Head and Neck Squamous Cell Carcinoma (HNSCC) tumors are often resistant to therapies. Therefore searching for predictive markers new targets treatment in clinically relevant vitro tumor models is essential. Five HNSCC-derived cell lines were used assess the effect of 3D culturing compared 2D monolayers terms proliferation, response anti-cancer therapy as well expression EMT CSC genes. The viability proliferation capacity HNSCC cells induction apoptosis spheroids after was assessed by MTT...

A growing body of evidence suggests that components the tumor microenvironment, including cancer-associated fibroblasts (CAF), may modulate treatment sensitivity cells. Here, we investigated possible influence CAFs on head and neck squamous cell carcinoma (HNSCC) lines to cetuximab, an antagonistic epidermal growth factor receptor (EGFR) antibody. Cetuximab caused a reduction in proliferation rate HNSCC lines, whereas HNSCC-derived CAF cultures was unaffected. When cells were cocultured with...

Bcl-2 family members have long been known to control permeabilization of the mitochondrial membrane during apoptosis, but involvement these proteins in lysosomal (LMP) was not considered until recently. The aim this study investigate mechanism underlying release proteases cytosol seen with special emphasis on role Bax. In human fibroblasts, exposed apoptosis-inducing drug staurosporine (STS), protease cathepsin D observed by immunocytochemistry. response STS treatment, there a shift Bax...

The effects of hydrogen peroxide on cell viability and, in particular, lysosomal integrity were investigated a model system cultured, established, macrophage-like J-774 cells. cells found to rapidly degrade added peroxide, withstanding concentrations 250μM without death; however, all tested (100-500/μM) substantially decreased cellular ATP approximately the same degree. Concentrations 500/μM resulted pronounced and rapid decrease preceded by loss integrity, as judged relocalization acridine...

AbstractRadiotherapy remains the backbone of head and neck cancer therapy but response is sometimes impeded by tumor radioresistance. Identifying predictive biomarkers radiotherapy a crucial step towards personalized therapy. The aim this study was to explore gene expression data in search squamous cell carcinoma (HNSCC).Microarray analysis performed on five lines with various intrinsic radiosensitivity, selected from panel 29 HNSCC lines. bioinformatics approach included Gene Ontology (GO)...

Mortality in head and neck squamous cell carcinoma (HNSCC) is high due to emergence of therapy resistance which results local regional recurrences that may have their origin resistant cancer stem cells (CSCs) or with an epithelial-mesenchymal transition (EMT) phenotype. In the present study, we investigate possibility using surface expression CD44 epidermal growth factor receptor (EGFR), both been used as markers, identify subpopulations within HNSCC lines differ respect phenotype treatment...

Abstract Background Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors for which the overall survival rate worldwide around 60%. The tumor microenvironment, including cancer-associated fibroblasts (CAFs), believed to affect treatment response migration HNSCC. aim this study was create biologically relevant HNSCC in vitro model consisting both cells CAFs cultured 3D establish predictive biomarkers response, as well investigate impact on phenotype, proliferation...

Biogenesis of phagolysosomes proceeds through a sequential series interactions with endocytic organelles, process known to be regulated by Rab and SNARE proteins. The molecular mechanisms underlying phagosome maturation in neutrophils are, however, not clearly understood. We investigated fusion between phagosomes containing the intracellular pathogen Mycobacterium tuberculosis versus extracellular Staphylococcus aureus (designated MCP for mycobacteria-containing SCP S. aureus-containing...

Secondary lysosomes contain low-molecular weight iron-complexes as a consequence of normal autophagocytotic degradation various metallo-proteins. Thus, entry hydrogen peroxide into these organelles may induce ironcatalyzed oxidative reactions with ensuing damage to lysosomal membranes and leakage destructive contents. The amount reactive iron the cellular capacity degrade would then be important determining factors in resistance stress. effects on cell viability and, particular, membrane...

We previously identified WRAP53 as an antisense transcript that regulates the p53 tumor suppressor. The gene also encodes a protein essential for Cajal body formation and involved in cellular trafficking of survival motor neuron complex, telomerase enzyme small body-specific RNAs to bodies. Here, we show is overexpressed variety cancer cell lines different origin overexpression promotes transformation. Knockdown triggers massive apoptosis through mitochondrial pathway, demonstrated by...

Combination treatment (chemoradiotherapy) is the standard for locally advanced head and neck squamous cell carcinoma (HNSCC); however, resistance local recurrence are significant problems. A high level of epidermal growth factor receptor (EGFR) has been associated with a more aggressive phenotype as well decreased responsiveness to radio- or chemotherapy. We examined role EGFR status ligand expression response.Intrinsic sensitivity radiotherapy, cisplatin, cetuximab treatments was...

Head and neck squamous cell carcinoma (HNSCC) is a malignancy that associated with severe mortality despite advances in therapy. Today's standard treatment most commonly includes radiotherapy, often combined chemotherapy or surgery. There are so far no established biomarkers to predict response radiation, thus the aim of this study was investigate series markers could potentially identify HNSCC patients who would benefit from radiotherapy. The selected markers, both proteins (epidermal...

One of the hallmarks cancers is their ability to develop resistance against therapeutic agents. Therefore, developing effective in vitro strategies identify drug remains paramount importance for successful treatment. ways cancer cells achieve through expression efflux pumps that actively pump drugs out cells. To date, several studies have investigated potential using 3-dimensional (3D) multicellular tumor spheroids (MCSs) assess resistance; however, a unified system uses MCSs differentiate...

Background: The aim of this study was to evaluate biomarkers and biological characteristics tumor biopsies from patients with head neck cancer (HNC) assess the risk early death. Furthermore, we analyzed whether any combination markers could be used for prognostication death within six months after diagnosis. Materials Methods: Patients diagnosed HNC, receiving curative treatment decision at a multidisciplinary board meeting, who died diagnosis were included in study. Nine identified matched...

Previous studies have shown that a variety of mammalian cell types, including macrophages, contain small amounts redox-active iron in their lysosomes. Increases the level this pool predispose to oxidative stress. Limiting availability intralysosomal could therefore represent potential cytoprotection for cells under stress.In present study we an initial 6 h exposure J774 macrophages 30 μM iron, added culture medium as FeCl3, increased lysosomal content and sensitivity H2O2-induced (0.25 mM...