A5009775875- Nitric Oxide and Endothelin Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Angiogenesis and VEGF in Cancer
- Atherosclerosis and Cardiovascular Diseases
- Renin-Angiotensin System Studies
- Hormonal Regulation and Hypertension
- Axon Guidance and Neuronal Signaling
- Cancer-related molecular mechanisms research
- Apelin-related biomedical research
- RNA modifications and cancer
- Cardiovascular Function and Risk Factors
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Cardiac Structural Anomalies and Repair
- Lipid metabolism and disorders
- Cardiac Fibrosis and Remodeling
- Mechanical Circulatory Support Devices
- Signaling Pathways in Disease
- Caveolin-1 and cellular processes
- Acute Kidney Injury Research
- Platelet Disorders and Treatments
- Bacteriophages and microbial interactions
- Hippo pathway signaling and YAP/TAZ
- Lipoproteins and Cardiovascular Health
- Erythropoietin and Anemia Treatment
University Hospital Carl Gustav Carus
2004-2022
TU Dresden
2007-2022
Friedrich Schiller University Jena
2021
Fraunhofer Institute for Cell Therapy and Immunology
2008
Heidelberg University
2005-2008
University Hospital Heidelberg
2008
Martin Luther University Halle-Wittenberg
2000-2006
Leipzig Heart Institute
2006
Swiss National Science Foundation
2002
Deutsche Forschungsgemeinschaft
2002
Objectives Vasoconstrictor prostanoids have been implicated in abnormal vasomotion atherosclerosis and hypertension. Method Using lean diet-induced obese mice, we investigated whether obesity affects vascular function or expression of genes involved prostanoid action. Results In C57BL/6J at high concentrations acetylcholine caused endothelium-dependent contractions the carotid artery but not aorta. Endothelium-dependent to were blocked by non-selective cyclooxygenase (COX) inhibitors...
Abstract —In the C57BL/6J mice model, we investigated whether obesity affects function or expression of components tissue renin-angiotensin system and endothelin (ET)-1 contributes to these changes. ACE activity (nmol · L His-Leu mg protein −1 ) was measured in lung, kidney, liver control (receiving standard chow) obese animals treated for 30 weeks with a high-fat, low cholesterol diet alone combination LU135252 , an orally active ET A receptor antagonist. mRNA effects on purified human were...
Abstract Endothelial cells are constantly exposed to high or low shear stress in arteries and veins by the flowing blood. Angiopoietin‐2 (Ang‐2) is acting as a critical regulator of vessel maturation endothelial cell quiescence. In this study, flow‐dependent regulation Ang‐2 was analyzed vitro vivo. mRNA, protein expression release upregulated 24 h (1 dyne/cm 2 ), but downregulated flow (30 ) human cells. Increased NO synthase formation not affecting flow. Low increased VEGF‐A expression....
Statins and angiotensin type 1 (AT1) receptor blockers reduce cardiovascular mortality morbidity. In the Endothelial Protection, AT1 blockade Cholesterol-Dependent Oxidative Stress (EPAS) trial, impact of independent or combined statin blocker therapy on endothelial expression anti-atherosclerotic proatherosclerotic genes function in arteries patients with coronary artery disease were tested.Sixty stable undergoing elective bypass grafting (CABG) surgery randomized 4 weeks before to: (A)...
The endothelium controls blood flow and pressure by releasing several vasoactive factors, among them the vasodilator nitric oxide (NO) potent vasoconstrictor endothelin-1 (ET-1). Although increased NO levels have been found in excessive erythrocytosis, little is known concerning ET-1 expression this condition. Thus, we examined endothelin system transgenic mice that due to constitutive overexpression of erythropoietin (Epo) reached hematocrit approximately 80%. Surprisingly, despite...
Objective— The EphB ligand ephrinB2 has been identified as a critical determinant of arterial endothelial differentiation and positive regulator invading cells during angiogenesis. This study was aimed at identifying determinants cell expression. Methods Results— Arteriovenous asymmetrical expression in vivo is lost on transfer into culture with aortic becoming partially ephrinB2-negative saphenous vein ephrinB2-positive. Contact smooth muscle angiogenic stimulation by vascular growth factor...
Endothelial cells in vivo are constantly exposed to mechanical forces such as cyclic strain. In endothelial cells, Nox4-containing NAD(P)H oxidase complexes have been identified major sources of superoxide anion (·O2−) formation. this study, we analyzed the effect strain on ROS formation by electron paramagnetic resonance spectroscopy, cytochrome c assay, and dihydroethidium fluorescence, NO Griess reaction gene expression RT-PCR Western blot. Primary cultures human umbilical vein were 2–18%...
