A5085370574- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Plant and Fungal Interactions Research
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- RNA Research and Splicing
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Animal Virus Infections Studies
- Cancer-related Molecular Pathways
- Plant Pathogens and Resistance
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Nerve injury and regeneration
University of Pittsburgh
2016-2025
Yale University
2023
Stony Brook University
2010
Agricultural Research Organization
1995-2009
Tel Aviv University
1999-2008
Rabin Medical Center
1999-2008
The University of Texas Southwestern Medical Center
2007
Pittsburg State University
2006
Ministry of Agriculture and Rural Development
2000
Children's Hospital of Pittsburgh
1996-1997
Glioblastoma (GB) remains the most aggressive primary brain malignancy. Adoptive transfer of chimeric antigen receptor (CAR)-modified immune cells has emerged as a promising anti-cancer approach, yet potential utility CAR-engineered natural killer (NK) to treat GB not been explored. Tumors from approximately 50% patients express wild-type EGFR (wtEGFR) and in fewer cases both wtEGFR mutant form EGFRvIII; however, previously reported CAR T cell studies only focus on targeting EGFRvIII. Here...
The activation of the cellular oncogene c-mos in mouse plasmacytoma XRPC24 was found to result from insertion a 4.7-kilobase-pair DNA element, within coding region. element terminates on both sides with direct repeat around 335 nucleotides. as well internal sequences show strong homology endogenous intracisternal A-particle (IAP) genes. IAP genome integrated head-to-head (5' 5') configuration. This juxtapositioned 5' long terminal next bulk oncogene's region and shifted flanking position...
Glioblastoma multiforme (GBM) remains an untreatable human brain malignancy. Despite promising preclinical studies using oncolytic herpes simplex virus (oHSV) vectors, efficacy in patients has been limited by inefficient replication tumor cells. This disappointing outcome can be attributed part to attenuating mutations engineered into these viruses prevent normal Alternatively, retargeting of fully replication-competent HSV tumor-associated receptors the potential achieve specificity without...
We examined by Southern blotting the state of cellular oncogene c-myc in dog transmissible venereal tumor. The tumor DNA contains a 16.8-kilobase pair (kbp) rearranged fragment addition to normal 15-kbp and 7.5-kbp fragments. compared structure cloned (re-myc) with that found rearrangement was due insertion 1.8-kbp upstream first exon c-myc. inserted is flanked 10-base-pair direct repeats dA-rich tail, suggesting its origin from mRNA. Partial sequence element showed 62% homology primate...
The organization and evolution of immunoglobulin variable region genes was studied by comparing human mouse heavy chain (VH) genes. We show that a VH gene subgroup constitutes physically linked multigene family separated from another subgroup. mapped the VHIII to be 3' VHII based on deletion after V-D-J rearrangement. results indicate underwent significant expansion as compared Amino acid sequence data correspond only small subset Human contain shorter intron are two codons than most BALB/c...
Five closely related immunoglobulin VH genes (subgroup II) were compared by sequencing of several kb DNA. In three the homology greater than 75% was found along an area 4 that includes coding region. The in flanking regions is only slightly lower sequences. Two other genes, which are located on same EcoRI fragment, show high to first and immediately regions. more distant no with genes. This indicates their evolutionary history differs from A region simple DNA sequence composed repetitive TCC...
Glioblastoma multiforme (GBM) is an aggressive brain cancer for which there no effective treatment. Oncolytic HSV vectors (oHSVs) are attenuated lytic viruses that have shown promise in the treatment of human GBM models animals, but their efficacy early phase patient trials has been limited. Instead attenuating virus with mutations virulence genes, we engineered four copies recognition sequence miR-124 into 3'UTR essential ICP4 gene to protect healthy tissue against replication; expressed...
Significance Gene therapy has made significant strides in the treatment and even cure of single-gene defects. However, maturation this field will require more sophisticated vehicles capable cell-selective delivery large genetic payloads whose regulated expression restore or enhance cellular functionality. High-capacity herpes simplex virus vectors have potential to meet these challenges but been limited by need preserve one particularly cytotoxic viral product, infected cell polypeptide 0,...
<h2>Abstract</h2> Experiments were carried out in a nude mouse model of human glioblastoma to determine whether gamma-knife radiosurgery combined with herpes simplex virus thymidine kinase (tk) suicide gene therapy and tumor necrosis factor alpha (TNFα) transfer provided an improved multimodality treatment this disease. Animals inoculated intracerebrally 2 × 10<sup>5</sup> U-87MG cells establish brain tumors. At 3 days postinoculation, the region was injected 10<sup>6</sup> infectious...
Tumor cell transduction with the herpes simplex virus (HSV) thymidine kinase (tk) gene and treatment ganciclovir (GCV) is a widely studied cancer therapy. Connexin (Cx)-dependent gap junctions between cells facilitate intercellular spread of TK-activated GCV, thereby creating bystander effect that improves tumor killing. However, often have reduced connexin expression, thus thwarting killing effectiveness TK/GCV To improve this therapy, we compared an HSV vector (TOCX) expressing Cx43 in...
Summary A virus causing sunken veins on ‘Georgia Jet’ sweet potato, and yellow brittle leaves stunting Ipomoea setosa , was purified a specific antiserum prepared. Flexuous particles with normal length of 850 nm diameter 12 an open helical structure typical closteroviruses were observed. The particle protein has apparent mol. wt c. 34 kD. Double‐stranded RNA isolated from SPSVV‐infected I. subjected to electrophoresis in agarose consisted one major band estimated M r 10.5 kbp two minor bands...
ABSTRACT Equine herpesvirus type 1 (EHV-1), a member of the Alphaherpesviridae , displays broad host range in vitro, allowing for detailed study mechanisms productive infection, including attachment and entry, various cell culture systems. Previously, we showed that EHV-1 infects Chinese hamster ovary (CHO-K1) cells even though these do not express known alphaherpesvirus entry receptor. In this report, show by electron microscopy an infectious recovery assay into CHO-K1 occurs via endocytic...
ABSTRACT Herpes simplex virus (HSV) entry into cells is triggered by the binding of envelope glycoprotein D (gD) to a specific receptor, such as nectin-1 or herpesvirus mediator (HVEM), resulting in activation fusion effectors gB and gH penetration. Here we report identification hyperactive allele, D285N/A549T, selected repeat passage gD mutant defective for through that express gD-binding-impaired nectin-1. The allele wild-type background enabled use other nectins receptors. In addition,...
Multiple studies have indicated that in addition to direct oncolysis, virotherapy promotes an antitumor cytotoxic T cell response important for efficacy. To study this phenomenon further, we tested three syngeneic murine sarcoma models displayed varied degrees of permissiveness oncolytic herpes simplex virus replication and cytotoxicity vitro, with the most permissive being comparable some human tumor lines. The vivo effect ranged from no or modest complete regression protection rechallenge....