A5100683296- Nuclear Receptors and Signaling
- Cell death mechanisms and regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Myeloid Leukemia Research
- Osteoarthritis Treatment and Mechanisms
- Medicinal Plants and Neuroprotection
- Histone Deacetylase Inhibitors Research
- Educational Research and Pedagogy
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Beetle Biology and Toxicology Studies
- Innovative Educational Techniques
- Insect-Plant Interactions and Control
- Genomics, phytochemicals, and oxidative stress
- S100 Proteins and Annexins
- Spinal Hematomas and Complications
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Ion Channels and Receptors
- Insect and Pesticide Research
- Protease and Inhibitor Mechanisms
- Adrenal Hormones and Disorders
- Cancer therapeutics and mechanisms
- Advanced biosensing and bioanalysis techniques
- Diverse Approaches in Healthcare and Education Studies
Suqian University
2024
University of Freiburg
2024
Shandong Agricultural University
2024
Zhujiang Hospital
2023
Southern Medical University
2023
Nanjing Medical University
2023
Suzhou Municipal Hospital
2023
Southwest University
2017-2021
Fourth People’s Hospital of Jinan
2021
Shandong First Medical University
2021
Autophagy plays a pro-survival role in the tamoxifen-resistant breast cancer cells. Herein we found that autophagy was concomitantly induced MCF-7 (MCF-7TR5) cells through dissociation of Bcl-2 from Beclin 1 and subsequent enhancement interaction among ATG14-Beclin1-PI3KC3 complex. Moreover, higher level DNA damage observed MCF-7TR5 with decreased BRCA1 RAD51 increased Ku80 level. Interestingly, Nur77 selectively degraded by autophagy, which causes release Nur77-Ku80 complex, resulting...
Purpose: This study aims to observe the effects of notopterol on apoptosis and differentiation HL-60 cells explore underlying molecular mechanisms. Methods: Cell viability was assessed using sulforhodamine B assay. proliferation determined by trypan blue dye exclusion test. Colony-forming units were assayed in methylcellulose. Apoptosis assays carried out annexin V-fluorescein isothiocyanate(FITC)/propidium iodide (PI) double staining, Hoechst 33342 mitochondrial membrane potential, Western...
Ginsenoside 20(S)-Rh2 has been shown to induce apoptosis and differentiation of acute myeloid leukemia (AML) cells. However, the underlying molecular mechanisms are not fully understood. In our study, induced expression orphan nuclear receptor Nur77 death proteins Fas, FasL, DR5, TRAIL, as well cleavage caspase 8 3 in HL-60 Importantly, shNur77 attenuated 20(S)-Rh2-induced Fas DR5 expression. Meanwhile, promoted translocation from nucleus mitochondria enhanced interaction between Bcl-2,...
Isobavachalcone (IBC) has been shown to induce apoptosis and differentiation of acute myeloid leukemia (AML) cells. However, the underlying molecular mechanisms are not fully understood. Herein, IBC exhibited significant inhibition on cell viability, proliferation, colony formation ability AML Moreover, induced mitochondrial evidenced by reduced membrane potential, increased Bax level, decreased Bcl‐2, Bcl‐xL, Mcl‐1 levels, elevated cytochrome c level in cytosol cleavage caspase‐9,...
// Hui Ma 1, * , Li Guojun Dou 1 Chengqiang Wang Juan He Mingxia Wu and Hongyi Qi College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China These authors have contributed equally to this work Correspondence to: Qi, email: hongyiqi@swu.edu.cn Keywords: Z-ligustilide, tamoxifen, ERα negative breast cancer, MTA1, histone modification Received: January 13, 2017 Accepted: March 02, Published: 22, ABSTRACT Emerging evidence indicates epigenetic represses estrogen receptor α...
β-asarone, the main component in volatile oil of Acori tatarinowii Rhizoma, has been found to possess antitumor activity. However, its effect and mechanisms against tumor invasion epithelial-mesenchymal transition (EMT) are still unclear. In this study, no or less cytotoxicity was caused by β-asarone within 0-120 μM human glioma U251 cells for 48 h. (30 60 μM) inhibited migration wound healing assay, suppressed Boyden chamber adhesion onto Matrigel. Moreover, EMT with up-regulation...
HnRNP A2/B1 has been found to be an oncogenic protein strongly related the growth of human glioma cells. Herein, β-asarone, main component in volatile oil Acori tatarinowii Rhizoma, inhibited cell viability, proliferation, and colony formation ability U251 Moreover, β-asarone induced apoptosis cycle arrest at G1 phase. Notably, suppressed expression hnRNP hnRNPA2/B1 overexpression remarkably reversed β-asarone-mediated arrest. Importantly, promoted alternative splicing Bcl-x by enhancing...