Ventricular assist devices (VAD) are implanted in patients with end-stage heart failure for bridging the time until transplantation, resulting hemodynamic unloading of failing heart, improved cardiac contractile and mitochondrial function, reversal hypertrophy. It is unknown whether VAD may affect endothelin (ET) system, which has been proposed as one putative pathomechanisms failure.With use standard-calibrated, competitive reverse-transcription-polymerase chain reaction mRNA expression...
The EphB receptors and their ephrinB ligands are involved in vascular assembly differentiation. In this study, the authors analyzed regulation of ephrinB2 EphB4 response to laminar shear stress human endothelial cells. order simulate different flow conditions vitro, cells were exposed (1 50 dyn/cm2 for up 24 h) a cone-and-plate viscometer. EphrinB2 mRNA expression is down-regulated by arterial, but not venous, dose-dependent manner primary cultures umbilical vein (HUVECs) (maximum at 30...
Diabetes mellitus is an important risk factor for cardiovascular diseases. Clinical evidence supports a link between hyperglycemia, endothelial dysfunction, and vascular disorders. However, the precise molecular mechanisms causing dysfunction in diabetic patients remain unclear. An interesting novel mediator could be chicken ovalbumin upstream promoter-transcription II (COUP-TFII), which plays essential role glucose metabolism. COUP-TFII known to expressed venous cells. In this study, we...
Our objective was to study the kinetics of circulating endothelial cells (EC) and precursor (EPC) in hematological patients during chemotherapy autologous stem cell transplantion (ASCT). Eighteen newly diagnosed 17 undergoing ASCT were studied compared healthy controls. ECs evaluated as CD146+CD31+Lin- cells, while EPCs CD34+CD133+Lin-, or CD34+VEGFR2+Lin- CFU-En colony forming units. Numbers these before after treatment, and, treated with ASCT, mobilization hematopoietic progenitors. Both...
Obesity is a well-known risk factor of atherosclerosis and heart failure. In the human heart, local endothelin system containing prepro-endothelin-1, endothelin-converting enzyme-1, receptors A B has been described. The activated in failure; however, impact obesity on cardiac unknown. this study, 18-week-old male C57BL/6 mice fed either control diet or high-fat for 10 weeks were analyzed. High-fat significantly increased body weight animals augmented low-density lipoprotein, high-density...
Summary: Using the orally active endothelin-A- (ETA) receptor antagonist LU135252, we determined whether endothelin-1 (ET-1) and/or dietary fat may be involved in angiotensin-converting enzyme (ACE) regulation vivo. In C57BL6/J mice, renal and pulmonary tissue ACE activity (nmol/1 His-Leu/mg protein) was measured mRNA expression, ET-1 protein content nitrite/nitrate level were kidney. Western-type diet increased by 70% (55 ± 4 vs 33 3 nmol/1 protein, p < 0.05) levels (267 19 pg/g 190 18,...
Many recent studies highlight the fundamental importance of viruses. Besides their important role as human and animal pathogens, beneficial, commensal or harmful functions are poorly understood. By developing applying tailored bioinformatical tools in virological models, Marie Skłodowska-Curie Initiative International Training Network VIROINF will provide a better understanding viruses interaction with hosts. This open door to validate methods improving viral growth, morphogenesis...
In vivo, endothelial cells are constantly exposed to shear stress by flowing blood. Short-term exposure of has been shown induce endothelin-1 release. It is currently unknown, however, whether this stress-dependent release affects the expression and activity transcription factors. study, primary cultures human from umbilical vein were laminar in a cone-and-plate viscometer. Laminar for 30 min induced 2-fold increase mRNA c-jun , but not c-fos, cells. Blockade endothelin receptor subtype B...
A key step in endothelin-1 (ET-1) synthesis is the proteolytic cleavage of big ET-1 by endothelin-converting enzyme-1 (ECE-1). Four alternatively spliced isoforms, ECE-1a to ECE-1d, have been discovered; however, regulation expression specific ECE-1 isoforms not well understood. Therefore, we stimulated primary human umbilical vein endothelial cells (HUVECs) with angiotensin II (Ang II). Furthermore, was determined internal mammary arteries patients undergoing coronary artery bypass grafting...