including erythro- megakaryopoiesis....
Abstract Immune-deficient mice, reconstituted with human stem cells, have been used to analyze immune responses in vivo. Although they study xenografts, allografts, and pathogens, there not models of autoimmune disease which the mechanisms pathologic process can be analyzed. We found that “humanized” mice treated anti–CTLA-4 Ab (ipilimumab) develop characterized by hepatitis, adrenalitis, sialitis, anti-nuclear Abs, weight loss. Induction autoimmunity involved activation T cells cytokine...
Abstract A peripheral blood flow cytometric assay for Sézary syndrome (SS) or circulating mycosis fungoides (MF) cells must be able to reliably identify, characterize, and enumerate T‐cells with an immunophenotype that differs from non‐neoplastic T‐cells. Although it is also important distinguish SS MF other subtypes of T‐cell neoplasm, this usually requires information in addition the immunophenotype, such as clinical morphologic features. This article outlines approach recommended by...
Morphologic profiling of the erythrocyte population is a widely used and clinically valuable diagnostic modality, but one that relies on slow manual process associated with significant labor cost limited reproducibility. Automated erythrocytes from digital images by capable machine learning approaches would augment throughput value morphologic analysis. To this end, we sought to evaluate performance leading implementation strategies for convolutional neural networks (CNNs) when applied...
The rapid acquisition of biological data and development computationally intensive analyses has led to a need for novel approaches software deployment. In particular, the complexity common analytic tools genomics makes them difficult deploy decreases reproducibility computational experiments.Recent technologies that allow application virtualization, such as Docker, developers bioinformaticians isolate these applications secure, scalable platforms have potential dramatically increase...
This study characterizes the percentage of pediatric patients undergoing liver transplant who were up to date for their age on immunizations at time and risk factors underimmunization.
Neuromedin U (NMU) has recently been reported to have a role in nociception and inflammation. To clarify the function of two known NMU receptors, receptor 1 (NMUR1) 2 (NMUR2), during inflammation vivo, we generated mice which genes for each were independently deleted. Compared wild type littermates, deficient NMUR2 showed reduced thermal nociceptive response hot plate, but not tail flick, test. In addition, mutant behavioral marked reduction hyperalgesia following capsaicin injection....
Context.—Despite continuing advances in tissue processing automation, traditional embedding, cutting, and staining methods limit our ability for rapid, comprehensive visual examination. These limitations are particularly relevant to biopsies which immediate therapeutic decisions most necessary, faster feedback the patient is desired, preservation of ancillary studies important. The recent development improved clearing techniques has made it possible consider use multiphoton microscopy (MPM)...
The risks of transfusion remain significant. Familiarity with the incidence, etiology, management, and prevention commonly encountered reactions is integral to practice medicine. This review intends serve as a practical guide reflecting current understanding adverse events, clinical features helpful for diagnosis, recommended management strategies typical scenarios. Severe mild are covered, focus on distinguishing challenging dilemmas. Topics include pulmonary complications transfusion,...
ABSTRACT Oropharyngeal and esophageal candidiasis (OPEC) is a frequent opportunistic mycosis in immunocompromised patients. Azole-resistant OPEC refractory form of this infection occurring particularly human immunodeficiency virus (HIV)-infected The procedures developed by the Antifungal Subcommittee National Committee for Clinical Laboratory Standards (NCCLS) are an important advance standardization vitro antifungal susceptibility methodology. In order to further understand relationship...
Background and Aims The etiology of biliary atresia (BA) is not known likely multifactorial, including a genetic predisposition, viral or environmental trigger, an aberrant autoimmune response targeting cholangiocytes, unique susceptibilities the neonatal bile ducts to injury. Damaged cholangiocytes may express neo self‐antigens elicit autoreactive T‐cell‐mediated inflammation B‐cell production autoantibodies. aim this study was discover autoantibodies in BA that correlated with outcomes....
Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon rate as compared to state-of-the-art tubes. demonstrate this capability dramatically improve both rates...
Multiphoton microscopy of cleared tissue has previously been demonstrated to generate large three-dimensional (3D) volumetric image data on entire intact mouse organs using intrinsic fluorescence. This technique holds great promise for performing 3D virtual biopsies, providing unique information morphology, and guidance subsequent traditional slicing staining. Here, we demonstrate the use fluorescence lifetime imaging in achieving molecular contrast that can reveal morphologically distinct...