Plants have evolved a variety of phytochemicals to defense insect feeding, whereas insects also diverse detoxification enzymes, which are adaptively induced as prosurvival mechanism. Herein, Z-ligustilide in Ligusticum chuanxiong Hort. was found exhibit similar trend the accumulation from December May occurrence Spodoptera litura (Fabricius) larvae. Importantly, S. larvae feeding enhanced level stem and leaf (p < 0.01). Moreover, ranging 1 5 mg·g-1 exhibited remarkable larvicidal activity,...
Acute myeloid leukemia (AML) is a hematopoietic malignancy characterized by uncontrolled proliferation and accumulation of myeloblasts in the bone marrow (BM), blood, other organs. The nuclear receptors Nur77 common feature leukemic blasts has emerged as key therapeutic target for AML. Cantharidin (CTD), main medicinal component Mylabris (blister beetle), exerts an anticancer effect multiple types cancer cells. This study aims to characterize anti-AML activity CTD vitro vivo explore...
Diabetes mellitus- (DM-) associated hyperglycemia promotes apoptosis of disc nucleus pulposus (NP) cells, which is a contributor to intervertebral degeneration (IDD). Melatonin able protect against cell apoptosis. However, its effects on NP in high-glucose culture remain unclear. The purpose the present study was investigate and molecular mechanism melatonin culture. cells were cultured baseline medium supplemented with concentration (0.2 M) for 3 days. control only medium. Additionally,...
Osteoarthritis (OA) is a chronic joint disease with increasing prevalence. Chondrocytes (CHs) are highly differentiated end-stage cells secretory phenotype that keeps the extracellular matrix (ECM) balanced and cartilage environment stable. dedifferentiation causes breakdown, accounting for one of key pathogenesis osteoarthritis. Recently, activation transient receptor potential ankyrin 1 (TRPA1) was claimed to be risk factor in osteoarthritis by causing inflammation degradation. However,...
Osteoarthritis (OA) is a chronic disease due to the deterioration of cartilage structure and function, involving progressive degradation extracellular matrix. Cathepsins, lysosomal cysteine proteases, play pivotal roles in various biological pathological processes, particularly protein degradation. Excess cathepsins levels are reported contribute development OA. However, causal relationship between cathepsin family knee hip OA remains uncertain. Therefore, this study utilized bidirectional...
BACKGROUND: Respiratory epithelium expressing angiotensin-converting enzyme 2 (ACE2) is the entry for novel coronavirus (SARS-CoV-2), pathogen of COVID-19 pneumonia outbreak, although a few recent studies have found different ACE2 expression in lung tissue smokers. The effect smoking on and still not clear. So, we did this research to determine pattern its relationship with risk severity COVID-19. METHODS: clinical data patients non-smoking were analyzed, respiratory digestive mucosa...
Objectives: This study aimed to identify the pivotal roles of genetic signatures immune cells in frozen shoulder (FS) for diagnosis and therapy, verify consequence widely used glucocorticoids (GCs) FS treatment.Methods: RNA sequencing (RNA-seq) was conducted on normal adhesive capsular tissues cell subtypes expression differential genes involved FS. The Xcell tool utilized resolve heterogeneity data quantify types. A two-sample Mendelian randomization (MR) analysis then performed using 731...
The innovation competence of K-12 education teachers undoubtedly plays a crucial role in fostering the abilities their students. mathematics equips students with critical thinking and problem-solving skills essential for future studies colleges universities, helping them grasp complex techniques to address challenges everyday life careers. Therefore, it is great significance study strategies improving college majoring Mathematics Education, as they will likely become directly after...
Spinal cord injury (SCI) has emerged as a prevalent condition with limited effective treatment options. The neuroprotective role of pramipexole (PPX) in inhibiting nerve cell apoptosis central nervous system injuries is well established. Therefore, we investigated the effects PPX SCI. Adult Sprague-Dawley rats were divided into four groups (sham, SCI, PPX-0.25, and PPX-2.0 groups) according to therapy (n = 24). Then, SCI was induced using modified Allen method, intravenously administered...
Objective This study tends to establish a data processing method for the learning type of medical statistics course through test item analysis.Methods An investigation was carried out on 92 students graded 2008 from department clinical medicine.It compares and analyzes variation exam scores.Results The significance level multi-coefficient confirmed as α1 =0.0167.Comparing with items problem analysis,the variations single-choice,filling blank,and explanation conception were significantly...