A5100746012- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Sphingolipid Metabolism and Signaling
- Caveolin-1 and cellular processes
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- MicroRNA in disease regulation
- Photosynthetic Processes and Mechanisms
- Single-cell and spatial transcriptomics
- Protein Kinase Regulation and GTPase Signaling
- Biochemical Analysis and Sensing Techniques
- Plant-derived Lignans Synthesis and Bioactivity
- Developmental Biology and Gene Regulation
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Microbial bioremediation and biosurfactants
- Plant Molecular Biology Research
- Immunotherapy and Immune Responses
- Plant Taxonomy and Phylogenetics
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Central South University
2023-2024
Hunan Cancer Hospital
2024
Shanghai University
2016-2024
State Key Laboratory of Natural and Biomimetic Drugs
2020-2023
Peking University
2014-2023
Henan Agricultural University
2023
Jilin Agricultural University
2023
Shandong University of Science and Technology
2022
University of South China
2020-2022
Significance Monoterpenoids, monoterpene indole alkaloids, and cannabinoids are highly valued for their fragrant therapeutic properties, but sourcing them from nature or deriving petrochemicals is no longer sustainable. However, sustainable production of these compounds in engineered microorganisms mostly hampered by the limited availability main building block biosynthesis, geranyl diphosphate. Here, we overcome this challenge engineering yeast peroxisomes as diphosphate-synthesizing...
Crucihimalaya himalaica, a close relative of Arabidopsis and Capsella, grows on the Qinghai-Tibet Plateau (QTP) about 4,000 m above sea level represents an attractive model system for studying speciation ecological adaptation in extreme environments. We assembled draft genome sequence 234.72 Mb encoding 27,019 genes investigated its origin adaptive evolutionary mechanisms. Phylogenomic analyses based 4,586 single-copy revealed that C. himalaica is most closely related to Capsella (estimated...
Sphingosine kinase (SphK)/sphingosine-1-phosphate (S1P)/S1P receptor (S1PR) signaling pathway has been implicated in a variety of pathological processes ovarian cancer. However, the function this axis cancer angiogenesis remains incompletely defined. Here we provided first evidence that SphK1/S1P/S1PR1/3 played key roles angiogenesis. The expression level SphK1, but not SphK2, was closely correlated with microvascular density (MVD) tissue. In vitro, angiogenic potential and factor secretion...
Δ12-fatty acid dehydrogenase (FAD2) is the essential enzyme responsible for catalyzing formation of linoleic from oleic acid. CRISPR/Cas9 gene editing technology has been an tool molecular breeding in soybeans. To evaluate most suitable type soybean fatty synthesis metabolism, this study selected five crucial genes FAD2 family—GmFAD2-1A, GmFAD2-1B, GmFAD2-2A, GmFAD2-2B, and GmFAD2-2C—and created a CRISPR/Cas9-mediated single vector system. The results Sanger sequencing showed that 72...
Background: The Influenza A virus (IAV), a pathogen affecting the respiratory system, represents major risk to public health worldwide. Immunization remains foremost strategy control transmission of IAV. has two primary antigens: hemagglutinin (HA) and neuraminidase (NA). Our previous studies have demonstrated that an IAV NA mRNA vaccine can induce Th1-type immune responses in mice. This research examined elicited by targeting both HA antigens murine models. Methods: In this study, we used...
Gene therapy offers potentially transformative strategies for major human diseases. However, one of the key challenges in gene is developing an effective strategy that could deliver genes into specific tissue. Here, we report a novel virus-like nanoparticle, bioorthgonal engineered recombinant biosome (reBiosome), efficient therapies cancer and inflammatory The mutant (mBiosome) first prepared by site-specific codon mutation displaying 4-azido-L-phenylalanine on vesicular stomatitis virus...
Nasopharyngeal carcinoma (NPC) is a common malignant tumor of the head and neck, prevalent in regions such as Southern China Southeast Asia. Radiotherapy serves primary clinical treatment for this carcinoma. However, resistance to radiotherapy fundamental cause failure patient mortality, with underlying mechanisms yet be fully elucidated. We identified recently characterized circular RNA, circADARB1, which markedly upregulated NPC tissues closely associated poor prognosis resistance. Both...
This paper studies the effects of international taxation on multinational firms’ production outsourcing strategies, which has attracted recent attention from both practitioners and legislators. We formulate a stylized economic model with information asymmetry find that under territorial tax system, presence global disparity may result in subtle changes firm's strategy. In particular, consideration can make firm more inclined to procure materials by its overseas subsidiary then resell...
Intramuscular fat (IMF) content is the major indicator for evaluating chicken meat quality due to its positive correlation with tenderness, juiciness, and flavor. An increasing number of studies are focusing on functions microRNAs (miRNAs) in intramuscular adipocyte differentiation. However, little known about association miR-128-3p Our previous RNA-seq results indicated that was differentially expressed at different periods adipocytes, revealing a possible adipogenesis. The purpose this...
