A5069326278- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Bacillus and Francisella bacterial research
- SARS-CoV-2 detection and testing
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Immunotherapy and Immune Responses
- Computational Drug Discovery Methods
- Microbial Natural Products and Biosynthesis
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Porphyrin and Phthalocyanine Chemistry
- CAR-T cell therapy research
- Bacterial Infections and Vaccines
- Neuroscience and Neuropharmacology Research
- Viral Infections and Outbreaks Research
- Biochemical and Structural Characterization
- Cancer Immunotherapy and Biomarkers
- Photodynamic Therapy Research Studies
- CCD and CMOS Imaging Sensors
Institute of Microbiology
2022-2024
Chinese Academy of Sciences
2022-2024
University of Macau
2022-2024
University of Chinese Academy of Sciences
2023
West China Medical Center of Sichuan University
2023
Qinghai University
2022
Abstract Physical interactions between proteins are essential for most biological processes governing life 1 . However, the molecular determinants of such have been challenging to understand, even as genomic, proteomic and structural data increase. This knowledge gap has a major obstacle comprehensive understanding cellular protein–protein interaction networks de novo design protein binders that crucial synthetic biology translational applications 2–9 Here we use geometric deep-learning...
Emerging Omicron sub-variants are causing global concerns, and their immune evasion should be monitored continuously. We previously evaluated the escape of BA.1, BA.1.1, BA.2, BA.3 from an atlas 50 monoclonal antibodies (mAbs), covering seven epitope classes severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor-binding domain (RBD). Here, we update totally 77 mAbs against emerging including BQ.1.1 XBB find that BA.4/5, BQ.1.1, display further evasion. Besides, investigation...
Abstract Since SARS-CoV-2 Omicron variant emerged, it is constantly evolving into multiple sub-variants, including BF.7, BQ.1, BQ.1.1, XBB, XBB.1.5 and the recently emerged BA.2.86 JN.1. Receptor binding immune evasion are recognized as two major drivers for evolution of receptor domain (RBD) spike (S) protein. However, underlying mechanism interplay between factors remains incompletely understood. Herein, we determined structures human ACE2 complexed with XBB RBDs. Based on ACE2/RBD these...
The Omicron variant of SARS-CoV-2 carries multiple unusual mutations, particularly in the receptor-binding domain (RBD) spike (S) protein. Moreover, host-adapting such as residues 493, 498, and 501, were also observed RBD, which indicates that it is necessary to evaluate interspecies transmission risk variant. Herein, we evaluated recognition BA.1 Delta RBDs by 27 ACE2 orthologs, including humans. We found expanded its receptor binding spectra palm-civet, rodents, more bats (least horseshoe...
Abstract Multiple SARS-CoV-2 Omicron sub-variants, such as BA.2, BA.2.12.1, BA.4, and BA.5, emerge one after another. BA.5 has become the dominant strain worldwide. Additionally, BA.2.75 is significantly increasing in some countries. Exploring their receptor binding interspecies transmission risk urgently needed. Herein, we examine capacities of human other 28 animal ACE2 orthologs covering nine orders towards S proteins these sub-variants. The affinities between hACE2 sub-variants remain...
Immune checkpoint therapy (ICT) with a monoclonal antibody (MAb) against programmed cell death protein 1 (PD-1) is powerful clinical treatment for tumors. Cemiplimab human IgG4 approved in 2018 and the first MAb proven to be effective locally advanced basal carcinoma. Here, we report crystal structure of cemiplimab bound PD-1 effects N-glycosylation on interactions cemiplimab. The cemiplimab/PD-1 complex shows that mainly binds its heavy chain, whereas light chain serves as predominant...
Abstract The origin and host range of SARS-CoV-2, the causative agent coronavirus disease 2019 (COVID-19), are important scientific questions as they might provide insight into understanding potential future spillover to infect humans. Here, we tested binding between equine angiotensin converting enzyme 2 (eqACE2) receptor domains (RBDs) SARS-CoV, SARS-CoV-2 prototype (PT) variant concerns (VOCs), well their close relatives bat-origin (CoV) RaTG13 pangolin-origin CoVs GX/P2V/2017 GD/1/2019....
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has an extremely broad host range that includes hippopotami, which are phylogenetically closely related to whales. The cellular ACE2 receptor is one of the key determinants range. Here, we found ACE2s from several marine mammals and hippopotami could efficiently bind receptor-binding domain (RBD) both SARS-CoV SARS-CoV-2 facilitate transduction pseudoviruses into ACE2-expressing cells. We further resolved cryo-electron...
