The physicochemical characteristics of nanostructured suspensions are important prerequisites for the success new drug development. This work aimed to develop nanometric systems containing Cymbopogon densiflorus leaf essential oil and evaluate their antimicrobial activity. was isolated by hydrodistillation from leaves analyzed GC-MS. main constituents were found be trans-p-mentha-2,8-dien-1-ol, cis-p-mentha-2,8-dien-1-ol, trans-p-mentha-1(7),8-dien-2-ol, cis-piperitol,...

The relationship between enteropathogens and severe diarrhoea in the Brazilian Amazon is poorly understood. In 1998, outbreaks of acute clinically diagnosed as cholera occurred two small villages localized far from main route rainforest. PCR was performed on some heat-labile (LT) and/or heat-stable (STh) toxin genes, virulence determinants enterotoxigenic Escherichia coli (ETEC), were detected. Further characterization ETEC isolates revealed presence clones, one each outbreak. One presenting...

ABSTRACT In this study, we demonstrated that analyzed strains of Vibrio mimicus and cholerae could be separated in two groups by using multilocus enzyme electrophoresis (MEE) data from 14 loci. We also showed the combination four enzymatic loci enables us to differentiate these species. Our results ribosomal intergenic spacer regions PCR-mediated identification system failed, some cases, between V. . On other hand, MEE proved a powerful molecular tool for discrimination species even when...

Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they encapsulate hydrophobic substances. We developed characterized micellar formulations containing resveratrol evaluated their cytotoxic antimicrobial effects. The were prepared by the cold dispersion method with different...

To investigate whether medical devices coated with a synthesized nanocomposite of poly(methylmethacrylate-co-dimethyl acrylamide) (PMMDMA) and silver nanoparticles (AgNPs) could improve their antibiofilm antimicrobial activities. We also investigated the nanocomposite's safety.

The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial cytotoxicity assays. Compounds the most active fraction identified by GC-MS LC-MS. CEE was against 19 bacteria ethyl acetate (EAF) showed lowest minimum bactericidal concentration (MBC 0.3–80.0 mg/mL). Through time-kill assay observed that EAF induced rapid effect Staphylococcus saprophyticus. tests L929 fibroblasts great potential on treatment infections caused five < IC50)....

The aim of this work was to determine the total phenolic content and evaluate antioxidant, antibacterial, photoprotective cytotoxic properties corn silk ethanol extract prepared from that air-dried at room temperature. Corn has a high compounds. concentrations required produce 50% maximal effect (EC50) in DPPH ABTS radical scavenging assays were 489.0 166.1 µg/mL respectively, indicating low activity when compared positive control. ethanolic showed antibacterial activity, mainly on...

The treatment of bladder cancer remains a challenge in clinical practice. Different chemotherapeutic protocols can be used; however, it is common to observe tumor recurrence and secondary effects that result toxicity. Doxorubicin (DOX), one the most effective anticancer agents used treat cancer, cause chronic cardiotoxicity, limiting its use Resveratrol (RES), natural product with potential antitumor activity against associated rapid metabolism low bioavailability needs combined drugs...

Resumo Fundamento Genes e suas variantes associadas a fatores ambientais contribuem para o desenvolvimento do fenótipo hipertenso. O gene da subunidade beta 3 proteína G ( GNB3 ) está envolvido no processo de sinalização intracelular têm sido relacionadas à suscetibilidade hipertensão arterial. Objetivo Determinar associação variante (rs5443:C>T) com arterial, parâmetros bioquímicos, idade obesidade em indivíduos hipertensos normotensos Ouro Preto, Minas Gerais. Método A identificação das foi [...]

Background and Objectives: Bacterial vaginosis (BV) is the most common cause of vaginal discharge in world. The study aimed to estimate prevalence identify risk factors associated with bacterial vaginosis. Methods: A cross-sectional was conducted Ouro Preto, Brazil, between February December 2017. Three hundred forty-one women aged 18 years or older, users Brazilian Unified Health System, participated this study. Women who used oral topical antibiotics four weeks prior sample collection had...