Swanson's literature-based discovery focus on resurrecting previously published but neglected knowledge. In this study, we propose a two-step model of the process and generate hypothesis between anandamide gastric cancer. Further, potential relationship was confirmed by follow-up experimentation. The treatment resulted in cell cycle redistribution cancer cells. Most importantly, variation mediated some genes from B-terms closed discovery, indicating role B-terms. not only collates data for...
Estrogen receptor-positive (ER+) breast cancer accounts for the majority of cancers diagnosed, and nearly 20% patients do not respond to endocrine therapy. The pathogenesis ER+ has been well elucidated. enhancer is a cis-regulatory element that promotes gene transcription plays an important role in spatiotemporal expression cellular genes. Nevertheless, oncogenic its occurrence progression remain unclear. Here, we report novel (named αE myc ) c-Myc reveal activation mechanism cancer. results...
Long non-coding RNAs (lncRNAs) are a class of RNA with length greater than 200 nt. It has mRNA-like structure, formed by splicing after transcription, and contains polyA tail promoter, whom promoter plays role binding transcription factors. LncRNAs’ sequences low in conservation, other species can only find handful the same lncRNAs as humans, there different ways during differentiation identical species, spatiotemporal expression specificity. With developing high-throughput sequencing...
In the development and evolution of international trade theory, comparative advantage has always been a core concept. A great deal research pertains to calculation methods advantage. However, most previous on measurement is mainly based country's import/export volume specific industry or product. Under circumstances contemporary intra-product specialization, are not appropriate. It imperative improve original measure through stripping overseas contents exports, putting forward new index...
Abstract Dorsoventral (DV) embryonic patterning relies on precisely controlled interpretation of morphogen signaling. In all vertebrates, DV axis specification is informed by gradients Bone Morphogenetic Proteins (BMPs). We developed a 3D single-molecule mRNA quantification method in whole-mount zebrafish to quantify the inputs and outputs this pathway. combination with computational modeling embryo development, data from revealed that Sizzled (Szl), shaped BMP Nodal signaling, kept...
Abstract Background Nasopharyngeal carcinoma (NPC) is a prevalent head and neck malignancy in Southeast Asia. Radiotherapy the primary clinical treatment for NPC, but radiotherapy resistance stands as fundamental reason failure patient mortality. Nevertheless, mechanism underlying NPC remains elusive. Methods The expression of circADARB1 tissues was examined by quantitative real-time polymerase chain reaction situ hybridization. Colonies assay, MTT, ROS lipid peroxidation level measurement,...
Ubiquitin is a small, 76‐amino acid polypeptide that can be covalently attached to other proteins regulate their activities. The role of ubiquitin in tagging for proteasome‐mediated degradation its most well‐known function, but regulates non‐proteolytic processes as well. Ubiquitin's versatility due, part, by ability linked into different types polymers, depending on which seven lysine connect the individual subunits together. Polymers assembled through ubiquitin's 48 are generally...
The decline of male fertility and its consequences on human populations are important public-health issues. However, there limited choices for treatment infertility. In an attempt to identify a compound that could promote fertility, we identified characterized library small molecules from ancient formulation Wuzi Yanzong-Pill, which was used as folk medicine since the Tang dynasty China. We found SB enabled evident repairs in oligoasthenospermia-associated testicular tissue abnormality...
Abstract Background: Epithelial ovarian cancer (EOC) is the leading cause of death from female cancers. In our previous study, Sphingosine kinase 2 (SphK2) inhibitor was shown to display anti-EOC activities. The purpose this study further evaluate expression characteristics and clinical significance SphK2 in EOC, explore roles underlying mechanisms EOC cell survival. Methods: examined by Immunohistochemistry western blot, its implications prognostic were analyzed. Cellular proliferation...
Under the complex marine environment conditions, petroleum degrading bacteria could not be guaranteed to remediate offshore oil-contaminated areas effectively. Besides, continuous-running bioreactor for degradation was rarely mentioned in studies. In this paper, a full-filled with immobilized beads designed further research. The results indicated rate of remain above 50% during former weeks whereas performance decreased bioremediation time. Intriguingly, diameter reduced by 32.49% after...
Abstract Dorsoventral (DV) embryonic patterning relies on precisely controlled interpretation of morphogen signaling. In all vertebrates, DV axis specification is informed by gradients bone morphogenetic proteins (BMPs). We developed a 3D single-molecule mRNA quantification method in whole-mount zebrafish to quantify the inputs and outputs this pathway. combination with computational modeling embryo development, data from revealed that sizzled (Szl), shaped BMP Nodal signaling, maintained...