Abstract. Ibrahim A, Artama WT, Budisatria IGS, Yuniawan R, Atmoko BA, Widayanti R. 2021. Regression model analysis for prediction of body weight from measurements in female Batur sheep Banjarnegara District, Indonesia. Biodiversitas 22: 2723-2730. Bodyweight is an important aspect livestock management. The present study was undertaken to estimate correlation coefficients between biometric traits and identify best predictor measurements. Data on (body length: BL, chest girth: CG withers...

Background and Aim: Staphylococcus aureus is a significant foodborne pathogen responsible for producing enterotoxins, particularly staphylococcal enterotoxins A (SEA) B (SEB), which are frequently found in milk dairy products. These toxins raw pose risk to public health, necessitating accurate rapid detection methods. This study aimed develop evaluate two immunoassays – antigen capture enzyme-linked immunosorbent assay (AC-ELISA) dot-blot detecting SEA SEB cow milk. The sensitivity...

Background and Aim: The impact of exogenous melatonin on the sperm quality small ruminants is controversial. Therefore, this study aimed to synthesize previous findings influence injection quality, steroid hormones, testicular blood flow in ruminants. Materials Methods: Thirty studies were analyzed by computing raw mean difference (RMD) as effect size between control treatment groups, using inverse variance for random-effect model method moments DerSimonian Laird. We assessed heterogeneity...

The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep's genetic profile. This study aimed diversity and relationships several Indonesian local sheep populations on Java Island, Indonesia, based mtDNA Cyt sequences. Blood samples were collected from forty-one individual in seven Indonesia breeds Island (Priangan = 6, Garut Batur 7, Wonosobo 5, Javanese Thin-Tailed/JTT Fat-Tailed/JFT Sapudi 5). extraction was performed blood samples,...

Phylogenetic relationships and genetic variation among 9 Indonesian goat breeds were analyzed using cytochrome b gene sequences.The genomic DNA was isolated by gSYNC Mini Kit (Geneaid) amplified PCR method with a pair of primer.Complete sequences Cyt genes goats is 1140 bp contained 19 variable sites 8 haplotypes between C. hircus (D84201), when compared to aegagrus falconeri, there are 59 sites.Phylogenetic analysis haplotype in the combination from GenBank showed that grouped...

Eid al-Adha is one of the most important festivals celebrated by Muslims in Indonesia. Roadside livestock traders open their stalls during period. This study aimed to investigate characteristics and behaviors roadside urban peri-urban areas Yogyakarta, Indonesia.In-depth interviews with 36 were conducted on August 7-23, 2018 (n=20) (n=16) Yogyakarta. The collected data analyzed descriptive statistical analysis using one-way variance.The results indicate that trading activities last longer...

Aim: This study aimed to determine the genetic characterization and phylogenetic structure of Indonesian indigenous catfish using cytochrome B (Cyt B) sequences. Materials Methods: The genomes 26 catfishes caught from nine rivers different geographical locations around Indonesia were analyzed. tissue isolation method was used isolate total genome fishes. Furthermore, polymerase chain reaction done amplify mtDNA Cyt CytBF CytBR primers. Following sequencing, analysis variation relationship...

Java Island is one of the islands in Indonesia which has local sheep breeds with specific characteristics and native development geography certain regions. This study aimed to determine genetic profiles maternal origin six on Island.This was conducted by identifying complete mitochondrial DNA (mtDNA) displacement loop (D-loop) region sequences a total 22 individual Island, including Javanese thin-tailed (JTT), Fat-Tailed (JFT), Batur (BTR), Wonosobo (WSB), Garut (GRT), Priangan (PRG) sheep....

Indonesia has several breeds of goats as the source genetic.This study aimed to characterize genetic Indonesian goats.The phylogenetic relationship among and variability within eight native were analyzed using mtDNA D-loop region sequences.The results show that length complete sequences in are1212 bp.There are 68 polymorphic sites 21 different haplotypes.Haplotype diversity nucleotide based on 0.5000-1.000and 0.248-1.1595%,respectively.The value distance between groups ranged from...

Background: Hutan Sumatera goat (Sumatran serow) is one of wildlife that quite similar to the domesticatic goats and have never been investigated at molecular level.Indonesian society believes belongs group genus Capra, therefore, this animal called a wild or forest goat.Hutan has categorized as an endangered species in IUCN red list.Materials Methods: The genomic DNA Sumatran serow Indonesian were isolated by gSYNC Mini Kit (Geneaid) cytochrome b gene was amplified using PCR method with...

Cuscuses is marsupials animal (Phalangeridae) which has limited spread in eastern Indonesia (Sulawesi, Maluku, Papua and Timor islands), Australia New Guinea. The ex-situ in-situ conservation of cuscuses under captivating condition an alternative solution to protect from extinction. This study aimed determine nucleotide sequences genetic marker on cyt b gene with sequencing method each species two provinces. Whole genome DNA was extracted 22 samples obtained different habitats, Maluku (13...

