A5085959637- Hematopoietic Stem Cell Transplantation
- Blood transfusion and management
- Blood groups and transfusion
- BRCA gene mutations in cancer
- Genetics, Bioinformatics, and Biomedical Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Hemoglobinopathies and Related Disorders
- Polyomavirus and related diseases
- Cancer Genomics and Diagnostics
- Mesenchymal stem cell research
- Transplantation: Methods and Outcomes
- Blood donation and transfusion practices
- Biomedical and Engineering Education
- Clinical Laboratory Practices and Quality Control
- Venous Thromboembolism Diagnosis and Management
- Erythrocyte Function and Pathophysiology
- Cytokine Signaling Pathways and Interactions
- Blood disorders and treatments
- Innovations in Medical Education
- Radiology practices and education
- Platelet Disorders and Treatments
- Clinical Reasoning and Diagnostic Skills
- Cancer-related gene regulation
- Kruppel-like factors research
- Health and Medical Research Impacts
Harvard University
2014-2024
Beth Israel Deaconess Medical Center
2014-2024
Montefiore Medical Center
2022
American Society for Clinical Pathology
2015-2018
Atkins (United States)
2018
Beth Israel Deaconess Hospital
2008-2016
University of Pennsylvania
2016
Massachusetts General Hospital
2005-2015
Stanford University
1993-2015
College of American Pathologists
2015
Umbilical cord blood (UBC) stem cells are a useful cell source for patients without matched related or unrelated donors. Adult transplantation with single UBC units is associated high transplantation-related mortality (TRM). In most cases, due to infection slow engraftment and immunoincompetence. this study, we used reduced-intensity conditioning regimen of fludarabine, melphalan, antithymocyte globulin followed by 2 partially units. The were 4/6 HLA match better each other the patient...
Obesity is an important public health problem associated with a number of disease states such as diabetes and arteriosclerosis. As such, understanding the mechanisms governing adipose tissue differentiation function considerable importance. We recently reported that Krüppel-like zinc finger transcription factor KLF15 can induce adipocyte maturation GLUT4 expression. In this study, we identify second family member, KLF2/Lung (LKLF), negative regulator differentiation. KLF2 highly expressed in...
The Stat1 activation-inactivation cycle involves phosphorylation of in the cytoplasm, translocation to nucleus, and then a return protein cytoplasm dephosphorylated state. However, intracellular site dephosphorylation has not been determined. As receptor signaling declines, flow activated molecules should be their dephosphorylation. We found that upon receptor-Janus kinase inactivation, either gradual or abruptly induced by staurosporine treatment, was from nucleus final compartment which...
T cell receptor (TCR) vaccination in rats prevents the development of experimental allergic encephalomyelitis (EAE), an animal model multiple sclerosis. The mechanism this potential immunotherapy was examined by vaccinating mice with immunogenic peptide fragment variable region TCR V beta 8.2 gene. Another immunogen that usually induces immune response mediated 8.2+ cells subsequently inhibited because specific clonal unresponsiveness (anergy) had been induced. Depletion CD8+ before blocked...
Background Blood transfusion is the most common hospital procedure performed in U nited S tates. While inadequate physician medicine knowledge may lead to inappropriate practice, such an educational deficit has not been investigated on international scale using a validated assessment tool. Identifying specific deficiencies critical for developing curricula improve patient care. Study Design and Methods Rasch analysis, method used high‐stakes testing, was validate tool consisting of...
Transforming growth factor (TGF)-β 1 is a pleiotropic with known inhibitory effects on immune cell activation. However, the specific mechanism(s) and in vivo significance of effectors TGF-β modulation context vascular inflammation are not well characterized. The chemokine monocyte chemoattractant protein (MCP)-1 critical for recruitment macrophages inflammatory disease states. In this study, we provide definitive evidence that ability to inhibit MCP-1 expression mediated via its effector...
As the 100th anniversary of Flexner report nears, medical student education is being reviewed at many levels. One area concern, expressed in recent reports from some national health care organizations, adequacy training discipline laboratory medicine (also termed clinical pathology). The Academy Clinical Laboratory Physicians and Scientists appointed an ad hoc committee to review this topic develop a suggested curriculum, which was subsequently forwarded entire membership for review....
There is evidence that physicians lack adequate transfusion medicine knowledge. To design needs-based educational interventions to address this gap, a validated assessment tool required. Previously published exams have not been created or using rigorous psychometric methods.A modified Delphi method was used achieve consensus regarding the essential knowledge and skills for who transfuse blood products. ensure content validity, members of an international organization experts (Biomedical...
