A5027416933- Respiratory viral infections research
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Bacterial Infections and Vaccines
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Malaria Research and Control
- COVID-19 Clinical Research Studies
- Infectious Diseases and Tuberculosis
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Tuberculosis Research and Epidemiology
- Infective Endocarditis Diagnosis and Management
- COVID-19 epidemiological studies
- Plant responses to water stress
- T-cell and Retrovirus Studies
- Rice Cultivation and Yield Improvement
- Infectious Encephalopathies and Encephalitis
- Vector-borne infectious diseases
- Genomics and Phylogenetic Studies
- Biosensors and Analytical Detection
Oxford University Clinical Research Unit
2010-2024
Hospital for Tropical Diseases
2010-2020
Seonam University
2020
Seoul Metropolitan Government
2020
Seoul National University
2020
Boramae Medical Center
2020
Center for Disease Control
2020
Children's Hospital
2018
University of Oxford
2012-2018
New York Medical College
2017
Abstract Background Little is known about the natural history of asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Methods We conducted a prospective study at quarantine center for disease 2019 in Ho Chi Minh City, Vietnam. enrolled quarantined people with reverse-transcription polymerase chain reaction (RT-PCR)–confirmed SARS-CoV-2 infection, collecting clinical data, travel and contact history, saliva enrollment daily nasopharyngeal/throat swabs (NTSs)...
We describe the magnitude and kinetics of plasma viremia nonstructural protein 1 (sNS1) levels in sequential samples from 167 children with acute dengue, enrolled early a community study Vietnam. All recovered fully, only 5 required hospitalization. Among those dengue virus type (DENV-1), was significantly greater primary (49) than secondary (44) infections took longer to resolve. In DENV-2 3 infections, lower among DENV-1 infections. Concentrations sNS1 were higher for after adjusting...
A better description of the extent and structure genetic diversity in dengue virus (DENV) endemic settings is central to its eventual control. To this end we determined complete coding region sequence 187 DENV-2 genomes 68 E genes from viruses sampled Vietnamese patients between 1995 2009. Strikingly, an episode genotype replacement was observed, with Asian 1 lineage entirely displacing previously dominant Asian/American viruses. This event also seems have occurred within Thailand Cambodia,...
BackgroundXpert MTB/RIF Ultra (Xpert Ultra) might have higher sensitivity than its predecessor, Xpert (Xpert), but role in tuberculous meningitis diagnosis is uncertain. We aimed to compare with for the of HIV-uninfected and HIV-infected adults.MethodsIn this prospective, randomised, diagnostic accuracy study, adults (≥16 years) suspected from a single centre Vietnam were randomly assigned cerebrospinal fluid testing by either or at baseline and, if treated meningitis, after 3–4 weeks...
We prospectively studied 3,791 children hospitalized during 2011 a large outbreak of enterovirus 71-associated hand, foot, and mouth disease in Vietnam. Formal assessment public health interventions, use intravenous immunoglobulin other therapies, factors predisposing for progression is needed to improve clinical management.
Abstract Background One hundred days after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Vietnam on 23 January, 270 cases were confirmed, with no deaths. We describe the control measures used by government and their relationship imported domestically acquired case numbers, aim of identifying associated successful SARS-CoV-2 control. Methods Clinical demographic data infected timing nature measures, including numbers tests quarantined individuals,...
Abstract Hand, foot and mouth disease (HFMD) is a major public health issue in Asia has global pandemic potential. Coxsackievirus A6 (CV-A6) was detected 514/2,230 (23%) of HFMD patients admitted to 3 hospitals southern Vietnam during 2011–2015. Of these patients, 93 (18%) had severe HFMD. Phylogenetic analysis 98 genome sequences revealed they belonged cluster A been circulating for 2 years before emergence. CV-A6 movement among localities within occurred frequently, whereas viral across...
Abstract We report a superspreading event of severe acute respiratory syndrome coronavirus 2 infection initiated at bar in Vietnam with evidence symptomatic and asymptomatic transmission, based on ministry health reports, patient interviews, whole-genome sequence analysis. Crowds enclosed indoor settings poor ventilation may be considered high risk for transmission.
ABSTRACT Enterovirus A71 (EV-A71) is a major cause of hand, foot, and mouth disease (HFMD) particularly prevalent in parts Southeast Asia, affecting thousands children infants each year. Revealing the evolutionary epidemiological dynamics EV-A71 through time space central to understanding its outbreak potential. We generated full genome sequences 200 strains sampled from various locations Viet Nam between 2011 2013 used these sequence data determine history phylodynamics Nam, providing...
Hand, foot and mouth disease (HFMD) continues to challenge Asia with pandemic potential. In Vietnam, there have been two major outbreaks occurring during 2011–2012 (>200,000 hospitalizations >200 deaths) more recently in 2018 (>130,000 17 deaths). Given the high burden complex epidemic dynamics of HFMD, synthesizing its clinical epidemiological data remains essential inform development appropriate interventions design public health measures. We report results a hospital-based study conducted...
Revealing the dispersal of dengue viruses (DENV) in time and space is central to understanding their epidemiology. However, processes that shape DENV transmission patterns at scale local populations are not well understood, particularly impact such factors as human population movement urbanization. Herein, we investigated trends spatial dynamics DENV-2 highly endemic setting southern Viet Nam. Through a phylogeographic analysis 168 full-length genome sequences obtained from hospitalized...
