A5100644635- Radiopharmaceutical Chemistry and Applications
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- Medical Imaging Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- HER2/EGFR in Cancer Research
- Medical Imaging and Pathology Studies
- Nanoparticle-Based Drug Delivery
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Treatments and Mutations
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Prostate Cancer Treatment and Research
- Graphene and Nanomaterials Applications
- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Lung Cancer Diagnosis and Treatment
- Glioma Diagnosis and Treatment
- Intelligent Tutoring Systems and Adaptive Learning
- Caveolin-1 and cellular processes
- Cancer Cells and Metastasis
Peking University
2015-2025
Peking University International Hospital
2024
Henan Academy of Sciences
2024
Qiqihar University
2023
Haier Group (China)
2022-2023
Tongji University
2022
Institute of Acoustics
2022
Northeast Normal University
2009-2021
Beihua University
2021
Shanghai Jiao Tong University
2021
Effective cancer therapy depends not only on destroying the primary tumor but also conditioning host immune system to recognize and eliminate residual cells prevent metastasis. In this study, a integrin αvβ6-targeting peptide (the HK peptide)-functionalized graphene oxide (GO) was coated with photosensitizer (HPPH). The resulting GO conjugate, GO(HPPH)-PEG-HK, investigated whether it could destroy tumors boost antitumor immunity. We found that GO(HPPH)-PEG-HK exhibited significantly higher...
This report describes the synthesis of two cyclic RGD (Arg-Gly-Asp) conjugates, HYNIC-2PEG4-dimer (HYNIC = 6-hydrazinonicotinyl; 2PEG4-dimer E[PEG4-c(RGDfK)]2; and PEG4 15-amino-4,7,10,13-tetraoxapentadecanoic acid) HYNIC-3PEG4-dimer (3PEG4-dimer PEG4-E[PEG4-c(RGDfK)]2), evaluation their 99mTc complexes [99mTc(HYNIC-2PEG4-dimer)(tricine)(TPPTS)] (99mTc-2PEG4-dimer: TPPTS trisodium triphenylphosphine-3,3′,3′′-trisulfonate) [99mTc(HYNIC-3PEG4-dimer)(tricine)(TPPTS)] (99mTc-3PEG4-dimer) as...
<sup>99m</sup>Tc-3PRGD2 is a new SPECT tracer targeting integrin α<sub>V</sub>β<sub>3</sub> receptor for detecting tumors, imaging angiogenesis, and evaluating tumor response to therapy. A multicenter study was designed investigate the efficacy of evaluation patients with lung cancer. <b>Methods:</b> Seventy (51 men, 19 women; mean age ± SD, 63 9 y) suspected lesion whom definite pathologic diagnosis finally obtained (malignant, <i>n</i> = 58; benign, 12) were recruited from 6 centers....
Tumor relapse after initial regression post-chemotherapy is a major challenge in cancer treatment, as it usually leads to local-regional recurrence or inoperable distant metastasis.M2 macrophages diminish the tumor-inhibitory effect of chemotherapy and correlate with metastasis poor prognosis.In this study, we investigated whether molecular imaging M2 could serve an early biomarker for tumor lymph node preclinical mouse models.Methods: We developed macrophage-targeted probes near-infrared...
Integrin αvβ3 plays a critical role in tumor angiogenesis and metastasis. Radiolabeled RGD peptides that are integrin αvβ3-specific very useful for noninvasive imaging of expression rapidly growing metastatic tumors. In this study, we determined the binding affinity E{E[c(RGDfK)]2}2 (tetramer) its 6-hydrazinonicotinamide conjugate (HYNIC-tetramer) against 125I-echistatin to αvβ3-positive MDA-MB-435 breast cancer cells. The athymic nude mice bearing xenografts were used evaluate potential...
Significant evidence has indicated that tumor-associated macrophages (TAMs) play a critical role in the proliferation, invasion, angiogenesis, and metastasis of variety human carcinomas.In this study, we investigated whether near-infrared fluorescence (NIRF) imaging using macrophage mannose receptor (MMR; CD206)-targeting agent could be used to noninvasively visualize quantify changes TAMs vivo.The CD206-targeting NIRF agent, Dye-anti-CD206, was prepared characterized vitro vivo.By imaging,...
Overexpression of human epidermal growth factor receptor 2 (HER2) plays important roles in tumorigenesis and tumor progression breast cancer. Nuclear imaging HER2 expression tumors might detect all HER2-positive throughout the body guide HER2-targeted therapies for patients. We therefore aimed to develop a peptide probe cancer imaging. A novel SPECT probe, <sup>99m</sup>Tc-HYNIC-H6F, was prepared then evaluated animal models. <b>Methods:</b> The H6F (YLFFVFER) conjugated with bifunctional...