Genomics and "medical sequencing" will revolutionize clinical laboratory diagnostics as the foundation for new era of personalized medicine. However, medical profession lags far behind technology business communities in recognizing preparing this change. Pathologists must take lead application genomics technologies, including whole-genome sequencing, to As a critical first step leading change, we have established first-in-the-nation resident curriculum Our goal is catalyze adoption similar...
Upon binding of gamma interferon (IFN-gamma) to its receptor, the latent transcription factor Stat1 becomes phosphorylated, dimerizes, and enters nucleus activate transcription. In response IFN-alpha, binds Stat2 in a heterodimer that recruits p48, an IRF family member, A number functional domains STATs, including C-terminal transactivation domain, dimerization SH2 are known. However, highly conserved residues between DNA (463 566), recently christened linker domain on basis crystallographic...
BACKGROUND: Although plasmapheresis has been recommended to reverse nephrotoxic elevations of serum free light chains (sFLCs), there are scant published data on removal sFLC measured with modern assays. STUDY DESIGN AND METHODS: levels were recorded in two patients myeloma‐associated renal failure receiving multiple procedures. RESULTS: In one patient, presenting acute 8 months after diagnosis κ‐LC myeloma, 16 procedures neither reduced (percent ranging from −70% +40%) nor improved function....
Double umbilical cord blood transplantation (UCBT) with a reduced-intensity conditioning regimen is an effective strategy for adult patients without matched donor. The risk of second malignancies in these has not yet been established, however. In the present study, 98 adults hematologic malignancy underwent double UCBT. Seventy received fludarabine 30 mg/m2/day 6 days, melphalan 100 1 day, and rabbit antithymocyte globulin 1.5 mg/kg/day 4 28 myeloablative total body radiation–containing...
BACKGROUND As transfusion is a common therapy and key component in every hematologist's practice, hematology training programs should dedicate significant time effort to delivering high‐quality medicine education their trainees. The current state of trainee knowledge not known. STUDY DESIGN AND METHODS A validated assessment tool developed by the Biomedical Excellence for Safer Transfusion (BEST) Collaborative was used assess prior education, attitudes, perceived ability, RESULTS total 149...
A small, but immunogenic dose of red blood cells (RBCs) may be contained in apheresis platelets (PLTs). Attempts are made to provide D- recipients with PLTs prevent anti-D alloimmunization and the potential for hemolytic disease fetus newborn. Beth Israel Deaconess Medical Center has a policy that when necessary transfuse D+ patients, we recommend RhIG given patient is woman child-bearing age or liver transplant patient. We sought retrospectively determine rate formation after D-incompatible...
Abstract Recommendations in the form of clinical practice guidelines are increasingly common. Clinical systematically developed statements designed to help administrators, practitioners and patients make decisions about appropriate health care for specific circumstances. In North America, by professional societies, government panels cooperative groups frequently used measure quality, allocate resources determine how dollars spent. For clinicians, provide a summary relevant medical literature...
Genomic medicine is transforming patient care. However, the speed of development has left a knowledge gap between discovery and effective implementation into clinical practice. Since 2010, Training Residents in Genomics (TRIG) Working Group found success building rigorous genomics curriculum with tools aimed at pathology residents postgraduate training years 1–4. Based on TRIG model, interprofessional Undergraduate (UTRIG) was formed. Under aegis Medical Educators Section Association...
Double-cord-blood transplantation (DCBT) offers an option for patients receiving reduced-intensity transplants. These unique transplants have two donors, both of whom are usually HLA mismatched at one to loci.Fifty-three were recipients a DCBT. Cords least 4/6 allele-level HLA-A, -B, and -DR match with the patient each other minimum combined cell dose more than 3.7 x 10(7) total nucleated cells per kg. Twenty-one received cyclosporine/mycophenolate mofetil 32 sirolimus/tacrolimus (SIR/TAC)...
Accumulating data suggest that team-based learning (TBL) is more effective than lecture-based teaching strategies. Educational sessions at national meetings, however, tend to be lecture-based, and unlike most examples of TBL, involve participants who do not know each other or the instructor.We evaluated a 1-day TBL genomic pathology workshop for residents held 3 meetings.A committee experts developed workshop. Prior attending, were provided access readings asked answer preparation questions....