A5043962728- Hair Growth and Disorders
- Wnt/β-catenin signaling in development and cancer
- CAR-T cell therapy research
- Dermatology and Skin Diseases
- Nerve injury and regeneration
- Skin and Cellular Biology Research
- Psoriasis: Treatment and Pathogenesis
- Autoimmune Bullous Skin Diseases
- Developmental Biology and Gene Regulation
- Exercise and Physiological Responses
- Nanofabrication and Lithography Techniques
- Axon Guidance and Neuronal Signaling
- Drug-Induced Adverse Reactions
- Chronic Myeloid Leukemia Treatments
- Genomics and Chromatin Dynamics
- Silk-based biomaterials and applications
- Biotin and Related Studies
- Transgenic Plants and Applications
- Electrospun Nanofibers in Biomedical Applications
- Tendon Structure and Treatment
- Surgical Sutures and Adhesives
- Virus-based gene therapy research
- Chronic Lymphocytic Leukemia Research
- Reproductive System and Pregnancy
- Advancements in Semiconductor Devices and Circuit Design
University of Pennsylvania
2014-2025
California University of Pennsylvania
2014
Ideally, therapy for autoimmune diseases should eliminate pathogenic cells while sparing protective immunity, but feasible strategies such an approach have been elusive. Here, we show that in the antibody-mediated disease pemphigus vulgaris (PV), autoantigen-based chimeric immunoreceptors can direct T to kill autoreactive B lymphocytes through specificity of cell receptor (BCR). We engineered human express a autoantibody (CAAR), consisting PV autoantigen, desmoglein (Dsg) 3, fused CD137-CD3ζ...
Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed mammals. We discovered mouse that during wound healing, adipocytes regenerate from myofibroblasts, a type thought be differentiated nonadipogenic. Myofibroblast required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling then activation adipocyte transcription factors expressed development. Overexpression BMP antagonist Noggin...
A chimeric antigen receptor redirects T cells to treat glioblastoma.
Sweat out the fat with TSLP! Thymic stromal lymphopoietin (TSLP) is a cytokine that can promote immune responses characterize allergic diseases. Choa et al . found mice engineered to produce elevated TSLP displayed selective white adipose tissue loss protected them from obesity, insulin resistance, and steatohepatitis (see Perspective by Schneider). Protection was not mediated eosinophils, regulatory T cells, or innate lymphoid cells. Rather, TLSP induced migration of conventional cells...
Most wounds form scars without hair follicles. However, in the wound-induced neogenesis (WIHN) model of skin regeneration, regenerate follicles if tissue rigidity is optimal. Although WIHN depends on Wnt signaling, whether performs a mechanoregulatory role that contributes to regeneration remains uncharacterized. Here, we demonstrate signaling affects mechanosensitivity at both cellular and levels drive WIHN. Atomic force microscopy revealed an attenuated substrate response epidermal but not...
Abstract The hair follicle is considered to be a model system for studying organogenesis. In our initial study using mouse cells (Zheng et al., 2005) we found that new formation always starts from an epithelial platform: the epidermal aggregate, aggregates encyst, and periphery of cysts, centrifugally, buds, pegs, follicles form. this report, extend four distantly related mammals: opossum, rat, dog human. We find in these species, plus mouse, most trichogenic are earliest stages development...
Enhancers are context-specific regulators of expression that drive biological complexity and variation through the redeployment conserved genes. An example this is enhancer-mediated control Engrailed 1 (EN1), a pleiotropic gene whose required for formation mammalian eccrine sweat glands. We previously identified En1 candidate enhancer (ECE) 18 cis-regulatory element has been highly repeatedly derived on human lineage to potentiate ectodermal EN1 induce our species' uniquely high gland...
Janus kinase (JAK) inhibitors are approved for many dermatologic disorders, but their use is limited by systemic toxicities including serious cardiovascular events and malignancy. To overcome these limitations, injectable hydrogels engineered the local sustained delivery of baricitinib, a representative JAK inhibitor. Hydrogels formed via disulfide crosslinking thiolated hyaluronic acid macromers. Dynamic thioimidate bonds introduced between nitrile-containing baricitinib drug tethering,...
Abstract Within the past 30 years, obesity and associated disorders such as Type II diabetes are rapidly becoming world-wide epidemics. diabetes, caused by loss of insulin sensitivity, accounts for $327 billion annually in U.S. Recent studies have shown that immune system plays a critical role regulating metabolic function adipose tissue. Importantly, is disease chronic low-grade inflammation, T regulatory cells (Tregs) can help maintain sensitivity suppressing inflammation monocytes, CD8 T,...
Meeting abstracts Chimeric antigen receptors are synthetic molecules designed to re-direct T cells specific surface antigens; CAR-modified can mediate long-term durable remissions in B cell malignancies, but expanding this platform solid tumors requires the discovery of novel
Abstract Obesity is a serious public health concern. Emerging studies indicate that the immune system can regulate weight and energy homeostasis. Here we show cytokine Thymic Stromal Lymphopoietin (TSLP) stimulates T cells to induce selective white adipose loss, which protects against obesity, improves glucose metabolism, mitigates nonalcoholic steatohepatitis. Surprisingly, loss was not caused by alterations in food intake, absorption, or expenditure. Rather, it excessive of lipids through...
OBJECTIVES/GOALS: Current clinical practice recommends occlusive dressings (e.g., film and hydrocolloid) for wounds with variable regenerative capacities. However, evidence suggests that occlusion may hinder regeneration. Our objective was to test the impact of on regeneration using animal models. METHODS/STUDY POPULATION: The murine wound-induced hair neogenesis (WIHN) is a well-established model characterized by de novo follicle (HF) formation in center large full-thickness wounds....