A5081858883- Cerebrovascular and genetic disorders
- Nitric Oxide and Endothelin Effects
- Erythrocyte Function and Pathophysiology
- Neuroscience of respiration and sleep
- Ion channel regulation and function
- Blood properties and coagulation
- Ion Channels and Receptors
- High Altitude and Hypoxia
- Magnesium in Health and Disease
- Congenital limb and hand anomalies
- Liver physiology and pathology
- Cellular Mechanics and Interactions
- Orthopedic Surgery and Rehabilitation
- Physiological and biochemical adaptations
- Hemoglobin structure and function
- Virus-based gene therapy research
- RNA Research and Splicing
- RNA modifications and cancer
- Chemical and Physical Studies
- Neurogenetic and Muscular Disorders Research
- Liver Diseases and Immunity
- Liver Disease Diagnosis and Treatment
- Renin-Angiotensin System Studies
- Spaceflight effects on biology
- Vascular Tumors and Angiosarcomas
Victor Chang Cardiac Research Institute
2019-2024
Peking University
2005-2023
Beijing Jishuitan Hospital
2005-2023
UNSW Sydney
2019-2023
Sun Yat-sen University
2019-2023
Institute of Infection and Immunity
2022
Whitehead Institute for Biomedical Research
2018-2022
Shanghai Medical College of Fudan University
2022
Shanghai First People's Hospital
2022
Nanjing Medical University
2022
Mitochondrial serine transporter identified One-carbon (1C) metabolism is a universal metabolic process that required for purine synthesis and supports the high levels of proliferation in cancer cells. The transport into mitochondria supplies most 1C units needed biosynthesis. Kory et al. used genetic screen to identify long-sought-after mitochondrial transporter. Elucidating key step important our understanding has potential implications treatment. Science , this issue p. eaat9528
Pressure overload-induced cardiac hypertrophy is a maladaptive response with poor outcomes and limited treatment options. The transient receptor potential melastatin 4 (TRPM4) ion channel key to activation of Ca
The aims of this study were to assess the role nitric oxide (NO) and contribution different NO synthase (NOS) isoforms in skeletal muscle contractile dysfunction septic shock. Four groups conscious rats examined. Group 1 served as control; group 2, 3, 4 injected with Escherichia coli endotoxin [lipopolysaccharide (LPS), 20 mg/kg i.p.] killed after 6, 12, 24 h, respectively. Protein expression was assessed by immunoblotting immunostaining. LPS injection elicited a transient inducible NOS...
Mechanosensitive PIEZO1 channels have emerged as key transducers of mechanical forces in the cardiovascular system. In cardiomyocytes, we previously showed that decodes cues driving pressure-overload induced hypertrophy. However, conflicting reports exist on influence baseline cardiac function. Here show conditional deletion Piezo1 from cardiomyocytes adult mice results premature mortality. The hearts these exhibited signs accelerated aging, including elevated markers senescence associated...
Pathological left ventricular hypertrophy (LVH) occurs in response to pressure overload and remains the single most important clinical predictor of cardiac mortality. The molecular pathways induction LVH are potential targets for therapeutic intervention. Current treatments aim remove stimulus LVH, but do not completely reverse adverse remodelling. Although numerous signalling steps have been identified, initial step by which mechanical stretch associated with is converted into a chemical...
The transient receptor potential melastatin 4 (TRPM4) channel contributes extensively to cardiac electrical activity, especially cardiomyocyte action formation. Mechanical stretch can induce changes in heart rate and rhythm, the mechanosensitive Piezo1 is expressed many cell types within myocardium. Our previous study showed that TRPM4 are closely co-localized t-tubules of ventricular cardiomyocytes contribute Ca
Abstract The importance of mechanosensory transduction pathways in cellular signalling has prominently come to focus the last decade with discovery Piezo ion channel family. Mechanosignaling involving Piezo1 channels function heart and cardiovascular system only recently been identified have implications for physiology pathophysiology, particular failure (i.e., hypertrophy or dilative cardiomyopathy). These results emphasized need higher throughput methods study single-cell mechanobiology...