The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian Cerrado, whose ethanolic extract showed significant vitro anti-Zika virus activity by MTT colorimetric method. Currently, there no drug clinical use specifically for treatment of this virus; therefore, work, antiviral and cytotoxic properties extract, fractions, compounds were evaluated. leaves cytotoxicity human MRC-5 cell was moderately Vero (CC50 161.5 ± 2.01 µg/mL). This inhibited Zika...

A crescente incidência de patógenos resistentes aos medicamentos atuais incentiva a busca novos agentes antimicrobianos. Neste contexto, as plantas medicinais se destacam, sendo uma importante fonte fármacos. Existem diversos métodos para avaliar atividade antibacteriana e antifúngica extratos, frações, óleos essenciais substâncias isoladas vegetais. Os mais conhecidos incluem difusão, diluição bioautografia. proposta desse trabalho é apresentar os utilizados atualmente, juntamente com suas...

Due to the increase of bacterial resistance, search for new antibiotics is necessary and medicinal plants represent its most important source. The aim this study was evaluate antibacterial property extract fractions from Protium spruceanum leaves, against pathogenic bacteria. By means diffusion microdilution assays, crude active nine bacteria tested being hydromethanolic fraction active. During phytochemical procedures, procyanidin (1) catechin (2) were identified as main constituents...

Modified release systems depend on the selection of an appropriate agent capable controlling drug, sustaining therapeutic action over time, and/or releasing drug at level a particular tissue or target organ. Polyethylene glycol 4000 (PEG 4000) is commonly employed in formulations while polymethyl methacrylate (PMMA) non-toxic and has good solubility organic solvents. This study aimed incorporation ketoconazole PMMA-g-PEG its derivatives, thus evaluating profile anti-Candida albicans...

The treatment of skin and soft tissue infections (SSTIs) can be challenging due to bacterial resistance, particularly from strains like MRSA biofilm formation. However, combining conventional antibiotics with natural products shows promise in treating SSTIs. objective this study is develop a nanoemulsion-based hydrogel containing Protium spruceanum extract mupirocin evaluate its potential for the nanoemulsion was obtained by phase inversion subsequently characterized. antibacterial activity...

Abatia americana is a Brazilian species that has yet to be the subject of scientific investigation. However, it represents potential source new antibacterial and anticancer drugs, given belongs Salicaceae family. The objective this study was investigate biological activities chemical composition species. characterisation conducted using UPLC-QTOF-MS/MS. cytotoxicity evaluated sulforhodamine B method activity determined through microdilution assay. Within fractions obtained, namely hexane...

A água representa o principal meio de transmissão micro-organismos em centros saúde, os quais podem causar infecções que são um grave problema saúde pública. O objetivo desse estudo foi avaliar a qualidade microbiológica da utilizada hospital microrregião Ouro Preto, Minas Gerais, Brasil. Foram coletadas doze amostras pontos distintos para testes detecção coliformes totais e termotolerantes, assim como outros microrganismos; além disso, sensibilidade antimicrobianos também foram realizados....

Synthetic polymers have been extensively studied in several areas of knowledge, one which is the pharmaceutical.The drug when encapsultated into PMMA-g-PEG 4000 matrix can produce fewer side effects.The most frequent effects erythromycin are gastrointestinal and dose related.Nausea, vomiting diarrhea occur low frequency with usual oral doses.The used this study was Erythromycin, an antimicrobial that acts directly on bacterial protein synthesis.Erythromycin encapsulated derivatives.All...

Candida albicans (C. albicans) are the most common cause of urinary fungal infections. C. biofilms increasing clinical importance due to their resistance antifungal therapy. Since use medical devices causes hospital infections, polymeric coatings that reduce microorganisms adhesion and biofilm formation considered an attractive strategy. In this work, ability possible mechanisms poly(methylmethacrylate-co-dimethylacrylamide) (PMMDMA) inhibit on have been studied. Scanning electron microscopy...

Aim: We examined whether ADIPOQ (rs266729 and rs1501299) NOS3 (rs3918226 rs1799983) SNPs or the haplotypes formed by them, affect blood pressure (BP) control in 196 patients with adherence to antihypertensive therapy grouped into controlled (BP <140/90 mmHg) uncontrolled ≥140/90 hypertension. Materials & methods: The average of three most recent BP measurements was retrieved from patients' electronic medical records. Adherence evaluated using Morisky–Green test. Haplotype frequencies were...