Understanding viral etiology and age-specific incidence of acute respiratory infections in infants can help identify risk groups inform vaccine delivery, but community-based data is lacking from tropical settings.One thousand four hundred seventy-eight urban Ho Chi Minh City 981 a semi-rural district southern Vietnam were enrolled at birth followed to 1 year age. Acute infection (ARI) episodes identified through clinic-based illness surveillance, hospital admissions self-reports....
In countries where malaria is endemic, its transmission a hazard of blood transfusion. The microscopical and immunological methods in current use for diagnosis are unsatisfactory low levels parasitaemia donations. polymerase chain reaction (PCR) can be 100-fold more sensitive than thick film examination when appropriate primers used detect distinguish Plasmodium falciparum P. vivax single tube. A study 1506 donations Ho Chi Minh City (3 which were positive) suggests that PCR provide an...
Central nervous system (CNS) infections are an important cause of childhood morbidity and mortality. The aetiologies these potentially vaccine-preventable have not been well established in Cambodia. We did a one year prospective study children hospitalised with suspected CNS infection at Angkor Hospital for Children, Siem Reap. Cerebrospinal fluid specimens (CSF) samples underwent culture, multiplex PCR serological analysis to identify range bacterial viral pathogens. Viral metagenomics was...
Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks severe and sometimes fatal hand, foot mouth disease (HFMD) across Asia-Pacific region. EV-A71 have been associated with (sub)genogroup switches, accompanied by recombination events. Understanding population dynamics is therefore essential for understanding this emerging infection, may provide pivotal information vaccine development. Despite public health burden EV-A71, relatively few complete-genome sequences...
The result of the study contributes to enhancing and sustaining future livelihoods food security in Vietnam vis-a-vis climate change. An innovative strategy based on marker-assisted backcrossing (MABC) was used transform popular rice variety AS996 into one can tolerate submergence while maintaining its original characteristics preferred by farmers consumers. tolerance QTL SUB1 counts for up 70% tolerant provides a marked improvement all genetic backgrounds environments tested so far....
Abstract Background Diagnosis of tuberculous meningitis (TBM) is hampered by the lack a gold standard. Current microbiological tests sensitivity and clinical diagnostic approaches are subjective. We therefore built model that can be used before test results known. Methods included 659 individuals aged $$\ge 16$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:mo>≥</mml:mo> <mml:mn>16</mml:mn> </mml:mrow> </mml:math> years with suspected brain infections from...
Abstract The first nationwide outbreak of COVID-19 in Vietnam started late April 2021 and was caused almost exclusively by a single Delta lineage, AY.57. In early 2022, multiple Omicron variants co-circulated with quickly became dominant. co-circulation happened leading to possibility co-infection recombination events which can be revealed viral genomic data. From January October total 1028 RNA samples out 4852 positive (Ct < 30) were sequenced the long pooled amplicons method on Illumina...
Background and objectives Treatment guidelines do not recommend antibiotic use for acute respiratory infections (ARI), except streptococcal pharyngitis/tonsillitis pneumonia. However, antibiotics are prescribed frequently children with ARI, often in absence of evidence bacterial infection. The this study were 1) to assess the appropriateness prescriptions mild ARI paediatric outpatients relation available detected pathogens, 2) on presentation using questionnaires detection urine 3) carriage...
ObjectivesCentral nervous system (CNS) infections are common causes of morbidity and mortality worldwide. We aimed to discover protein biomarkers that could rapidly accurately identify the likely cause infections, essential for clinical management improving outcome.MethodsWe applied liquid chromatography tandem mass spectrometry on 45 cerebrospinal fluid (CSF) samples from a cohort adults with without CNS potential diagnostic biomarkers. then validated performance selected biomarker...
Background Human respiratory syncytial virus ( RSV ) is an important community and nosocomial pathogen in developed countries but data regarding the importance of developing are relatively scarce. Methods During a 1‐year surveillance study 2010, we took serial samples from children admitted to Emergency Unit Respiratory Ward Children's Hospital 1 Ho Chi Minh City, Vietnam. was detected within 72 hours admission ward 26% (376/1439; A: n = 320; B: 54; A 2). Among those negative first after...
Real-time polymerase chain reaction (PCR) can be considered the gold standard for detection of influenza viruses due to its high sensitivity and specificity. Roche has developed RealTime ready Influenza A/H1N1 Detection Set, consisting a generic virus A PCR targeting M2 gene (M2 PCR) specific hemagglutinin (HA) A/H1N1-pdm09 (HA PCR, 2009 H1N1), with intention make reliable, rapid, simple test detect quantify H1N1 in clinical samples. We evaluated this kit against US Centers Disease Control...
ABSTRACT Point-of-care (POC) diagnostic tests for influenza can considerably shorten the time to clinical decision making. An investigational POC test based on a multiplexed immunoassay was developed by Meso Scale Diagnostics, LLC (MSD), with objective make more sensitive rapid that also subtype A viruses (1977 H1, H3, and H5). Between February November 2010, we conducted prospective multicenter study at four hospitals in Vietnam compared performance of this WHO/CDC real-time reverse...