A5004433939- Reproductive Biology and Fertility
- Livestock Farming and Management
- Sperm and Testicular Function
- Food and Agricultural Sciences
- Aquatic life and conservation
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Renal and related cancers
- Reproductive Health and Technologies
- Public Health and Nutrition
- Effects of Environmental Stressors on Livestock
- Tissue Engineering and Regenerative Medicine
- Plant tissue culture and regeneration
- Computer Science and Engineering
- Occupational Health and Safety Management
- Enzyme Production and Characterization
- Legal Studies and Policies
- Osteoarthritis Treatment and Mechanisms
- Shallot Cultivation and Analysis
- Methodologies in Health Research and Practice
- Livestock Management and Performance Improvement
- Genomics, phytochemicals, and oxidative stress
Universitas Gadjah Mada
1970-2024
American Society of Composers, Authors and Publishers
2015
Yamaguchi University
2003-2013
Institute of Livestock and Grassland Science
2010-2013
Research Institute for Farm Animal Biology (FBN)
2004
Kobe University
2004
The present study was conducted to investigate the effects of attachment cumulus cells porcine oocytes during process maturation and fertilization on nuclear maturation, subsequent development after in vitro (IVF). In first experiment, were removed from cumulus-oocyte complexes (COCs) at 0, 24 42 h onset culture then cultured until reaching cultivation. second COCs denuded as described fertilized for 7 days. As a control, allowed maintain end IVF. proportion metaphase II significantly...
Abstract Indonesia has the largest sago palm ( Metroxylon spp.) cultivation worldwide, producing starch as a significant carbohydrate source across various regions. A primary challenge in maintaining quality and safety of products is microbial contamination, which can occur from initial stages production. The production process, including washing, grating, drying, significantly impacts contamination starch. washing process materials used influence produced, while grating also plays critical...
The present study was conducted to investigate the effects of storage time and temperature porcine ovaries on quality nuclear maturation in vitro oocytes obtained from stored their subsequent development after fertilization. were physiological saline for 0, 3, 6, 9 12 h at various temperatures (4, 15, 25 35 C). pH follicular fluid ovaries, DNA fragmentation oocyte nucleus meiotic competence examined. Some C 6 fertilized vitro, then cultured 7 days examine ability embryos develop blastocyst...
Background and Aim: The impact of exogenous melatonin on the sperm quality small ruminants is controversial. Therefore, this study aimed to synthesize previous findings influence injection quality, steroid hormones, testicular blood flow in ruminants. Materials Methods: Thirty studies were analyzed by computing raw mean difference (RMD) as effect size between control treatment groups, using inverse variance for random-effect model method moments DerSimonian Laird. We assessed heterogeneity...
It has been suggested that the maturational stage of oocytes at time insemination influences sex ratio resulting embryos. However, there are very few reports concerning relationship between maturation culture period and The objective this study was to investigate effects in vitro for bovine on produced blastocysts using a novel technique loop-mediated isothermal amplification (LAMP). Cumulus-oocyte complexes were collected from ovaries slaughtered cows, then matured various periods (16, 22,...
Interspecies nuclear transfer (NT) could be an alternative to replicate animals when supply of recipient oocytes is limited or in vitro embryo production systems are incomplete. In the present study, embryonic development was assessed following interspecies NT donor cumulus cells derived from yak and dog into ooplasm domestic cow. The percentages fusion subsequent eight-cell stage embryos were comparable those intraspecies (cow-cow embryos). percentage blastocysts significantly lower (p <...
We conducted two experiments to investigate the effects of storage temperature and period for cat ovaries on meiotic developmental competence oocytes collected from ovaries. In Experiment 1, were stored in physiological saline 24 h at 4 C, 23-25 or 38 C (cold, room, incubator groups, respectively), then each group. Morphologically intact selected cultured maturation medium h. Significantly more reached metaphase II (MII) cold group (53.4%) than room groups (20.0 2.4%, respectively). 2, 0, 6,...
The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation survival and DNA damage after exposure cryoprotectants. Porcine blastocysts were exposed 10% ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees C) then cultured in vitro 24 h. rates PD GLY significantly lower than those control which carrier solution without Significantly more DNA-fragmented nuclei occurred cryoprotectant-exposed blastocysts,...
The present study was conducted to examine the relationship between oxygen consumption rate and sex ratio of bovine in vitro fertilized embryos on each day blastocyst formation. quality blastocysts collected 7, 8, 9 after fertilization (IVF) were categorized as ranks A B (excellent good, respectively) based microscopic observation morphology. individual evaluated using two novel techniques: scanning electrochemical microscopy (SECM) loop-mediated isothermal amplification (LAMP),...
Pemeliharaan sapi Bali di Kotamadya Bengkulu dengan sistem Sistem Integrasi Sapi - Kelapa Sawit (SISKA) sudah berjalan beberapa tahun. Salah satu parameter keberhasilan program ini adalah performa reproduksi Bali. Performa menggambarkan kualitas dari manajemen pemeliharaan yang telah dilakukan. Kajian tersebut dilakukan pemeriksaan secara per rektal dan analisa data recording peternak petugas. Tujuan utama mendapatkan produksi optimal keuntungan maksimal. Efisiensi menentukan produktivitas,...
The utilization of cassava leaves and peels, ceara rubber leaves, sweet potato Chinese Albizia lophatheri from Bojonegoro Regency has led to the poisoning livestock due antinutritional factors. Nevertheless, plants are known have bioactive components potential antioxidant antibacterial activity if appropriately processed. This study aimed determine compounds as well these responsible for in Regency.Extraction was performed by maceration method using 70% (v/v) ethanol solvent. samples were...
Abstract The present study was conducted to investigate effects of antioxidants during maturation culture recipient oocytes and/or gene‐transfected donor cells on the meiotic competence oocytes, and developmental quality reconstructed embryos after nuclear transfer (NT) in cattle. Gene‐transfected had negative proportions blastocyst formation, total cell numbers, DNA fragmentation indices embryos. Supplementation either vitamin E (α‐tocopherol: 100 µM) or C (ascorbic acid: significantly...
The present study was conducted to investigate the effects of attachment cumulus cells oocytes and coculture with during maturation culture on nuclear status DNA fragmentation porcine denuded (DOs). In first experiment, were removed from cumulus-oocyte complexes (COCs) at 0, 8, 16, 24 or 32 h after onset DOs then cultured in their original droplets until 42 reached. second all COCs before cocultured cells. transferred into fresh medium After culture, examined using terminal...
In this study the efficacy of combination glycerol (GLY) and ethylene glycol (EG) as cryoprotectants in a vitrification method developed for direct embryo transfer was evaluated by vitro development fertilized (IVF) somatic cell nuclear (SCNT) embryos after vitrification. The IVF SCNT blastocysts were vitrified either 40% GLY, 30% GLY + 10% EG, or 20% EG using French straws. After warming, straws held vertically 1 min without shaking then placed horizontally 5 to dilute cryoprotectants....