Cuscuses is marsupials animal (Phalangeridae), which has limited spread in Eastern Indonesia (Sulawesi, Maluku, Papua and Timor islands), Australia New Guinea.This study, the phylogenetic relationship of cuscuses from Maluku based on mitochondrial sequences NADH dehydrogenase subunit 1 gene was investigated.Whole genome DNA extracted 22 tissue biopsy samples (13 individuals) (9 according to protocol Qiamp Blood Mini Kit (Qiagen) then it used as template for amplification ND1 by using PCR...

The Samosir goat has a high cultural value and is source of germplasm in Indonesia. This study aimed to reveal the history selection signatures goat. A total 25 goats were divided into seven subpopulations Indonesian breeds. Deoxyribonucleic acid (DNA) from blood samples was isolated with use gSYNC™ DNA Mini Kit (Geneaid, Taipei, Taiwan). Cytb gene amplification performed by polymerase chain reaction (PCR) method, PCR products sequenced. phylogenetic tree constructed neighbor-joining method...

Aim: This study aimed to analyze the genetic variation and phylogenetic reconstruction of Indonesian indigenous catfish using mitochondrial cytochrome oxidase subunit III sequences. Materials Methods: A total 19 samples were collected from seven rivers (Elo [EM], Progo [PM], Kampar [KR], Musi [MP], Mahakam [MS], Kapuas [KS], Bengawan Solo [BSBJ]) in five different geographical locations Indonesia. The genome was isolated tissue. Mitochondrial DNA amplified polymerase chain reaction (PCR)...

Abstract Sheep play an important role for Indonesian people, especially during Eid al-Adha celebration. There are several criteria in the selection of sheep as sacrificial animals. This study was aimed to observe consumer’s preferences attributes celebration based on information gathered from roadside traders. conducted by using in-depth interview and ranking 36 traders Yogyakarta, Indonesia. The data were analysed descriptively. results showed that body characteristics, weight, posture, age...

Aedes aegypti is the vector of dengue fever, hemorrhagic chikungunya, and, most recently, Zika. Dengue fever one Indonesia's endemic diseases. The principal tool for preventing controlling Ae. by chemical insecticides since vaccine against still under research. However, developed resistance to various worldwide. Therefore, research on alternate compounds as mosquito urgently needed. This study demonstrated efficacy Artemisia vulgaris extract larvicidal, ovicidal, adulticidal, repellency, and...

Background and Aim: Excessive use of trypanocidal drugs can lead to cases drug resistance. Multiple resistance have been widely reported for such as isometamidium chloride diminazene aceturate. These deserve serious attention, especially in Indonesia, where the first case was recorded molecular basis has never evaluated. This study aimed analyze multidrug protein (MRP) gene Trypanosoma evansi isolates, sampled from by focusing on phylogenetic relationship between these isolates other spp....

Aim: n-Propanol extracts from fresh, boiled, and fermented seeds were studied to evaluate their neuroprotective effects in a Parkinson's disease (PD) rat model, based on the total number of dopaminergic (DA) neurons substantia nigra pars compacta (SNpc).Materials Methods: Rats induced with paraquat dichloride at dosage 7 mg/kg BW intraperitoneally twice week same time supplemented extract 70 orally every day for 3 weeks.On 24 th day, all rats perfused fixed 4% paraformaldehyde.The left part...

Abstract Eid al-Adha is one of the important religious festivals for Muslims in world. Sheep traders can be divided into primary and opportunist based on trade activity this period. This study aims to investigate preferences sheep marketing place their satisfaction during period Yogyakarta, Indonesia. In-depth semi-structured interviews with 59 were conducted Juli 15 – August 25, 2018. The collected data analyzed by descriptive analysis (score, index rank). results indicate that most...

Abstract Molecular sex determination is an effective solution to determine because it can be done early in the growing phase of a bird and results are more accurate. carried out based on chromodomain helicase DNA binding (CHD) gene by using NP, P2, MP primers. The purpose this study was peach-faced lovebird ( Agapornis roseicollis ) detecting intron size CHD Z chromosome W polymerase chain reaction (PCR). samples were isolated from feathers 14 lovebirds belonging owners, which sent...

The study described the development of a haptoglobin-based diagnostic tool for mastitis in Ettawa crossbreed goats. Fifty eight milk samples were collected from flock goats Yogyakarta, Central Java, Indonesia. All tested using California Mastitis Test (CMT), Somatic Cell Count (SCC), and Polymerase Chain Reaction (PCR) to identify Staphylococcus aureus, Streptococcus uberis agalactiae. presence haptoglobin mRNA proteins somatic cells was detected Sanger sequencing SDS-PAGE, respectively....

Background and Aim: The availability of fertility markers is crucial for maintaining, protecting, improving the genetics Jawa-Brebes (Jabres) cows. Follicle-stimulating hormone receptor (FSHR) insulin-like growth factor-1 (IGF-1) play critical roles in female reproductive physiology. single-nucleotide polymorphisms (SNPs) FSHR G-278A> IGF-1 C-512T correlate with cows' traits. This study aimed to identify these SNPs their potential associations parameters Jabres Materials Methods: Samples...