<sup>68</sup>Ga-labeled nanobody (<sup>68</sup>Ga-NC-BCH) is a single-domain antibody–based PET imaging agent. We conducted first-in-humans study of <sup>68</sup>Ga-NC-BCH for to determine its in vivo biodistribution, metabolism, radiation dosimetry, safety, and potential quantifying claudin-18 isoform 2 (CLDN18.2) expression in gastrointestinal cancer patients. <b>Methods:</b> Initially, we synthesized the probe performed preclinical evaluations on human gastric adenocarcinoma cell...
The expression level of PD-L1 in tumor tissue is considered one the effective biomarkers to guide PD-1/PD-L1 therapy. Quantifying whole-body by SPECT imaging may help selecting patients that potentially respond Nanobody smallest antibody fragment with antigen-binding ability well suited for radionuclide imaging. Nevertheless, high retention radioactivity kidney limit its clinical translation. present study aimed screen, design, and prepare a nanobody-based probe rapid renal clearance...
A novel dual-modality molecular probe composed of biocompatible Fe(3)O(4) nanocrystal, monoclonal antibody and radionuclide was designed prepared. All functional components in the probe, i.e., Fe(3)O(4), PEG, mAb 3H11 (125)I, were chemically bonded together for forming a stable probe. Systematic vitro experiments carried out evaluating biological activity targeting series vivo performed based on imaging detecting xenografted tumors nude mice by MRI gamma-imaging techniques. The...
Abstract Purpose: Abegrin is a monoclonal antibody to human integrin αVβ3, cell adhesion molecule highly expressed on actively angiogenic endothelium and glioblastoma multiforme tumor cells. The purpose of this study was evaluate the efficacy novel 90Y-Abegrin radioimmunotherapeutic agent in murine xenograft models with noninvasive vivo molecular imaging modalities. Experimental Design: A s.c. U87MG model used determine maximum tolerated dose (MTD), biodistribution, response, 90Y-Abegrin....
We recently designed and synthesized a Glu-c(RGDyK)-bombesin (RGD-BBN) heterodimeric peptide exhibiting dual integrin α(v)β(3) gastrin-releasing receptor (GRPR) targeting property. In this study, we investigated whether (99m)Tc-labeled RGD-BBN could be used for the noninvasive detection of lung carcinoma by using small-animal single-photon emission computed tomography (SPECT)/CT. was conjugated with 6-hydrazinonicotinyl (HYNIC) then radiolabeled (99m)Tc tricine TPPTS as coligands (TPPTS =...
Objectives Strategies to improve the responsiveness of programmed death-1 (PD-1)/programmed death ligand-1 (PD-L1) checkpoint blockade therapy remain an essential topic in cancer immunotherapy. In this study, we developed a new radiolabeled nanobody-based imaging probe 99m Tc-MY1523 targeting PD-L1 for enhanced therapeutic efficacy immunotherapy by guidance SPECT/CT imaging. Methods The binding affinity and specificity nanobody MY1523 were measured vitro. was with Tc site-specific...
Rationale:The decreased HER2-accessibility by epitope masking is a primary trastuzumab-resistance mechanism.In this study, we developed HER2-targeted dual radiotracer approach to predict the HER2-trastuzumab engagement noninvasively.Methods: Two novel HER2-specific VHHs, MIRC208 and MIRC213, were acquired immunizing alpaca with human HER2 protein, site-specifically labeled 99m Tc.Biodistribution SPECT/CT imaging studies performed in mice bearing HER2-positive HER2-negative tumors.The binding...
Fibroblast activation protein (FAP) is an emerging target for cancer diagnosis. Different types of FAP inhibitor (FAPI)-based radiotracers have been developed and applied tumor imaging. However, few FAPI tracers single photon emission computed tomography (SPECT) imaging reported. SPECT less expensive more widely distributed than positron (PET), thus, 99mTc-labeled FAPIs would be available to patients in developing regions. Herein, we a FAPI-04-derived radiotracer, HYNIC-FAPi-04 (HFAPi),...
Immunohistochemistry (IHC) for PD-L1 detection is limited by its invasiveness and heterogeneity of tumors. To address these challenges, a new PD-L1-targeted nanobody-based immune-PET radiotracer [18F]AlF-APN09 was developed using the site-specific radiolabeling method with complexing agent (Mal-RESCA) under mild conditions. prepared at room temperature (pH 4.6-4.8) within 20 minutes satisfactory radiochemical yields (45.8 ± 4.48%, non-decay corrected), high purity (>98%) moderate apparent...