A5101964043- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Autoimmune and Inflammatory Disorders Research
- Periodontal Regeneration and Treatments
- Oral and gingival health research
- Adolescent and Pediatric Healthcare
- Rheumatoid Arthritis Research and Therapies
- MicroRNA in disease regulation
- Oral microbiology and periodontitis research
- Oral and Maxillofacial Pathology
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Plant-derived Lignans Synthesis and Bioactivity
- Barrier Structure and Function Studies
- Dendrimers and Hyperbranched Polymers
- Sperm and Testicular Function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Neuropeptides and Animal Physiology
- Phagocytosis and Immune Regulation
- Extracellular vesicles in disease
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
Peking University
2016-2025
National Clinical Research Center for Digestive Diseases
2023-2025
Second Affiliated Hospital of Dalian Medical University
2022-2025
Dalian Medical University
2022-2025
State Key Laboratory of Natural and Biomimetic Drugs
2019-2024
King University
2024
Stomatology Hospital
2014-2023
Shenyang University of Chemical Technology
2023
Pain and Rehabilitation Medicine
2021
Guangzhou Medical University
2012-2020
Natural exosomes can express specific proteins and carbohydrate molecules on the surface hence have demonstrated great potentials for gene therapy of cancer. However, use natural is restricted by their low transfection efficiency. Here, we report a novel targeting tLyp-1 exosome recombinant engineering delivery siRNA to cancer stem cells. To reach such purpose, engineered tLyp-1-lamp2b plasmids were constructed amplified in Escherichia coli. The further used transfect HEK293T tool cells...
Cancer patients by immune checkpoint therapy have achieved long-term remission, with no recurrence of clinical symptoms cancer for many years. Nevertheless, more than half are not responsive to this due exhaustion. Here, we report a novel gene engineered exosome which is rationally designed engineering PD1 and simultaneously enveloping an adjuvant imiquimod (PD1-Imi Exo) boosting response blockage therapy. The results showed that PD1-Imi Exo had vesicular round shape (approximately 139 nm),...
A dual-mediated liposomal drug delivery system targeting the brain: rational construction, integrity evaluation across blood-brain barrier, and transporting mechanism to glioma cells Chang Liu,1,* Xiao-Na Gui-Ling Wang,1 Yu Hei,1 Shuai Meng,1 Ling-Fei Yang,1 Lan Yuan,2 Ying Xie1 1Beijing Key Laboratory of Molecular Pharmaceutics New Drug Delivery Systems, Department Pharmaceutics, School Pharmaceutical Sciences, 2Medical Healthy Analysis Center, Peking University, Beijing, People’s Republic...
Abstract: Cell-penetrating peptides (CPPs) as small molecular transporters with abilities of cell penetrating, internalization, and endosomal escape have potential prospect in drug delivery systems. However, a bottleneck hampering their application is the poor specificity for cells. By utilizing function hydration shell polyethylene glycol (PEG) acid sensitivity hydrazone bond, we constructed kind CPP-modified pH-sensitive PEGylated liposomes (CPPL) to improve selectivity these tumor...
Background and Aims: Accurately predicting the response to methotrexate (MTX) in juvenile idiopathic arthritis (JIA) patients before administration is key point improve treatment outcome. However, no simple reliable prediction model has been identified. Here, we aimed develop validate predictive models for MTX JIA using machine learning based on electronic medical record (EMR) after administering MTX. Materials Methods: Data of 362 with mono-therapy were retrospectively collected from EMR...
Background: Neo-adjuvant chemotherapy is an effective strategy for improving treatment of breast cancers. However, the efficacy this limited triple negative cancer (TNBC). Gene therapy may be a more prognosis TNBC. Methods: A novel 25 nucleotide sense strand miRNA was designed to treat TNBC by silencing Slug gene, and encapsulated into DSPE-PEG2000-tLyp-1 peptide-modified functional liposomes. The liposomes evaluated on invasive cells cancer-bearing nude mice. Furthermore, vinorelbine were...
Perfluorooctanesulfonate (PFOS), an emerging contaminant with widespread concern, has been associated the pathogenesis of atherosclerosis (AS). As a substitute for PFOS, sodium p-perfluorous nonenoxybenzenesulfonate (OBS) is extensively utilized in various applications and detected human blood. However, its potential health risk AS remain unclear. In this study, we investigated comparative impacts PFOS OBS on endothelial dysfunction atherogenesis. vivo Apolipoprotein E knockout (ApoE–/–)...
To compare the clinical, esthetic, and patient-reported outcomes of vestibular incision subperiosteal tunnel access (VISTA) technique modified (mTunnel) technique, both combined with connective tissue grafts (CTGs), for treating recession type 1 (RT1) single gingival recessions. A total 24 patients non-molar RT1 recessions were randomly assigned to VISTA + CTG or mTunnel group. blinded examiner assessed reduction (RecRed), mean root coverage (MRC), complete (CRC), keratinized width,...
Immune checkpoint blockade therapy using programmed cell death 1 (PD1) or ligand (PD-L1) has made significant progress in the treatment of advanced cancers, with some patients achieving long-term remission without clinical recurrence. However, only a minority colon cancer respond to therapy. Here, we report protease-cleavable anti-PD-L1 antibody liposome, eLipo anti-PD-L1, for enhancing In vivo, is cleaved by legumain at site into pegylated and cancer-homing doxorubicin liposome. Functional...
Background and Aims: At present, there is a lack of simple reliable model for early prediction the efficacy etanercept in treatment juvenile idiopathic arthritis (JIA). This study aimed to generate validate models patients with JIA before administration using machine learning algorithms based on electronic medical record (EMR). Materials Methods: EMR data 87 treated between January 2011 December 2018 were collected retrospectively. The response was evaluated by DAS44/ESR-3 simplified...
Gene therapy offers potentially transformative strategies for major human diseases. However, one of the key challenges in gene is developing an effective strategy that could deliver genes into specific tissue. Here, we report a novel virus-like nanoparticle, bioorthgonal engineered recombinant biosome (reBiosome), efficient therapies cancer and inflammatory The mutant (mBiosome) first prepared by site-specific codon mutation displaying 4-azido-L-phenylalanine on vesicular stomatitis virus...
Abstract: In vivo evaluation of drug delivery vectors is essential for clinical translation. BALB/c nude mice bearing human breast cancer tumors, we investigated the biocompatibility, pharmacokinetics, and pharmacodynamics doxorubicin (DOX)-loaded novel cell-penetrating peptide (CPP)-modified pH-sensitive liposomes (CPPL) (referred to as CPPL(DOX)) with an optimal CPP density 4%. CPPL, a polyethylene glycol (PEG) derivative formed by conjugating PEG stearate via acid-degradable hydrazone...
Parkinson's disease (PD) is a leading cause of disability. Long noncoding RNA (LncRNA) OIP5-AS1 alleviates the accumulation and toxicity 1-methyl-4-phenylpyridine (MPP+ )/-induced α-synuclein in human neuroblastoma SH-SY5Y cells, which may be involved pathological process PD. This study explored neuroprotective effect lncRNA on MPP+ /-induced cell model PD, so as to provide theoretical basis for PD treatment. The was established group). overexpression vector oe-OIP5-AS1 constructed...
Overexpression of the components Janus kinase/signal transducer and activator transcription (JAK/STAT) signaling pathway are key factors pathogenic mechanisms underlying systematic juvenile idiopathic arthritis (SJIA). The present study aimed to investigate association between microRNA (miR)-19a, miR-21 JAK/STAT pathway. A total 20 patients with SJIA were included in study, peripheral blood mononuclear cells (PBMCs) from normal controls also collected. RNAiso was used extract RNA, RNA then...
We report the design, synthesis, and evaluation of a series novel cyclopentadienyl tricarbonyl (99m)Tc complexes as potent σ1 receptor radioligands. Rhenium compounds 3-(4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl)propylcarbonylcyclopentadienyl rhenium (10a) 4-(4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl)butylcarbonylcyclopentadienyl (10b) possessed high in vitro affinity for receptors moderate to selectivity σ2 vesicular acetylcholine transporter. Biodistribution studies mice demonstrated...
Clustered regularly interspaced short palindromic repeats (CRISPR) technology emerges a remarkable potential for cure of refractory cancer like metastatic breast cancer. However, how to efficiently deliver the CRISPR system with non-viral carrier remains major issue be solved. Here, we report kind targeted core-shell nanoparticles (NPs) carrying dual plasmids (pHR-pCas9) precise CCCTC-binding factor (CTCF) gene insert circumvent The NPs pHR-pCas9 can accomplish γGTP-mediated cellular uptake...