A5023005054- Spine and Intervertebral Disc Pathology
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Bacterial Genetics and Biotechnology
- Cancer Immunotherapy and Biomarkers
- DNA Repair Mechanisms
- Osteoarthritis Treatment and Mechanisms
- Radiopharmaceutical Chemistry and Applications
- Cancer-related molecular mechanisms research
- Orthopedic Infections and Treatments
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Musculoskeletal pain and rehabilitation
- Mesenchymal stem cell research
- Cervical and Thoracic Myelopathy
- Infectious Diseases and Tuberculosis
- RNA and protein synthesis mechanisms
- Anatomy and Medical Technology
- Extracellular vesicles in disease
- Orthopaedic implants and arthroplasty
- Neurogenesis and neuroplasticity mechanisms
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Colorectal Cancer Surgical Treatments
- Cancer therapeutics and mechanisms
Army Medical University
2004-2025
Peking University
2022-2024
Peking University Cancer Hospital
2022-2024
Fuyang City People's Hospital
2023-2024
Ministry of Education of the People's Republic of China
2024
National Medical Products Administration
2023-2024
Inner Mongolia University
2015-2023
Inner Mongolia People's Hospital
2018-2022
Huazhong University of Science and Technology
2020
Wuhan Puai Hospital
2020
Acetamiprid (ACE) and imidacloprid (IMI) are two major members in the family of neonicotinoid pesticides, which synthesized with a higher selectivity to insects. The present study determined compared vitro effects ACE, IMI nicotine on mammalian reproduction by using an integrated testing strategy for reproductive toxicology, covered sperm quality, penetration into oocytes preimplantation embryonic development. Direct chemical exposure (500 µM or 5 mM) spermatozoa during capacitation was...
Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age, and oocyte developmental competence altered patients with PCOS. In recent years microRNAs (miRNAs) have emerged as important regulators gene expression, the aim study was to miRNAs expression patterns cumulus cells from PCOS patients. The included 20 undergoing vitro fertilization (IVF) intra-cytoplasmic sperm injection (ICSI): 10 diagnosed matching controls. We used deep sequencing technology...
Basal generation of reactive oxygen species (ROS) was essential for male reproductive function, whereas high ROS levels may be linked to low quality sperm and infertility. We examined the associations between in whole ejaculates among 1092 factor infertility (MFI) patients 50 donors with normal semen characteristics. were significantly positively correlated abnormal morphology rate, head defect, deformity index. Further, we investigated whether seminal plasma from MFI affects motility After...
The accumulated data suggest that inflammation can increase the level of reactive oxygen species (ROS), which contribute to impaired sperm function and male infertility. Therefore, we propose inflammation-mediated production ROS in female reproductive tracts hinder fertilisation. To test this hypothesis, phorbol myristate acetate (PMA) with polymorphonuclear leucocytes (PMNs) was applied generate endogenous ROS. We evaluated time-dependent effects on human motility, viability mitochondrial...
ABSTRACT Cardiac hypertrophy, a maladaptive response to chronic stress, progresses heart failure through mechanisms requiring deeper exploration. While forkhead helix transcription factor P3 (FoxP3) is well‐known as key regulator in CD4 + T cells, its role cardiomyocytes remains unclear. Here, by using isoproterenol (ISO)‐induced cardiac hypertrophy models (40 mg/kg daily for vivo study and 10 μmol/L vitro study), we revealed the protective of FoxP3 hypertrophy. Though modulating expression...
The sperm acrosome reaction takes place in response to progesterone and zona pellucida. Progesterone may act on more than one type of surface receptor, which is a gamma-aminobutyric acid (GABA) A-like receptor. Although there direct evidence GABA initiation mouse reaction, are conflicting results regarding GABA-induced exocytosis human spermatozoa. We have examined whether would initiate spermatozoa using the chlortetracycline assay zona-free hamster oocyte test. Human preincubated for > or...
Stem cell transplantation has enormous potential for the treatment of neurodegenerative disorders like Parkinson's disease (PD). Mesenchymal stem cells (MSCs) have attracted much attention because they can secrete a wide variety cellular factors that promote growth. In this study, we prepared conditioned medium (CM) using lyophilized MSC culture contained secretome MSCs and applied CM to neural (CM-NSCs) PD model rats. Quantitative real-time PCR, Western blot, immunocytochemistry were used...
Abstract Background The degenerative disc disease (DDD) is a major cause of low back pain. physiological low-glucose microenvironment the cartilage endplate (CEP) disrupted in DDD. Glucose influences protein O -GlcNAcylation via hexosamine biosynthetic pathway (HBP), which key to stem cell fate. Thiamet-G an inhibitor -GlcNAcase for accumulating -GlcNAcylated proteins while 6-diazo-5-oxo- l -norleucine (DON) inhibits HBP. Mechanisms DDD are incompletely understood but include CEP...
Real-time monitoring of the biological behavior extracellular vesicles (EVs) in vivo is limited, which hinders its application biomedicine and clinical translation. A noninvasive imaging strategy could provide us with useful information on EVs' distribution, accumulation homing vivo, pharmacokinetics. In this study, long half-life radionuclide iodine-124 (124I) was used to directly label umbilical cord mesenchymal stem cell-derived EVs. The resulting probe, namely, 124I-MSC-EVs, manufactured...
The chemoresistance and systemic toxicity of cisplatin (CDDP) severely limit its application in the treatment non-small cell lung cancer (NSCLC). Here, I-124 labeled membrane biomimetic nanovesicles loading Polyphyllin VI (PPVI) CDDP (termed 124I–P/C@CMLvs) were constructed to enhance sensitivity efficacy CDDP. radiochemical purity (RCP) 124I–P/C@CMLvs reached more than 99% maintained reliable stability vitro. Micro-positron emission tomography (micro-PET) imaging quantitatively revealed...
The present study was undertaken to determine the reproductive hazards of Di-(2-ethylhexyl)-phthalate (DEHP) on mouse spermatozoa and embryos in vitro genomic changes vivo. Direct low-level DEHP exposure (1 μg/ml) investigated by fertilization (IVF) process, culture preimplanted DEHP-supplemented medium embryo transfer achieve full term development. Big Blue® transgenic model employed evaluate mutagenesis testicular genome with vivo concentration (500 mg/kg/day). Generally, DEHP-treated...
Abstract The mechanical environment is crucial for intervertebral disc degeneration (IDD). However, the mechanisms underlying regulation of cartilage endplate (CEP) calcification by altered matrix stiffness remain unclear. In this study, we found that CEP was positively correlated with degree IDD and stiff matrix, which mimicked severe CEP, promoted inorganic phosphate-induced in chondrocytes. Co-expression analysis miRNA mRNA profiles showed increasing resulted up-regulation miR-20a...
Abstract Coating on the sperm surface, glycocalyx, plays a key role in motility, maturation and fertilization. A comprehensive profile of surface glycans will greatly facilitate both basic researches clinical studies. Because capability recognizing different glycan moieties, lectins are widely used glycobiology. However, lacking high-throughput technology, limited have been reported for analyzing human sperm. In this study, we employed lectin microarray profiling sperm, which 54 out 91...
In this study, we examined whether smoking and drinking affect sperm quality the DNA methylation of repetitive element LINE-1, MEST, P16, H19, GNAS in sperm. Semen samples were obtained from 143 male residents a minority-inhabited district Guizhou province southwest China. Quantitative analysis was performed using MassARRAY EpiTYPER assays. Sperm motility significantly lower both nicotine-exposed (P = 0.0064) nicotine- alcohol-exposed 0.0008) groups. Follicle-stimulating hormone (FSH) levels...