Abstract Mechanosensitive channels are integral membrane proteins that sense mechanical stimuli. Like most plasma ion channel they must pass through biosynthetic quality control in the endoplasmic reticulum results them reaching their destination at membrane. Here we show N-linked glycosylation of two highly conserved asparagine residues ‘cap’ region mechanosensitive Piezo1 necessary for mature protein to reach Both mutation these asparagines (N2294Q/N2331Q) and treatment with an enzyme...
The aim of this study was to assess the influence prolonged in vivo hypoxia on cerebellar nitric oxide (NO) production. Conscious rats were exposed 10% O2 (balanced N2) for 12 or 48 hr (arterial PO2 between 35 and 39 mmHg). animals then killed, cerebella quickly frozen. NO production measured vitro by monitoring conversion L-[3H]arginine L-[3H]citrulline. Protein mRNA expressions neuronal (nNOS) endothelial (ecNOS) isoforms synthases assessed using immunoblotting semiquantitative reverse...
Nitric oxide synthases (NOS) convert L-arginine to nitric in the presence of tetrahydrobiopterin (BH4). Two constitutive isoforms NOS exist normal skeletal muscle fibers, however, existence a third, inducible isoform (iNOS), has never been detected these fibers vivo. Therefore, we assessed influence vivo endotoxemia on expression and GTP cyclohydrolase I, rate limiting enzyme BH4 synthesis. groups rats were infused i.p. either with E. coli endotoxin (20 mg/kg, LPS group) or saline (saline...
Autophagy is an essential process in eukaryotic cells which autophagosomes form to deliver cellular organelles and long-lived proteins lysosomes for degradation. Many studies have recently identified the regulatory mechanisms involved interaction between viral infection autophagy. LC3 turnover endoplasmic reticulum (ER) stress pathway were investigated using western blot analysis. The formation degradation of detected immunofluorescence staining. was activated by porcine reproductive...
We retrospectively reviewed 12 minimally displaced fractures of the scaphoid waist in patients who developed delayed or nonunions with without conservative treatment. Mean time between injury and surgery was 6 months (range 3–12). The were stabilized double screws, which percutaneously inserted robot assistance, bone grafting. All united at a mean 8 weeks 6–10) after surgery. followed-up 1 year. recovered good wrist function. No major postoperative complications reported, returned to their...
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Increased nitric oxide (NO) synthesis by the inducible synthase (iNOS) has been shown to contribute development of acute lung injury and delayed hypotension in animals injected with bacterial lipopolysaccharides (LPS). Recent evidence indicates that endothelin-1 (ET-1) is also elevated septic humans animals. To assess contribution ETs LPS-induced pulmonary NO production iNOS expression, we used P1/fl, a 22 amino acid peptide, selectively antagonize endothelin-A receptors. Anesthetized,...
Nitric oxide (NO) is synthesized in normal muscle fibers by the neuronal (nNOS) and endothelial (ecNOS) isoforms of nitric synthase (NOS). NO contributes to regulation several processes such as excitation-contraction coupling mitochondrial respiration. We assessed this study whether production regulated response an acute increase activation. Three groups anesthetized, tracheostomized, spontaneously breathing rats were examined after experimental period 3 h. Group 1 served a control (no...
Abstract Sensing mechanical stimuli is crucial for the function of internal and external tissues, such as skin muscles. Much our understanding mechanosensory physiology relies on rodent studies, which may not directly translate to humans. To address knowledge gap in human mechanosensation, we developed distinct populations neuronal subtypes from pluripotent stem cells (hPSC). By inducing co-expression NGN2/RUNX3 or NGN2/SHOX2 hPSC-derived migrating neural crest directed their specification...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES