Temporal transcriptions of genes are achieved by different mechanisms such as dynamic interaction activator and repressor proteins with promoters, accumulation and/or degradation key regulators a function cell cycle. We find that the TorR protein localizes to old poles Escherichia coli cells, forming functional focus. The focus co-localizes nucleoid in cell-cycle-dependent manner, consequently regulates transcription number genes. Formation one at cells requires MreB DnaK proteins, ATP,...

To seek evidence of a primitive adaptive immune system (AIS) before vertebrate, we examined whether lymphocytes or lymphocyte-like cells and the related molecules participating in lymphocyte function existed amphioxus. Anatomical analysis by electron microscopy revealed presence gills, these underwent morphological changes response to microbial pathogens that are reminiscent those mammalian executing challenge. In addition, systematic comparative our cDNA database amphioxus identified large...

Adenanthin, a natural diterpenoid isolated from the leaves of Isodon adenanthus, has recently been reported to induce leukemic cell differentiation by targeting peroxiredoxins (Prx) I and II. On other hand, increasing lines evidence propose that these Prx proteins would become potential targets screen drugs for prevention treatment solid tumors. Therefore, it is significance explore activities adenanthin on tumor cells. Here, we demonstrate protein essential survival hepatocellular carcinoma...

Actin stress fibers are fundamental components of the actin cytoskeleton that produce contractile force in non-muscle cells. The formation is controlled by small GTPase RhoA and two highly related proteins, RhoB RhoC. Together, this subgroup actin-regulatory proteins represents canonical pathway stress-fiber formation. Here, we show Rif an alternative trigger epithelial distantly to RhoA; however, share a common downstream partner formation--the Diaphanous-related formin mDia1. Rif-induced...

Background The fast-growing bacterial cell cycle consists of at least two independent cycles chromosome replication and division. To ensure proper viability, division must be coordinated. It has been suggested that metabolism could affect the Escherichia coli cycle, but idea is still lacking solid evidences. Methodology/Principle Findings We found absence AspC, an aminotransferase catalyzes synthesis aspartate, led to generation small cells with less origins slow growth. In contrast, excess...

The uvrB gene belongs to the SOS network, encoding a key component of nucleotide excision repair. promoter region contains three identified promoters with four LexA binding sites, one consensus and six potential DnaA sites. More than 3-fold increase in transcription chromosomal is observed both ∆lexA ∆sulA cells dnaAA345S cells, 5-fold relative wild-type shown. full activity requires uvrBp1-2 uvrBp3 repressed by proteins. binds tightly LexA-box1 at irrespective presence this important for...

Abstract Transcription attenuation in response to the availability of a specific amino acid is believed be controlled by alternative configurations RNA secondary structures that lead arrest translation or release arrested ribosome from leader mRNA molecule. In this study, we first report possible example DnaA‐dependent riboswitch for transcription Escherichia coli . We show (i) DnaA regulates structural genes but not hisL gene; (ii) might bind rDnaA boxes present HisL‐SL RNA, and...

Piper longum L. is a well-known traditional antihyperlipidemic medicine in China, containing medicinal constituents of piperine, pipernonaline and piperlonguminine its fruit. However, the antitumor properties these have not yet been studied. We found that potassium piperate (GBK), derivative inhibited proliferation cancer cells. GBK selectively G1-S-phase transition breast cells G1 arrest was correlated with induction p27 expression, which an inhibitor for cyclin-dependent kinases,...

Abstract Multiparametric image analysis is highly preferable in revealing complicated biological processes. The multiplexing capability demanding and limited by spectral overlap of fluorescent dyes the number channels available an imaging platform, especially when both nuclear cytoplasmic staining are required for analysis. Here, we introduce a retrospective method that enables cell labeling restaining cells with sulforhodamine B (SRB) using positioning‐stage equipped microscope automated...

Members of the well-known semaphorin family proteins can induce both repulsive and attractive signaling in neural network formation their cytoskeletal effects are mediated part by small guanosine 5'-triphosphatase (GTPases). The aim this study was to investigate cellular role Rif GTPase neurotrophin-induced neurite outgrowth. By using PC12 cells which known cease dividing begin show outgrowth responding nerve growth factor (NGF), we found that 6A as effective at stimulating cells, its...

The trans-translation pathway, mediated by the transfer messenger RNA (tmRNA; encoded ssrA gene) and SmpB protein (tmRNA-binding expressed in Salmonella enterica), which is conserved bacteria, required for various cellular processes.A previous study has shown that to ensure timely (non-delayed) dnaA transcription consequent initiation of DNA replication Caulobacter crescentus.In this study, we observed chromosome was delayed Escherichia coli lacking smpB and/or genes (DssrA, DsmpB, or...

The MiniR1–1 plasmid is a derivative of the R1 plasmid, low copy cloning vector. Nucleotide sequencing analysis shows that 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication). carries repA, tap, copA and bla genes, genes ORF1 ORF2. contains eight DnaA-binding sites (DnaA-boxes). DnaA-box1 in region fully matched to DnaA-box consensus sequence, DnaA-box8, one mismatch, close gene. presence leads accumulation D-period cells increase cell size slowly growing...

Abstract Histone‐like nucleoid‐structuring protein (H‐NS) and integration host factor (IHF) are major nucleoid‐associated proteins, DnaA, a replication initiator, may also be related with nucleoid compaction. It has been shown that protein‐dependent DNA compaction is many aspects of bacterial physiology, including transcription, replication, site‐specific recombination. However, the mechanism physiology resulting from remains unknown. Here, we show H‐NS important for correct in...

Lysophosphatidic acid (LPA) and geminin are overexpressed in ovarian cancer, increasing evidence supports their contribution to tumor development. Here, we reveal that depletion induces autophagy suppression enhances reactive oxygen species (ROS) production apoptosis of high-grade serous cancer (HGSOC) cells. Bioinformatics analysis pharmacological inhibition studies confirm LPA activates expression the early S phase HGSOC cells

Geminin, an inhibitor of the DNA replication licensing factor, chromatin and factor (Cdt) 1, is essential for maintenance genomic integrity. As a multifunctional protein, geminin also involved in tumor progression, but molecular details are largely unknown. Here, we found that lysophosphatidic acid (LPA)–induced upregulation was specific to gastric cancer cells. LPA acted via receptor (LPAR) 3 matrix metalloproteinases (MMPs) signaling transactivate epidermal growth (EGFR) (Y1173) thereby...

Breast cancer is the second cause of death in women, and tumor metastasis primary mortality. Due to involvement many regulatory molecules signaling pathways, occurrence development metastases needs be further studied. MicroRNAs (miRNAs) are ubiquitously expressed small non-coding RNAs that have been shown play an important role diagnosis treatment diseases, as well representing attractive candidate for control. In this study, we investigated mechanism potassium piperonate (GBK) impairing...

Abstract The Escherichia coli YfiF protein is functionally unknown, being predicted as a transfer RNA/ribosomal RNA (tRNA/rRNA) methyltransferase. We find that absence of the yfiF gene delays initiation chromosome replication and delay reversed by ectopic expression YfiF, whereas excess causes an early initiation. A slight decrease in both cell size number origin per mass observed Δ cells. does not genetically interact with proteins such DnaA, DnaB, DnaC. Interestingly, associated ribosome...