A5074402443- Lipid Membrane Structure and Behavior
- Proteins in Food Systems
- Protein Structure and Dynamics
- Food composition and properties
- Supramolecular Self-Assembly in Materials
- Enzyme Structure and Function
- Polysaccharides Composition and Applications
- Food Chemistry and Fat Analysis
- biodegradable polymer synthesis and properties
- RNA Interference and Gene Delivery
- Advanced Cellulose Research Studies
- Graphene and Nanomaterials Applications
- Botanical Research and Chemistry
- Postharvest Quality and Shelf Life Management
- Surfactants and Colloidal Systems
- Food Drying and Modeling
- Plant Physiology and Cultivation Studies
- Microencapsulation and Drying Processes
- Antimicrobial Peptides and Activities
- Pickering emulsions and particle stabilization
- Phytase and its Applications
- Plant and fungal interactions
- Mercury impact and mitigation studies
- Lignin and Wood Chemistry
- Membrane Separation Technologies
Monash University
2021-2024
ETH Zurich
2019-2021
Charles Humbert 8
2020
The University of Melbourne
2015-2017
CSIRO Manufacturing
2014-2017
Biotechnology Institute
2017
Materials Science & Engineering
2014
Norwegian University of Science and Technology
2012
Lentils and mungbean proteins are under-researched compared to pea soybean. (green, red black-lentils), yellow protein isolates were obtained by alkaline extraction (pH 9)-isoelectric precipitation 4.5) investigated for molecular higher-order structures using complementary novel approaches. These extracted showed comparable content but significantly greater nitrogen solubility index (NSI > 85 %) than commercial soy < 60 %). Based on weight estimations from sodium dodecyl...
The molecular structure and conformation of the plant-based proteins dominate their gelation behaviour. These aspects have been mostly overlooked for underutilised pulse such as lentils mungbean that differ structurally with soy pea proteins. Rheological textural properties heat-induced gels (LPIs) (green, red black lentils), (MBPI) yellow (YPPI) were compared commercial (CSPI) (CPPI) protein isolates. On investigation dynamic rheological parameters, critical concentration (CPC) MBPI was...
The gelation behaviour of two different pea protein isolates and one soy isolate were investigated with a focus on the role properties. Protein solubility was lowest in pH 3 citrate-phosphate buffer (<10% w/w), increased 7.4 phosphate-buffered saline (12–21% highest 7.6 MilliQ water (~20–40% w/w). Heat-induced conditions for sources sensitive to both soluble insoluble fractions as obtained during extraction. At low concentrations (≤5% w/v), proteins started lose their viscoelastic exhibited...
Biomimetic cubic phases can be used for protein encapsulation in a variety of applications such as biosensors and drug delivery. Cubic with high concentration cholesterol phospholipids were obtained herein. It is shown that the phase structure maintained higher biomimetic membrane additives than has been reported previously. Opposing effects on curvature observed upon addition cholesterol. Furthermore, coronavirus fusion peptide significantly increased negative We show viral undergo...
Mungbean and lentil proteins are gaining attention for the production of high moisture extrusion (HME)-based meat- dairy-analogues. However, understanding mechanism fibrous structure formation molecular higher-order changes in HME remains limited. Globulin protein isolated from mungbean (MBPI), green (GLPI) yellow pea (YPPI) along with commercial soy (CSPI) (CPPI) isolates were investigated pasting properties using high-pressure rapid visco analyser (RVA), anisotropic structures at a...
In meso crystallization uses bicontinuous cubic lipidic mesophases as matrices for the of membrane proteins. this work, we look at impact a screen specifically marketed compatible with mesophase, Cubic (Emerald BioSystems), on formed by three different lipids: monoolein, monopalmitolein, and phytantriol. The was found to be mesophase retention under most conditions all lipids studied. effect individual components comprising multicomponent deconvoluted in two ways. Initially, specific...
Nanostructured bicontinuous lipidic cubic phases are used for the encapsulation of proteins in a range applications such as meso crystallization transmembrane and drug delivery vehicles. The retention nanoscale order subsequent to protein incorporation, well structure function, is essential all these applications. Herein synthetic peptides (WALP21, WALPS53, WALPS73) with common α-helical hydrophobic domain, but varying hydrophilic loop size, were designed systematically examine effect...
An understanding of the location peptides, proteins, and other biomolecules within bicontinuous cubic phase is crucial for evolving biological biomedical applications these hybrid biomolecule-lipid materials, including during in meso crystallization drug delivery. While theoretical modeling has indicated that proteins additive lipids might separate locally adopt a preferred phase, this never been experimentally confirmed. We have demonstrated perfectly contrast-matched phases D2O can be...
The membrane-spanning α helices of single-pass receptors play crucial roles in stabilizing oligomeric structures and transducing biochemical signals across the membrane. Probing intermolecular transmembrane interactions presents unique challenges, reflected a gross underrepresentation their membrane-embedded domains structural databases. Here, we present two high-resolution assemblies from eukaryotic protein crystallized lipidic membrane environment. Trimeric tetrameric immunoreceptor...
The interactions of viral fusion peptides from influenza (E4K and Ac-E4K) human immunodeficiency virus (gp41 Ac-gp41) with planar lipid bilayers monolayers was investigated herein. A combination surface-sensitive techniques, including quartz crystal microbalance dissipation (QCM-D), Langmuir-Blodgett area-pressure isotherms Micro-Brewster angle microscopy, neutron reflectometry, employed. Differences in the featuring ordered disordered phases, as well rafts, were revealed. HIV peptide (gp41)...
Lipidic bicontinuous cubic mesophases with encapsulated amphiphilic proteins are widely used in a range of biological and biomedical applications, including meso crystallization, as drug delivery vehicles for therapeutic proteins, biosensors biofuel cells. However, the effect protein encapsulation on phase nanostructure is not well-understood. In this study, we illustrate incorporating bacterial membrane Ag43, its individual hydrophobic β(43) hydrophilic α(43) domains, mesophases. For...
The problem of malnutrition and nutrition deficiency, as well droughts that lead to reduction in food supply starvation, is documented for Sub-Saharan Africa. Reducing post-harvest losses five species African leafy vegetables (ALVs) by preservation through drying studied herein. Energy efficient gentle conditions using superabsorbent polymers a temperature 40 °C were shown preserve most leaf structures vitamins. microbial safe moisture content the ALVs was found be ≤14% dry basis. Dried...
The proposed mechanism for in meso crystallization of transmembrane proteins suggests that a protein or peptide is initially uniformly dispersed the lipid self-assembly cubic phase but crystals grow from local lamellar phase, which acts as conduit between crystal and bulk phase. However, there very limited experimental evidence this theory. We have developed protocols to investigate mesophase microenvironment during growth using standard procedures readily available crystallography...
Abstract Coatings of anisotropic nanoparticles such as cellulose nanocrystals (CNCs) can provide tuneable physicochemical surface properties to a substrate modifying wettability. These coatings are often formed using dip coating, with CNCs enriched at the air–water interface transferred monolayer. This process is commonly facilitated by surfactants, which remain present in final product, affecting coating properties. In this work, an “additive free” method for creating CNC exploiting...
A fundamental understanding of the effect amphiphilic protein encapsulation on nanostructure bicontinuous cubic phase is crucial to progressing biomedical and biological applications these hybrid protein-lipid materials, including as drug delivery vehicles, biosensors, biofuel cells for in meso crystallization. The relationship between lipid nanomaterial encapsulated protein, however, remains poorly understood. In this study, we investigated incorporating five transmembrane lipo-proteins...
For evolving biological and biomedical applications of hybrid protein?lipid materials, understanding the behavior protein within lipid mesophase is crucial. After more than two decades since invention in meso crystallization method, a protein-eye view its mechanism still lacking. Numerous structural studies have suggested that integral membrane proteins preferentially partition at localized flat points on bilayer surface cubic phase with crystal growth occurring from local fluid lamellar L?...