Thaumatin-like proteins (TLPs) participate in the defense responses of plants as well their growth and development processes, including seed germination. Yet functioning TLP family genes, addition to key details encoded protein products, has not been thoroughly investigated for Qingke ( Hordeum vulgare L. var. nudum ). Here, a total 36 genes were identified genome via HMM profiling. Of them, 25 TLPs contained signal peptide at N-terminus, with most predicted localize cytoplasm or outer...
Coronavirus disease 2019 , caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has a global pandemic.Intermediate horseshoe bats (Rhinolophus affinis) are hosts of RaTG13, the second most phylogenetically related viruses to SARS-CoV-2.We report binding between intermediate bat ACE2 (bACE2-Ra) and SARS-CoV-2 receptor-binding domain (RBD), supporting pseudotyped viral infection.A 3.3 Å resolution crystal structure bACE2-Ra/SARS-CoV-2 RBD complex was determined.The...
SARS-CoV-2 variants with severe immune evasion are a major challenge for COVID-19 prevention, especially the circulating Omicron XBB/BQ.1.1/BF.7 strains. Thus, next-generation of broad-spectrum vaccines urgently needed. Previously, we developed protein subunit vaccine, ZF2001, based on RBD-homodimer as immunogen. To adapt variants, chimeric RBD-heterodimers to induce broad responses. In this study, further explored concept tandem RBD homotrimer and heterotrimer. Prototype RBD-homotrimer,...
SARS-CoV-2 has been expanding its host range, among which the white-tailed deer (WTD), Odocoileus virginianus, became first wildlife species infected on a large scale and might serve as reservoir for variants of concern (VOCs) in case no longer circulating humans. In this study, we comprehensively assessed binding WTD angiotensin-converting enzyme 2 (ACE2) receptor to spike (S) receptor-binding domains (RBDs) from prototype (PT) strain multiple variants. We found that ACE2 could be broadly...
Foxes are susceptible to SARS-CoV-2 in laboratory settings, and there have also been reports of natural infections both SARS-CoV foxes. In this study, we assessed the binding capacities fox ACE2 important sarbecoviruses, including SARS-CoV, SARS-CoV-2, animal-origin related viruses. Our findings demonstrated that exhibits broad capabilities receptor-binding domains (RBDs) sarbecoviruses. We further determined cryo-EM structures complexed with RBDs prototype (PT), Omicron BF.7. Through...
Abstract The continual emergence and circulation of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have caused a great challenge for the disease 2019 (COVID-19) pandemic control. Recently, Omicron BA.2.86 was identified with more than 30 amino acid changes on spike (S) protein, compared to BA.2 or XBB.1.5. immune evasion potential is concern. In this study, we evaluated neutralizing activities sera collected from participants mice. Participants were divided into...
Abstract Physical interactions between proteins are essential for most biological processes governing life. However, the molecular determinants of such have been challenging to understand, even as genomic, proteomic, and structural data grows. This knowledge gap has a major obstacle comprehensive understanding cellular protein-protein interaction (PPI) networks de novo design protein binders that crucial synthetic biology translational applications. We exploit geometric deep learning...
Photodynamic therapy has emerged as a recognized anti-tumor treatment involving three fundamental elements: photosensitizers, light, and reactive oxygen species. Enhancing the effectiveness of photosensitizers remains primary avenue for improving biological therapeutic outcomes PDT. Through generations development, HPPH is 2-(1-hexyloxyethyl)-2-devinyl derivative pyropheophorbide-α, representing second-generation photosensitizer already undergoing clinical trials various tumors. The...
The potential host range of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been expanding alongside its evolution during the pandemic, with rabbits and hares being considered important hosts, supported by a report rabbit sero-prevalence in nature. We measured binding affinities hare angiotensin-converting enzyme (ACE2) receptor-binding domains (RBDs) from SARS-CoV, SARS-CoV-2, variants found that ACE2s had broad variant tropism, significantly enhanced to Omicron BA.4/5...
With additional mutations related to BA.2, the newly circulating Omicron sub-variants, BA.2.12.1, BA.2.13, BA.4 and BA.5, further draw global concerns. Thus, their immune evasion should be monitored immediately. We previously systematically evaluated escape of BA.1, BA.1.1, BA.2 BA.3 atlas 50 human monoclonal antibodies (mAbs), covering seven epitope classes SARS-CoV-2 receptor-binding domain (RBD). Here, we updated existing antibody against BA.5 (BA.4/5) found that BA.2.12.1 BA.2.13...