This study investigated the effects of bilateral injections serotonergic receptor agonist and antagonist into lateral parabrachial nucleus (LPBN) on ingestion water 0.3 M NaCl induced by intracerebroventricular angiotensin II (ANG II) or combined subcutaneous diuretic furosemide (Furo) angiotensin-converting enzyme inhibitor captopril (Cap). Rats had stainless steel cannulas implanted bilaterally LPBN left ventricle. Bilateral pretreatment with 5-HT1/5-HT2 methysergide (4 micrograms/200 nl...

Leptin, an adipocyte-derived hormone, is suggested to participate in the central control of breathing. We hypothesized that leptin may facilitate ventilatory responses chemoreflex activation by acting on respiratory nuclei ventrolateral medulla. The baseline ventilation and CO2 were evaluated before after daily injections into retrotrapezoid nucleus/parafacial group (RTN/pFRG) for 3 days obese leptin-deficient (ob/ob) mice.

This study investigated the effects of bilateral injections a serotonin (5-HT) receptor agonist into lateral parabrachial nucleus (LPBN) on intake NaCl and water induced by 24-h deprivation or sodium depletion followed 24 h (injection diuretic furosemide plus sodium-deficient diet). Rats had stainless steel cannulas implanted bilaterally LPBN. Bilateral LPBN serotonergic 5-HT 1/2 antagonist methysergide (4 μg/200 nl at each site) increased hypertonic when tested after deprivation. Water also...

This study investigated the roles of serotonin (5-HT) receptors in lateral parabrachial nucleus (LPBN) and brain angiotensin type 1 (AT1) intake 0.3 M NaCl water induced by II (ANG II). Rats were implanted with stainless steel cannulas for injections into subfornical organ (SFO) LPBN. Bilateral LPBN pretreatment nonselective serotonergic 5-HT1/5-HT2 receptor antagonist methysergide (4 micrograms/200 nl) markedly enhanced ANG (20 ng/200 SFO. Pretreatment SFO AT1 losartan (1 microgram/200...

This study investigated the effects of bilateral injections serotonergic receptor ligands into lateral parabrachial nucleus (LPBN) on pressor and dipsogenic responses induced by intracerebroventricular (i.c.v.) injection angiotensin II (ANG II). Rats with stainless steel cannulas implanted bilaterally LPBN left ventricle were used to i.c.v. ANG II-induced water intake responses. Pretreatment 5-HT1/5-HT2 antagonist methysergide (1-8 micrograms/200 nl) injected increased (50 ng/microliters)...

In the present study we investigated whether interruption of chemoreceptor reflex by an electrolytic lesion commissural subnucleus nucleus tractus solitarii (commNTS) influenced pressor and bradycardic responses induced microinjection L-glutamate (L-Glu) into medial NTS (mNTS) conscious rats. Seven days after sham lesions, seven rats demonstrated significant [change in mean arterial pressure (MAP) = +33 +/- 3 mmHg] heart rate (HR) -74 8 beats/min (bpm)] to activation intravenous injection...

Expansion of the blood volume causes a release atrial natriuretic peptide (ANP) that is believed to be important in induction subsequent natriuresis and diuresis which, turn, acts reduce increase volume. Since stimulation anteroventral portion third cerebral ventricle (AV3V) induced rapid elevation plasma ANP, whereas lesions AV3V were followed by marked decline concentration peptide, we hypothesized ANP from brain neuronal system might control ANP. The perikarya ANP-containing neurons are...

A rise in arterial Pco(2) stimulates breathing and sympathetic activity to the heart blood vessels. In present study, we investigated involvement of retrotrapezoid nucleus (RTN) glutamatergic mechanisms Bötzinger/C1 region (Bötz/C1) these responses. Splanchnic nerve discharge (sSND) phrenic (PND) were recorded urethane-anesthetized, sino-aortic-denervated, vagotomized, artificially ventilated rats subjected hypercapnia (end-expiratory CO(2) from 5% 10%). Phrenic was absent at end-expiratory...

Renal denervation (RDN) is a potential therapy for drug-resistant hypertension. However, whether its effects are mediated by ablation of efferent or afferent renal nerves not clear. Previous studies have implicated that inflammation and the sympathetic nervous system driven activation nerves. RDN attenuated activity in some animal models In 2 kidney,1 clip (2K1C) model renovascular hypertension, also decreased activity; however, mechanisms underlying central still unclear. We tested...

Obesity is increasingly prevalent worldwide, and climate change exacerbating water shortages, leading to dehydration. Both obesity dehydration cause increased arterial pressure (AP), fluid electrolytic imbalance, neuroinflammation. Thus, the present study aimed verify changes in cardiovascular system, hydroelectrolytic balance microglia neuronal activation rats fed with a high-fat diet (HFD) response 24 h of deprivation (WD) possible mechanisms involved. Male Holtzman (290-310 g) were...

10.1152/ajpregu.00295.2000. Adult rats deprived of water for 24–30 h were allowed to rehydrate by ingesting only 1–2 h. Rats then given access both and 1.8% NaCl. This procedure induced a sodium appetite defined the operational criteria significant increase in NaCl intake (3.8 ± 0.8 ml/2 h; n = 6). Expression Fos (as assessed immunohistochemistry) was increased organum vasculosum lamina terminalis (OVLT), median preoptic nucleus (MnPO), subfornical organ (SFO), supraoptic (SON) after...

Stimulation of the region antero-ventral to third cerebral ventricle (AV3V) by a cholinergic drug, carbachol, and lesions AV3V have been demonstrated in previous studies either augment or decrease sodium excretion, respectively. Atrial natriuretic peptide (ANP) dramatically increases renal excretion has localized brain areas previously shown be involved control excretion. Consequently, evaluate possible role ANP evoking changes that follow stimulations AV3V, we determined effect injection...

Both systemically administered furosemide and isoproterenol produce water intake (i.e., thirst). Curiously, however, in light of the endocrine hemodynamic effects produced by these treatments, they are remarkably ineffective eliciting hypertonic saline solutions operationally defined as sodium appetite). Recent work indicates that bilateral injections serotonin receptor antagonist methysergide into lateral parabrachial nuclei (LPBN) markedly enhance a preexisting appetite. The present...

The present study investigated the effects of bilateral injections nonselective CCK receptor antagonist proglumide or CCK-8 into lateral parabrachial nuclei (LPBN) on ingestion 0.3 M NaCl and water induced by intracerebroventricular injection ANG II a combined treatment with subcutaneous furosemide (Furo) + captopril (Cap). Compared saline (vehicle), LPBN (50 μg ⋅ 200 nl −1 site ) increased intake ng/1 μl). Bilateral also II-induced when was simultaneously available, but not only present....

The nucleus tractus solitarius (NTS), a major hindbrain area involved in cardiovascular regulation, receives primary afferent fibers from peripheral baroreceptors and chemoreceptors. Hydrogen peroxide (H(2)O(2)) is relatively stable diffusible reactive oxygen species (ROS), which acting centrally, may affect neural mechanisms. In the present study, we investigated effects of H(2)O(2) alone or combined with glutamatergic antagonist kynurenate into NTS on mean arterial pressure (MAP) heart...

Melanocortin receptors (MC3/4R) mediate most of the metabolic and cardiovascular actions leptin.Here, we tested if MC4R also contributes to leptin's effects on respiratory function.After control measurements, male Holtzman rats received daily microinjections leptin, SHU9119 (MC3/4R antagonist) or combined with leptin infused into brain lateral ventricle for 7 days. On 6th day treatment, tidal volume (VT ), frequency (fR ) pulmonary ventilation (VE were measured by whole-body plethysmography...